Table 1

Deparaffinization, rehydration, and H&E staining procedure

Steps in H&E staining procedure
10 minXylene IDeparaffinization
10 minXylene II
5 minEthanol absolute IRehydration
5 minEthanol absolute II
5 minEthanol 96% I
5 minEthanol 96% II
5 minEthanol 70% I
5 minEthanol 70% II
5 minEthanol 50% I
5 minEthanol 50% II
5 minDistilled water
10 minMayer’s hematoxylinNuclear staining
30 sDistilled water
10 minRunning lukewarm tap waterBluing
30 sDistilled water 
2 minEosin Y 1%Counterstaining
30 sDistilled water
30 sEthanol 70%Dehydration
3 minEthanol 96%
3 minEthanol absolute I
3 minEthanol absolute II
3 minRoti-Histol IClearance
before mounting
3 minRoti-Histol II
Roti-HistokittMounting