Primers used for genotyping
| Strain | Gene loci | Primer sequence (5′ to 3′) | Tm |
|---|---|---|---|
| B6;129S-Oxttm1Wsy /J; | Oxt | gtgctggacctggatatgcgcaag | 68 |
| agcgtcctttgccgcccgggccgcaggggagacactgtggctgtgg | |||
| ctgctaaagcgcatgctccagactgc | |||
| Trh KO | Trh | tctcgtcgtgacccatggcgatg | 64 |
| tctcgtcgtgacccatggcgatg | |||
| ttactcctccagaggttccctgac | |||
| B6.129P2-Avpr1a tm1Dgen /J | Avpr 1a | cgcaacgaggagctggcgaagctgg | 64 |
| gcggtaggtgatgtcccagcacagc | |||
| gggccagctcattcctcccactcat | |||
| Oxtr KO | Oxtr | gttgggaacagcggtgatta | 64 |
| ccttggaagcaggaggtgaag | |||
| gctgcgcagtggtggtgacttc | |||
| B6;129×1-Avpr1b tm1Wsy /J | Avpr 1b | accccttcccagcctctgagcccagaaagcgaagg | 64 |
| gaaacggctactctctccgattccaaaagaaag | |||
| acctgtagatatttgacagcccgg | |||
| B6;129Sv-V1atm1GztV1btm1Gzt | Avpr 1a | acaagtgttttgtaactagtgactcta | 60 |
| aggggcttctggtcacgccttgt | |||
| acatagcgttggctacccgtgat | |||
| Avpr 1b | gaaacggctactctctcgattccaaaagaag | 60 | |
| gcgaattcgatatcaagcttatcga | |||
| acctgtagatatttgacagcccgg |