Timepoint | P30 | P32 | P34 | P38 | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 |
Reads_exon4 | 872 | 975 | 765 | 255 | 648 | 873 | 802 | 919 | 885 | 1156 | 1034 | 858 |
Reads_exon5 | 350 | 333 | 214 | 53 | 177 | 199 | 213 | 247 | 172 | 228 | 329 | 154 |
E5/E4 ratio | 0.40 | 0.34 | 0.28 | 0.21 | 0.27 | 0.23 | 0.27 | 0.27 | 0.19 | 0.20 | 0.32 | 0.18 |
Mean E5/E4 ratio | 0.34 | 0.24 | 0.24 | 0.23 | ||||||||
SD E5/E4 ratio | 0.06 | 0.03 | 0.04 | 0.08 | ||||||||
% reduction E5 | 0.00 | 30.68 | 28.74 | 32.05 |
Shown are absolute RNA-seq read numbers for the closest OTX2 exon 4 and floxed exon 5 at each timepoint. E5/E4 read ratios. mean and SD for each triplicate and the percentage of exon 5 reads reduction after cKO.