Figure | Data structure | Type of test | Sample size | Statistical data |
---|---|---|---|---|
Figure 1B | One tailed | Unpaired t test | siNEG: 3 experimental replicates siA1#1: 3 experimental replicates siA1#2: 3 experimental replicates siA1#3: 3 experimental replicates siA1#4: 3 experimental replicates | siNEG vs siA1#1 p = 0.1471, t = 1.206, df = 4; siNEG vs siA1#2 p = 0.0277, t = 2.678, df = 4; siNEG vs siA1#3 p = 0.0041, t = 4.871, df = 4; siNEG vs siA1#4 p = 0.0011, t = 6.929, df = 4. |
Figure 1E | One tailed | Unpaired t test | siNEG: 3 experimental replicates siA1: 3 experimental replicates | p = 0.0005, t = 8.435, df = 4, 95% CI = −1.057 to −0.5336, r2 = 0.9468, difference between means ± SEM = −0.7954 ± 0.09429 |
Figure 1G | One tailed | Unpaired t test | siNEG: 3 experimental replicates with over 30 cells analyzed per replicate siA1: 3 experimental replicates with over 30 cells analyzed per replicate | p < 0.0001, t = 10.50, df = 193, 95% CI = −27493 to −18797, r2 = 0.3635, difference between means ± SEM = −23145 ± 2205 |
Figure 4B | One tailed | Unpaired t test | siNEG: 3 experimental replicates with over 90 cells analyzed in total Severe siA1: 3 experimental replicates with 20 cells analyzed in total | p = 0.0002, t = 3.644, df = 116, 95% CI = −2.890 to −0.8547, r2 = 0.1027, difference between the means ± SEM = −1.872 ± 0.5139 |
Figure 4D | One tailed | Unpaired t test | siNEG: 3 experimental replicates with over 90 cells analyzed in total Severe siA1: 3 experimental replicates with 20 cells analyzed in total | p = 0.0002, t = 3.671, df = 116, 95% CI = −62.74 to −18.76, r2 = 0.1041, difference between the means ± SEM = −40.75 ± 11.10 |
Figure 4F | One tailed | Unpaired t test | siNEG: 3 experimental replicates siA1: 3 experimental replicates | p = 0.0221, t = 2.900, df = 4, 95% CI = 0.1822–8.404, r2 = 0.6776 |
Figure 5B | One tailed | Unpaired t test | siNEG: 3 experimental replicates with over 290 cells analyzed in total Severe siA1: 3 experimental replicates with 141 cells analyzed in total | p < 0.0001, t = 7.480, df = 437, 95% CI = −2.641 to −1.542, r2 = 0.1135, difference between the means ± SEM = −2.091 ± 0.2796 |
Figure 5C | One tailed | PC test | siA1: analyzed 300 cells for their hnRNP A1 fluorescence and SG number; each dot represents a single cell | p < 0.0001, r = 0.2825, r2 = 0.07982, 95% CI = 0.1749–0.3835 |
Figure 5D | One tailed | Unpaired t test | siNEG: 3 experimental replicates with over 290 cells analyzed in total siA1: 3 experimental replicates with 141 cells analyzed in total | p < 0.0001, t = 6.494, df = 443, 95% CI = −0.5998 to −0.3211, r2 = 0.08691, difference between the means ± SEM = −0.4605 ± 0.07091 |