Table 1

Hypothesis testing statistics table showing the statistical tests used in the study and respective results

FigurePanelMeasureGroups (sample size)TestDetailsStatisticp value
1BEffect of secretory IgM on the % area of IgG deposition at 2 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA Sidak’s post hoc to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 21.31<0.0001
Section −1200 μmt = 3.5280.0078
Section −600 μmt = 3.5130.0082
Section 600 μmt = 2.9570.0445
CEffect of secretory IgM on the % area of IgG deposition at 10 weeks post-SCIKO (4), WT (4) miceTwo-way ANOVA
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 1.9650.1657
HEffects of secretory IgM on levels of serum IgG immunoglobulinKO: BSL (9),
2 weeks (5),
10 weeks (7) mice		One-way ANOVA
Holm–Sidak’s multiple comparisons test		One-way ANOVA between WT and KOF = 6.6940.0002
WT: BSL (10),
2 weeks (6),
10 weeks (6) mice		BSL: (KO vs WT)t = 0.7600.6995
2 weeks post-SCI:
(KO vs WT)		t = 1.9500.1662
10 weeks post-SCI:
(KO vs WT)		t = 0.3300.7431
2BEffect of secretory IgM on the % area of CD3+ cell infiltration at 2 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Sidak’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 15.930.0001
Section −1200 μmt = 2.6890.0088
Section −600 μmt = 2.8620.0054
Section 0t = 2.290.0248
Section 600 μmt = 2.7760.0069
Effect of secretory IgM on the % area of CD3+ cell infiltration at 10 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 0.8270.3659
CEffect of secretory IgM on CD3+ cell counts at 2 weeks post-SCIKO (5), WT (5) miceTwo-way ANOVA
Sidak’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 9.3940.0029
Section −1200 μmt = 2.0000.0486
Section −600 μmt = 2.0950.039
Section 0t = 2.2750.0253
Section 600 μmt = 2.4830.0149
Effect of secretory IgM on CD3+ cell counts at 10 weeks post-SCIKO (5), WT (5) miceTwo-way ANOVA
Sidak’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 7.3350.0081
Section −1800 μmt= 2.3510.021
3BEffect of secretory IgM on the % area of CD11b+ cells at 2 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Sidak’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 20.69<0.0001
Section −1200 μmt = 2.4710.0157
Section 0t = 3.0310.0033
Section 600 μmt = 2.8000.0065
Effect of secretory IgM on the % area of CD11b+ cell at 10 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Sidak’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotype
No significant post hoc difference		F = 5.0690.0272
CEffect of secretory IgM on CD11b+ cell counts at 2 weeks post-SCIKO (5), WT (5) miceTwo-way ANOVA
Sidak’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 21.61<0.0001
Section −1200 μmt = 2.0960.039
Section 600 μmt = 2.5660.012
Effect of secretory IgM on CD11b+ cell counts at 10 weeks post-SCIKO (5), WT (5) miceTwo-way ANOVA
Sidak’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 21.92<0.0001
Section −2400 μmt = 2.5730.0117
Section −1200 μmt = 2.4210.0175
Section −600 μmt = 2.6470.0096
Section 1200 μmt = 2.0740.041
4BEffect of secretory IgM on % lesional tissue at 2 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 2.6810.1056
CEffect of secretory IgM on % white matter preservation at 2 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 0.1990.6566
