Summary of additional mouse lines that were screened for Cre-mediated recombination in the retina
| Mouse line | Retinal Cre-mediated recombination | Existing literature |
|---|---|---|
| Crh-ires-Cre (knock-in, JAX #012704) | tdTom expression strong in a small subset of ACs in INL, displaced ACs, and RGCs | Similar recombination patterns reported previously in the mouse retina (Zhu et al., 2014; Park et al., 2018) |
| Crh-cre (transgenic, MMRRC 030850-UCD) | tdTom expression strong in a small subset of ACs in INL and a few displaced ACs | No previous reports in the mouse retina; reported in the brain (Niederkofler et al., 2016) |
| VIP-cre (JAX #010908) | tdTom expression strong in a small subset of ACs | Similar recombination patterns reported previously in the mouse retina (Zhu et al., 2014; Akrouh and Kerschensteiner, 2015; Park et al., 2015; Perez de Sevilla Müller et al., 2019) |
| NEX-cre (a gift from K. Nave) | tdTom expression strong in a subset of ACs | Similar recombination patterns reported previously in the mouse retina (Goebbels et al., 2006; Kay et al., 2011) |
| Pomc-cre (JAX #010714) | tdTom expression strong in patches of Mueller glia and some unidentified inner retinal neurons, including HCs | Previous report had a brief statement of no expression in retina; different methodology used (injection of AAV encoding Cre-dependent reporter; Martersteck et al., 2017) |
| Sst-cre/ERT2 (JAX #010708) | Very weak tdTom expression in Mueller glia | No previous reports in the mouse retina; low efficiency recombination reported in the cerebral cortex (Taniguchi et al., 2011) |
| Mnx1-cre (JAX #006600) | tdTom expression dense and strong in Mueller glia and in some unidentified inner retinal neurons | Similar recombination patterns reported previously in the mouse retina (Ivanova et al., 2010) |
| Oxy-cre (JAX #024234) | Very weak tdTom expression in Mueller glia and a few other inner retinal neurons | Brief mention in the mouse retina, expression in ACs, BCs, and HCs; different methodology (injection of AAV encoding Cre-dependent reporter; Martersteck et al., 2017) |
All mice were crossed to a Cre recombinase-dependent reporter mouse line that expresses tdTomato (Ai9, JAX #007909), and adult mouse retinal sections were prepared/imaged as described in Materials and Methods.