Table 3

Evaluation of the model performance on images from different preparations

DataPrecisionRecallF1-score (alg.)F1-score (human)
Acute slices77.87 ± 1.15%, n = 1182.34 ± 1.87%, n = 1179.63 ± 1.11%, n = 1183.59 ± 1.71%, n = 11
Cryo-fixed n.c.85.10 ± 1.30%, n = 778.13 ± 4.69%, n = 780.82 ± 2.38%, n = 791.33 ± 0.93%, n = 7
Chemically-fixed n.c.87.53 ± 1.96%, n = 987.24 ± 1.53%, n = 987.22 ± 1.37%, n = 992.99 ± 0.70%, n = 9
  • Performance of the model (precision, recall, and F1-score) on images of hMFBs from chemically-fixed acute hippocampal slices (acute slices) and of small hippocampal synapses from either cryo-fixed or chemically-fixed neuronal cultures (cryo-fixed n.c. and chemically-fixed n.c., respectively; dataset test final) and F1-score obtained comparing the annotations of two humans with each other. Data are presented as mean ± SEM.