DEffect of secretory IgM on % gray matter preservation at 2 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Fisher’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 6.5440.0061
Section 0t = 2.8170.0062
EEffect of secretory IgM on % lesional tissue at 10 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Fisher’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 22.53<0.0001
Section −2400 μmt = 2.0410.0452
Section −1200 μmt = 2.2160.0302
Section −600 μmt = 3.3280.0014
4FEffect of secretory IgM on % white matter preservation at 10 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Fisher’s post hoc
to compare between KO and WT mice for each distance point
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 33.38<0.0001
Section −2400 μmt = 2.5790.0121
Section −1200 μmt = 2.5740.0123
Section −60 μmt = 2.9180.0048
Section 1800 μmt = 2.4960.0151
Section 2400 μmt = 2.3310.0228
Section 3000 μmt = 2.0280.0466
GEffect of secretory IgM on the % gray matter preservation at 10 weeks post-SCIKO (4), WT (5) miceTwo-way ANOVA
Factor 1 (genotype), factor 2 (distance from epileft)		Effect of genotypeF = 0.14370.7059
5CEffect of secretory IgM on step sequence regularity at 4, 8, and 10 weeks post-SCIThree-way ANOVA
One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA
Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI)		Effect of genotype × injuryF = 10.230.0019
4 weeks:
KO SCI (10),
KO sham (10)
WT SCI (7),
WT sham (10)
mice		4 weeks one-way
ANOVA		F = 9.6250.0001
KO SCI vs KO shamt = 3.9850.0011
KO SCI vs WT SCIt = 0.4270.9648
WT SCI vs WT shamt = 3.4680.0044
8 weeks:
KO SCI (10),
KO sham (8),
WT SCI (7),
WT sham (9) mice		8 weeks one-way
ANOVA		F = 36.14<0.0001
KO SCI vs KO shamt = 36.14<0.0001
KO SCI vs WT SCIt = 3.6750.0028
WT SCI vs WT shamt = 4.3620.0004
10 weeks:
KO SCI (7),
KO sham (8),
WT SCI (6),
WT sham (8) mice		10 weeks one-way
ANOVA		F = 28.85<0.0001
KO SCI vs KO shamt = 8.244<0.0001
KO SCI vs WT SCIt = 4.4150.0005
WT SCI vs WT shamt = 3.0650.0154
DEffect of secretory IgM on hindlimb swing speed at 4, 8, and 10 weeks post-SCIThree-way ANOVA
One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA
Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI)		Effect of genotype × injuryF = 5.3510.0229
4 weeks:
KO SCI (10),
KO sham (10)
WT SCI (7),
WT sham (10)
mice		4 weeks one-way
ANOVA		F= 2.6060.0682
KO SCI vs KO shamt = 2.5350.0477
KO SCI vs WT SCIt = 1.0150.682
WT SCI vs WT shamt = 0.9790.7057
5DEffect of secretory IgM on hindlimb swing speed at 4, 8, and 10 weeks post-SCI8 weeks:
KO SCI (10),
KO sham (8),
WT SCI (7),
WT sham (9) mice		8 weeks one-way
ANOVA		F = 3.2140.0358
KO SCI vs KO shamt = 2.4230.0624
KO SCI vs WT SCIt = 2.3720.0699
WT SCI vs WT shamt = 0.1260.999
10 weeks:
KO SCI (9),
KO sham (8),
WT SCI (6),
WT sham (8) mice		10 weeks one-way ANOVAF = 5.7330.0127
KO SCI vs KO shamt = 3.1230.0395
KO SCI vs WT SCIt = 2.4740.3076
WT SCI vs WT shamt = 1.040.668
EEffect of secretory IgM on forelimb swing speed at 4, 8, and 10 weeks post-SCIThree-way ANOVA
One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA
Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI)		Effect of genotype × injuryF = 0.9980.3205
4 weeks:
KO SCI (10),
KO sham (10),
WT SCI (7),
WT sham (10) mice		4 weeks one-way
ANOVA		F = 5.9120.0024
KO SCI vs KO shamt = 3.4670.0044
KO SCI vs WT SCIt = 0.1390.9987
