Table 2

Properties of spontaneous burst firing in R-R, R-C, and R-H LNs

R-R/FM7R-C/FM7R-H/FM7
Burst frequency, Hz22˚C: 0.70 ± 0.2422˚C: 0.90 ± 0.2622˚C: 0.70 ± 0.24
27˚C: 0.63 ± 0.2627˚C: 0.57 ± 0.1727˚C: 0.63 ± 0.26
22˚C: 0.57 ± 0.2022˚C: 0.37 ± 0.1622˚C: 0.57 ± 0.20
Action potential threshold, mV22˚C: −37.77 ± 1.4822˚C: −37.48 ± 1.8322˚C: −38.00 ± 2.45
27˚C: −36.74 ± 1.3627˚C: −37.06 ± 1.7727˚C: −33.81 ± 3.10
22˚C: −36.79 ± 1.6622˚C: −35.95 ± 2.0822˚C: −36.17 ± 2.40
Action potential amplitude, mV22˚C: 35.34 ± 2.3522˚C: 32.27 ± 3.8322˚C: 28.18 ± 5.16
27˚C: 28.29 ± 2.3427˚C: 23.16 ± 3.1927˚C: 23.33 ± 3.87
22˚C: 34.27 ± 3.8122˚C: 28.52 ± 2.5722˚C: 31.33 ± 4.16
Action potentials per burst22˚C: 5.27 ± 0.3922˚C: 5.24 ± 0.5622˚C: 4.79 ± 0.35
27˚C: 5.46 ± 0.3827˚C: 5.05 ± 0.5627˚C: 4.34 ± 0.87
22˚C: 6.17 ± 0.5022˚C: 5.31 ± 0.3922˚C: 5.23 ± 0.49
Resting membrane potential, mV22˚C: −57.1 ± 2.222˚C: −54.2 ± 2.022˚C: −54.0 ± 2.6
27˚C: −55.9 ± 1.827˚C: −52.1 ± 1.327˚C: −47.8 ± 3.2*
30˚C: −57.4 ± 2.430˚C: −58.3 ± 4.030˚C: −47.9 ± 1.9*
22˚C: −54.9 ± 3.222˚C: −49.5 ± 2.122˚C: −49.8 ± 1.8
  • Values are the mean ± SE at 22°C (during the 5 min preheating), “27°C” (during the minute of heating from 26°C to 29°C), and again at 22°C (during the 5 min postcooling).

  • * R-H mutants have depolarized RMP at elevated temperatures compared with R-R controls; p < 0.05, two-way ANOVA.