Antibodies and concentrations used
| Target | Supplier | Species raised in | Dilution | Secondary detection | Catalog number | Reference |
|---|---|---|---|---|---|---|
| Kv3.1b | Neuromabs/Antibodies Inc | Mouse | 1:100 | Biotinylated α- mouse IGg1 (Invitrogen A10519), Streptavidin Alexa 555 (Invitrogen) | 75-041 | Soares et al. (2017) |
| Kv3.3 | Neuromabs/Antibodies Inc | Mouse | 1:100 | Biotinylated α- mouse IGg1 (Invitrogen A10519), Streptavidin Alexa 555 (Invitrogen) | 75-354 | Soares et al. (2017) |
| GlyT2 (glycine transporter) | Synaptic Systems | Rabbit | 1:2000 | Donkey α-Rabbit Alexa 488 (Life Technologies, A21206) | 272003 | Jursky and Nelson (1995) |
| VGAT (vesicular GABA transporter) | Synaptic Systems | Rabbit | 1:2000 | Donkey α-Rabbit Alexa 488 (Life Technologies, A21206) | 131003 | Tozuka et al. (2005) |
| VGluT2 (vesicular glutamate transporter) | Synaptic Systems | Rabbit | 1:2000 | Donkey α-Rabbit Alexa 488 (Life Technologies, A21206) | 135403 | Zhu et al. (2018) |
| ChAT (choline acteyltransferase) | Abcam | Goat | 1:1000 | Donkey α-Goat Alexa 488 (Life Technologies, A21206) | Ab18736 | Merolli et al. (2019) |