Biological conditions assessed for tissue viability
| Abbreviation | Cryopreservation | NBM complemented with DMSO |
|---|---|---|
| FI | Freshly isolated tissue | No |
| D1w/oD | 1 day cryopreserved | No |
| W1w/oD | 1 week cryopreserved | No |
| D1wD | 1 day cryopreserved | With 8% DMSO |
| W1wD | 1 week cryopreserved | With 8% DMSO |
| M3wD | 3 months cryopreserved | With 8% DMSO |
| Y1wD | 1 year cryopreserved | With 8% DMSO |
NBM, Neurobasal medium; DMSO, Dimethyl sulfoxide