Table 1

Morphological properties of sampled pyramidal neurons

	Pyramidal neurons
	Layer 2/3	Layer 4	Layer 5a	Layer 5b	Layer 6 CTL	Layer 6 CCL
Soma area (μm²)	89.7 ± 13.8	89.6 ± 14.2	121.0 ± 18.2^*†	115.6 ± 12.8	72.8 ± 5.68^{‡‡‡§§}	87.3 ± 12.7^‡
Horizontal span of apical dendrite (μm)^a	247.3 ± 13.8	162.9 ± 85.6	233.0 ± 16.1	359.2 ± 181.8	144.9 ± 38.6^§	195.2 ± 82.0
Height of apical dendrites (μm)^a	321.5 ± 70.4	414.3 ± 27.3	512.4 ± 39.3	746.5 ± 111.4^‡‡	588.4 ± 37.8^†	411.5 ± 83.9^§§§§¶¶
Horizontal span of basal dendrite (μm)	306.9 ± 54.0	324.8 ± 65.8	390.7 ± 53.0	375.0 ± 90.5	273.2 ± 77.2	332.6 ± 50.1
Horizontal span of axon collaterals (mm)^a	0.995 ± 0.188	1.098 ± 0.313	1.447 ± 0.409	1.245 ± 0.171	0.387 ± 0.105**^{††‡‡‡‡§§§}	1.335 ± 0.164^¶¶¶¶
Length of axon collaterals (mm)^b	70.9 ± 33.8	64.6 ± 19.9	64.2 ± 15.0	16.8 ± 7.3	22.8 ± 15.5	62.5 ± 40.0
Varicosity size
Short diameter (μm)	0.48 ± 0.14	0.46 ± 0.16	0.50 ± 0.18	0.48 ± 0.20	0.49 ± 0.15	0.45 ± 0.15
Long diameter (μm)	0.64 ± 0.20	0.64 ± 0.25	0.68 ± 0.24	0.67 ± 0.27	0.62 ± 0.17	0.63 ± 0.22
Area (μm²)	0.24 ± 0.13	0.24 ± 0.17	0.27 ± 0.20	0.26 ± 0.21	0.23 ± 0.13	0.24 ± 0.18
Perimeter (μm)	1.77 ± 0.53	1.74 ± 0.63	1.87 ± 0.63	1.80 ± 0.71	1.75 ± 0.48	1.72 ± 0.55
Number of varicosities (A)	5778 ± 968	5863 ± 1646	5549 ± 1289	2418 ± 1145*^†	2227 ± 1284**^††	5142 ± 1957
Varicosity density (/mm)	89.3 ± 24.0	94.5 ± 25.4	104.1 ± 47.3	148.8 ± 40.2	118.3 ± 28.9	93.2 ± 37.1
Number of apposed varicosities to
PV dendrites (B)	680 ± 162	830 ± 219	635 ± 145	365 ± 121	619 ± 354	898 ± 258
PV cell bodies (C)	42 ± 16	50 ± 24	39 ± 21	19 ± 13	23 ± 18	51 ± 35
B/A (%)	11.7 ± 1.6	14.4 ± 3.4	11.9 ± 1.0	15.9 ± 3.4	27.7 ± 3.1****^{††††‡‡‡‡§§§§}	18.8 ± 1.5**^‡¶¶¶
(B+C)/A (%)	12.4 ± 1.6	15.3 ± 3.4	12.6 ± 1.3	16.6 ± 3.3	28.8 ± 3.2****^{††††‡‡‡‡§§§§}	19.8 ± 1.5**^‡¶¶¶
Apposed varicosities density (/mm)	10.2 ± 1.6	13.9 ± 5.5	10.4 ± 3.8	22.7 ± 3.7	33.4 ± 18.0**^†‡	17.5 ± 6.9
Number of varicosities in compound appositions (D)	224 ± 89	284 ± 54	174 ± 114	120 ± 43	315 ± 205	462 ± 155^*‡§§
D/A (%)	3.8 ± 1.1	5.0 ± 1.0	3.2 ± 1.2	5.1 ± 0.6	14.8 ± 0.2****^{†††‡‡‡‡§§§}	9.7 ± 1.6^*‡
D/B (%)	32.5 ± 7.2	35.0 ± 4.6	26.5 ± 8.7	33.0 ± 4.7	52.6 ± 2.8*^†‡‡§	51.7 ± 7.3*^†‡‡
Compound varicosity density (/mm)	6.9 ± 2.1	9.2 ± 2.0	5.9 ± 2.9	9.2 ± 3.6	30.2 ± 1.2****^{††††§§§§}	16.4 ± 4.7**^{†‡‡¶¶¶¶}

*,^†,^‡,^§,^¶ Significant differences (*,^†,^‡,^§,^¶ p < 0.05; **,^††,^‡‡,^§§,^¶¶ p < 0.01; ***,^†††,^‡‡‡,^§§§,^¶¶¶ p < 0.001; ****,^††††,^‡‡‡‡,^§§§§,^¶¶¶¶ p < 0.0001 by one-way-ANOVA and Tukey's multiple comparison test) from the value of layer 2/3, layer 4, layer 5a, layer 5b, or layer 6 CT-like (CTL) neurons, respectively.
^aThe horizontal span and height of dendrites or axon collaterals were measured in the frontal plane to which all the dendrites were projected.
^bLength of axon collaterals was estimated by multiplying the length of axon collaterals projected onto the frontal plane by 4/π.