Pan-neuronal expression of CG31030 rescues the embryonic lethality of CG31030-deficient flies up to the adult stage
| Genotype of the progeny flies1 | Expected percentage2 | Scored percentage3 |
|---|---|---|
| Heterozygous deficiency without CG31030 expression | 40 | 39.7 |
| Heterozygous deficiency with pan-neuronal CG31030 expression | 40 | 53.8 |
| Homozygous deficiency without CG31030 expression | 0 | 0 |
| Homozygous deficiency with pan-neuronal CG31030 expression | 20 | 6.4 |
↵1 Rescue of embryonic lethality was assayed by crossing elav-Gal4; CG31030MI107/TM6B(Tb) females with w; UAS-CG31030/CyO; Df(3R)Exel6214/TM6B(Tb) males, and scoring the relative number of CG31030-expressing adult flies homozygous for CG31030 deficiency (i.e., non-Tb and non-Cy elav-Gal4/+; UAS-CG31030/+; CG31030MI107/Df(3R)Exel6214) in the progeny (highlighted line).
↵2 Expected percentage of adult progeny flies of each genotype in case of full rescue.
↵3 Actual percentage obtained in the experiments. 24 rescued adults were recovered out of a total of 373 progeny flies.