Reagents and materials
Names | RRIDs or catalog number |
---|---|
BV-2 cell | YB-ATCC-4255, ATCC |
SH-SY5Y cell | RRID:CVCL_0019 |
HEK293T cell | RRID:CVCL_0063 |
DMEM | 11054001, Gibco |
DMEM/F12 | 11330107, Gibco |
FBS | 16140, Invitrogen |
Penicillin/streptomycin | 15140148, Invitrogen |
LPS | L-4391, Sigma-Aldrich |
Lipofectamine 3000 | L3000150, Invitrogen |
TRIzol | 15596026, Invitrogen |
Reverse transcription kit | 6210A, TaKaRa |
SYBR Green Mix | 2015099, Roche |
RIPA lysis buffer | P0013C, Beyotime |
BCA kit | P0012, Beyotime |
Protein loading buffer | P0015A, Beyotime |
β-Actin antibody | RRID:AB_306371 |
GLRX antibody | RRID:AB_880242 |
Film development kit | P0019, Beyotime |
TNF-α | DY410, R&D |
IL-1β | SMLB00C, R&D |
IL-6 | SM6000B, R&D |
CCK-8 kit | CK04, Dojindo |
Annexin V-FITC cell apoptosis kit | C1062L, Beyotime |
PBS | C0221A, Beyotime |
A + G beads | P2108, Beyotime |
Ago2 antibody | RRID:AB_867543 |
IgG antibody | RRID:AB_2687931 |
pGL3-Promoter | E1761, Promega |
pRL-TK | E2241, Promega |
Dual-Luciferase Reporter Assay System | E1910, Promega |
MPTP | M0896, Sigma-Aldrich |
4% paraformaldehyde | P0099, Beyotime |
Proteinase K | ST535, Beyotime |
Tyrosine hydroxylase antibody | RRID:AB_2801410 |
Iba1 antibody | RRID:AB_2636859 |
DAPI | C1002, Beyotime |
MiRNA mimic | B02003, GenePharma |
MiRNA inhibitor | B03001, GenePharma |
Gene overexpression plasmid | C05001, GenePharma |
MiRNA antagomir | B05001, GenePharma |