Table 3

Cryopreservation conditions set up

Condition	Preservation medium	Cooling/freezing	W/WO isopentane	Storage	Responding cells (%)
1	NBM	4°C	-	4°C > 6 h	0%
2	NBM	−20°C	WO	−20°C	0%
3	NBM	−80°C	WO	−80°C	0%
4	NBM + 8% DMSO	Snap Liq N₂	WO	Liq N₂	0%
5	NBM + 8% DMSO	−20°C, 10 min	WO	Liq N₂	0%
6	NBM + 8% DMSO	−80°C, 24 h	WO	Liq N₂	0%
7	NBM	−80°C, 5–10 h	W	Liq N₂	<50%
8	NBM + 8% DMSO	−80°C, 5–10 h	W	Liq N₂	>50%

NBM, Neurobasal medium; DMSO, Dimethyl sulfoxide; Liq N2, Liquid nitrogen; W, with; WO, without.