List of primary and secondary antibodies used for immunohistochemistry and western blot
| Antigen | Host | Supplier | Catalog no. | Dilution | |
|---|---|---|---|---|---|
| IHC | WB | ||||
| Primary antibodies | |||||
| α-Tubulin | Mouse | Sigma-Aldrich | T5168 | 1:200.000 | |
| GSK3α/β | Mouse | Thermo Fisher Scientific | 44–610 | 1:250 | 1:1000 |
| GSK3β | Mouse | BD | 610201 | 1:250 | |
| Brn3a | Mouse | Santa Cruz Biotechnology | sc-8429 | 1:200 | |
| Calbindin D-28k | Rabbit | Swant | 300 | 1:100 | |
| Calretinin | Mouse | EMD Millipore | MAB1568 | 1:1000 | |
| Cone arrestin | Rabbit | EMD Millipore | AB15282 | 1:1000 | |
| Rhodopsin | Mouse | Abcam | MAB5316 | 1:2000 | |
| Tbr2 | Rat | Thermo Fisher Scientific | 14–4876 | 1 :300 | |
| Foxp2 | Goat | Santa Cruz Biotechnology | sc-21069 | 1 :1000 | |
| Rbpms | Rabbit | PhosphoSolutions | 1830-RBPMS | 1 :400 | |
| CHAT | Goat | Millipore | AB144P | 1 :100 | |
| Secondary antibodies | |||||
| Alexa Fluor-555 anti-mouse IgG2A | Goat | Thermo Fisher Scientific | A21127 | 1:1000 | |
| Alexa Fluor-555 anti-mouse IgG2B | Goat | Thermo Fisher Scientific | A21147 | 1:1000 | |
| Alexa Fluor-488 anti-rabbit | Donkey | Thermo Fisher Scientific | A21206 | 1:1000 | |
| Alexa Fluor-488 anti-mouse IgG1 | Goat | Thermo Fisher Scientific | A21240 | 1:1000 | |
| Alexa Fluor-488 anti-rabbit | Goat | Thermo Fisher Scientific | A21244 | 1:1000 | |
| HRP anti-mouse IgG | Goat | Sigma-Aldrich | A4416 | 1:5000 | |
| Alexa Fluor-488 anti-goat | Donkey | Thermo Fisher Scientific | A11055 | 1:1000 | |
WB, Western blot; IHC, Immunohistochenistry.