Properties of spontaneous burst firing in R-R, R-C, and R-H LNs
R-R/FM7 | R-C/FM7 | R-H/FM7 | |
---|---|---|---|
Burst frequency, Hz | 22˚C: 0.70 ± 0.24 | 22˚C: 0.90 ± 0.26 | 22˚C: 0.70 ± 0.24 |
27˚C: 0.63 ± 0.26 | 27˚C: 0.57 ± 0.17 | 27˚C: 0.63 ± 0.26 | |
22˚C: 0.57 ± 0.20 | 22˚C: 0.37 ± 0.16 | 22˚C: 0.57 ± 0.20 | |
Action potential threshold, mV | 22˚C: −37.77 ± 1.48 | 22˚C: −37.48 ± 1.83 | 22˚C: −38.00 ± 2.45 |
27˚C: −36.74 ± 1.36 | 27˚C: −37.06 ± 1.77 | 27˚C: −33.81 ± 3.10 | |
22˚C: −36.79 ± 1.66 | 22˚C: −35.95 ± 2.08 | 22˚C: −36.17 ± 2.40 | |
Action potential amplitude, mV | 22˚C: 35.34 ± 2.35 | 22˚C: 32.27 ± 3.83 | 22˚C: 28.18 ± 5.16 |
27˚C: 28.29 ± 2.34 | 27˚C: 23.16 ± 3.19 | 27˚C: 23.33 ± 3.87 | |
22˚C: 34.27 ± 3.81 | 22˚C: 28.52 ± 2.57 | 22˚C: 31.33 ± 4.16 | |
Action potentials per burst | 22˚C: 5.27 ± 0.39 | 22˚C: 5.24 ± 0.56 | 22˚C: 4.79 ± 0.35 |
27˚C: 5.46 ± 0.38 | 27˚C: 5.05 ± 0.56 | 27˚C: 4.34 ± 0.87 | |
22˚C: 6.17 ± 0.50 | 22˚C: 5.31 ± 0.39 | 22˚C: 5.23 ± 0.49 | |
Resting membrane potential, mV | 22˚C: −57.1 ± 2.2 | 22˚C: −54.2 ± 2.0 | 22˚C: −54.0 ± 2.6 |
27˚C: −55.9 ± 1.8 | 27˚C: −52.1 ± 1.3 | 27˚C: −47.8 ± 3.2* | |
30˚C: −57.4 ± 2.4 | 30˚C: −58.3 ± 4.0 | 30˚C: −47.9 ± 1.9* | |
22˚C: −54.9 ± 3.2 | 22˚C: −49.5 ± 2.1 | 22˚C: −49.8 ± 1.8 |
Values are the mean ± SE at 22°C (during the 5 min preheating), “27°C” (during the minute of heating from 26°C to 29°C), and again at 22°C (during the 5 min postcooling).
↵* R-H mutants have depolarized RMP at elevated temperatures compared with R-R controls; p < 0.05, two-way ANOVA.