Primary antibodies used for immunofluorescent imaging or Western immunoblotting
| Antigen | Dilution | Species | Isotype | Manufacturer information and RRID citation |
|---|---|---|---|---|
| Akt | 1:1000 | Rabbit | IgG | Cell Signaling Technology catalog #9272, RRID:AB_329827 |
| Phospho-Akt (Ser473) | 1:1000 | Rabbit | IgG | Cell Signaling Technology catalog #9271, RRID:AB_329825 |
| AnkyrinG | 1:400 | Mouse | IgG2a | UC Davis/NIH NeuroMab Facility catalog #75-146, RRID:AB_10673030 |
| βIV spectrin | 1:400 | Rabbit | IgG | M. N. Rasband, Baylor College of Medicine, TX, catalog #bIV SD, RRID:AB_2315634 |
| CaMKIIα | 1:400 | Mouse | IgG1 | Thermo Fisher Scientific catalog #MA1-048, RRID:AB_325403 |
| GAPDH | 1:1000 | Mouse | IgG1 | Enzo Life Sciences catalog #ADI-CSA-335-E, RRID:AB_2039148 |
| GFAP | 1:1000 | Mouse | IgG1 | UC Davis/NIH NeuroMab Facility catalog #75-240, RRID:AB_10672299 |
| MAP2 | 1:1000 | Chicken | IgY | EnCor Biotechnology catalog #CPCA-MAP2, RRID:AB_2138173 |
| MG-H1 | 1:1000 | Rat | IgG | T. Fleming and P. Nawroth, Heidelberg University, Germany; 1D2 tissue culture supernatant |