WT SCI vs WT shamt = 2.3370.075
8 weeks:
KO SCI (12),
KO sham (8),
WT SCI (7),
WT sham (9) mice		8 weeks one-way
ANOVA		F = 9.0140.0002
KO SCI vs KO shamt = 4.3640.0004
KO SCI vs WT SCIt = 0.1070.9994
WT SCI vs WT shamt = 2.8230.0242
10 weeks:
KO SCI (9),
KO sham (8),
WT SCI (6),
WT sham (8) mice		10 weeks one-way
ANOVA		F = 6.610.0017
KO SCI vs KO shamt = 3.4180.006
KO SCI vs WT SCIt = 0.7770.8278
WT SCI vs WT shamt = 2.5950.0446
FEffect of secretory IgM on hindlimb print area at 4, 8, and 10 weeks post-SCIThree-way ANOVA
One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA
Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI)		Effect of genotype × injuryF = 1.4160.2371
4 weeks:
KO SCI (10),
KO sham (10),
WT SCI (7),
WT sham (10) mice		4 weeks one-way
ANOVA		F = 0.6410.5941
KO SCI vs KO shamt = 0.2390.9934
KO SCI vs WT SCIt = 1.0820.6375
WT SCI vs WT shamt = 1.0950.6291
5FEffect of secretory IgM on hindlimb print area at 4, 8, and 10 weeks post-SCI8 weeks:
KO SCI (12),
KO sham (8),
WT SCI (7),
WT sham (9) mice		8 weeks one-way
ANOVA		F = 1.10.3635
KO SCI vs KO shamt = 0.3400.9817
KO SCI vs WT SCIt = 1.3930.4349
WT SCI vs WT shamt = 0.5460.9306
10 weeks:
KO SCI (9),
KO sham (8),
WT SCI (6),
WT sham (8) mice		10 weeks one-way
ANOVA		F = 8.7870.0003
KO SCI vs KO shamt = 2.310.0838
KO SCI vs WT SCIt = 4.3990.0005
WT SCI vs WT shamt = 0.4670.955
GEffect of secretory IgM on forelimb print area at 4, 8, and 10 weeks post-SCIThree-way ANOVA
One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA
Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI)		Effect of genotype × injuryF = 0.6760.4132
4 weeks:
KO SCI (10),
KO sham (10),
WT SCI (7),
WT sham (10) mice		4 weeks one-way
ANOVA		F = 0.9270.4387
KO SCI vs KO shamt = 0.8080.8098
KO SCI vs WT SCIt = 0.9680.7129
WT SCI vs WT shamt = 0.5260.937
8 weeks:
KO SCI (12),
KO sham (8),
WT SCI (7),
WT sham (9) mice		8 weeks one-way
ANOVA		F = 3.5580.025
KO SCI vs KO shamt = 0.6610.8846
KO SCI vs WT SCIt = 1.1110.6188
WT SCI vs WT shamt = 3.1210.0114
10 weeks:
KO SCI (9),
KO sham (8),
WT SCI (6),
WT sham (8) mice		10 weeks one-way
ANOVA		F = 7.9780.0006
KO SCI vs KO shamt = 2.6360.0406
KO SCI vs WT SCIt = 1.2310.5416
WT SCI vs WT shamt = 3.0680.0145
HEffect of secretory IgM on average body speed at 4, 8, and 10 weeks post-SCIThree-way ANOVA
One-way ANOVA for groups within each time point Sidak’s multiple comparisons post hoc test on one-way ANOVA
Factor 1 (genotype), factor 2 (injury status), factor 3 (time post-SCI)		Effect of genotype × injuryF = 0.5580.457
4 weeks:
KO SCI (10),
KO sham (10),
WT SCI (7),
WT sham (10) mice		4 weeks one-way ANOVAF = 11.75<0.0001
KO SCI vs KO shamt = 4.2750.0005
KO SCI vs WT SCIt = 0.7570.8374
WT SCI vs WT shamt = 4.0990.0008
5HEffect of secretory IgM on average body speed at 4, 8, and 10 weeks post-SCI8 weeks:
KO SCI (12),
KO sham (8),
WT SCI (7),
WT sham (9) mice		8 weeks one-way
ANOVA		F = 13.56<0.0001
KO SCI vs KO shamt = 5.539<0.0001
KO SCI vs WT SCIt = 1.3530.46
WT SCI vs WT shamt = 2.870.0215
10 weeks:
KO SCI (9),
KO sham (8),
WT SCI (6),
WT sham (8) mice		10 weeks one-way
ANOVA		F = 10.080.0001
KO SCI vs KO shamt = 3.9720.0014
KO SCI vs WT SCIt = 0.6780.8776
WT SCI vs WT shamt = 3.5070.0048

  • p values <0.05 are shown in bold for emphasis.