Table 3

Reagents and materials

NamesRRIDs or catalog number
BV-2 cellYB-ATCC-4255, ATCC
SH-SY5Y cellRRID:CVCL_0019
HEK293T cellRRID:CVCL_0063
DMEM11054001, Gibco
DMEM/F1211330107, Gibco
FBS16140, Invitrogen
Penicillin/streptomycin15140148, Invitrogen
LPSL-4391, Sigma-Aldrich
Lipofectamine 3000L3000150, Invitrogen
TRIzol15596026, Invitrogen
Reverse transcription kit6210A, TaKaRa
SYBR Green Mix2015099, Roche
RIPA lysis bufferP0013C, Beyotime
BCA kitP0012, Beyotime
Protein loading bufferP0015A, Beyotime
β-Actin antibodyRRID:AB_306371
GLRX antibodyRRID:AB_880242
Film development kitP0019, Beyotime
TNF-αDY410, R&D
IL-1βSMLB00C, R&D
IL-6SM6000B, R&D
CCK-8 kitCK04, Dojindo
Annexin V-FITC cell apoptosis kitC1062L, Beyotime
PBSC0221A, Beyotime
A + G beadsP2108, Beyotime
Ago2 antibodyRRID:AB_867543
IgG antibodyRRID:AB_2687931
pGL3-PromoterE1761, Promega
pRL-TKE2241, Promega
Dual-Luciferase Reporter Assay SystemE1910, Promega
MPTPM0896, Sigma-Aldrich
4% paraformaldehydeP0099, Beyotime
Proteinase KST535, Beyotime
Tyrosine hydroxylase antibodyRRID:AB_2801410
Iba1 antibodyRRID:AB_2636859
DAPIC1002, Beyotime
MiRNA mimicB02003, GenePharma
MiRNA inhibitorB03001, GenePharma
Gene overexpression plasmidC05001, GenePharma
MiRNA antagomirB05001, GenePharma