Biophysical properties of NaV1.7-mediated currents in HEK293 cells, transfected with different NEDD4-2 plasmids, with (+) or without (–) metformin treatment.
| NaV1.7 alone | NaV1.7 + Low [NEDD4-2] | NaV1.7 + NEDD4-2CS | ||||
|---|---|---|---|---|---|---|
| Metformin | – | + | – | + | – | + |
| SSA | ||||||
| V1/2 (mV) | −8.1 ± 0.7 | −6.3 ± 1.1 | −6.7 ± 1.1 | −5.4 ± 1.4 | −9.3 ± 0.6 | −9.1 ± 0.9 |
| Slope | 6.5 ± 0.2 | 7.1 ± 0.3 | 6.0 ± 0.3 | **7.2 ± 0.3 | 7.1 ± 0.3 | 7.1 ± 0.2 |
| n | 30 | 17 | 16 | 11 | 12 | 11 |
| SSI | ||||||
| V1/2 (mV) | −61.3 ± 1.1 | −61.5 ± 1.3 | −58.0 ± 1.5 | −58.9 ± 1.8 | −65.8 ± 1.2 | −65.2 ± 1.3 |
| Slope | 7.45 ± 0.4 | *9.01 ± 0.6 | 8.82 ± 0.7 | 7.75 ± 0.6 | 7.72 ± 0.5 | 7.1 ± 0.3 |
| n | 30 | 16 | 15 | 14 | 12 | 12 |
The three groups correspond to HEK293 cells transfected only with NaV1.7, with NaV1.7 and NEDD4-2 in low concentration (NaV1.7 cDNA/NEDD4-2 cDNA ratio 1:0.3), or with NaV1.7 and an inactivated NEDD4-2, mutated on the catalytic site. The V1/2 (mV) is the voltage at which half of the available channels are activated or inactivated based on their steady-state protocol. Data are expressed as the mean ± SEM. Statistical analysis was performed using an unpaired t test with Welch’s correction (Extended Data Table 1-2). Detailed references for the resources used are presented in Extended Data Table 1-1.
*p < 0.05, **p < 0.01, ***p < 0.001. The bold values correspond to the values, which shows a statistically significant difference.