Deparaffinization, rehydration, and H&E staining procedure
Steps in H&E staining procedure | ||
---|---|---|
10 min | Xylene I | Deparaffinization |
10 min | Xylene II | |
5 min | Ethanol absolute I | Rehydration |
5 min | Ethanol absolute II | |
5 min | Ethanol 96% I | |
5 min | Ethanol 96% II | |
5 min | Ethanol 70% I | |
5 min | Ethanol 70% II | |
5 min | Ethanol 50% I | |
5 min | Ethanol 50% II | |
5 min | Distilled water | |
10 min | Mayer’s hematoxylin | Nuclear staining |
30 s | Distilled water | |
10 min | Running lukewarm tap water | Bluing |
30 s | Distilled water | |
2 min | Eosin Y 1% | Counterstaining |
30 s | Distilled water | |
30 s | Ethanol 70% | Dehydration |
3 min | Ethanol 96% | |
3 min | Ethanol absolute I | |
3 min | Ethanol absolute II | |
3 min | Roti-Histol I | Clearance before mounting |
3 min | Roti-Histol II | |
Roti-Histokitt | Mounting |