Iba1-EGFP Spinal cord and dorsal root ganglion flow cytometry results
| Wild type | EGFP+/+ | Figure reference | |
|---|---|---|---|
| PN7 spinal cord | (n = 1) | (n = 2) | |
| EGFP+ cells (mean counts ± SD, % live singlets) | 112, 0.2% | 35,385 ± 3896, 59.8 ± 8.8% | Figure 4A |
| Subgate: proportion of EGFP+ cells that are CD11b+/CD45+ (counts ± SD, % EGFP+ ± % SD) | N/A | 35,105 ± 4094, 99.15 ± 0.6% | |
| CD11b+/CD45+ cells (counts/live singlets, % live singlets) | 40163, 78.4% | 43,770 ± 2592, 73.9 ± 7.1% | Figure 4B |
| Subgate: proportion of CD11b+/CD45+ cells that are EGFP+ (counts ± SD, % CD11b+/CD45+ ± % SD) | 111, 0.3% | 35,755 ± 4207, 80 ± 4.6% | |
| PN7 dorsal root ganglion | (n = 1) | (n = 3) | |
| EGFP+ cells (mean counts ± SD, % live singlets) | 170, 0.8% | 2350 ± 240, 6.18 ± 2.0% | Figure 4C |
| Subgate: proportion of EGFP+ cells that are CD11b+/ CD45+ (counts ± SD, % EGFP+ ± % SD) | N/A | 2292 ± 224, 97.6 ± 0.7% | |
| CD11b+/CD45+ cells (counts/live singlets, % live singlets) | 5308, 24.8% | 7162 ± 993, 18.3 ± 2.7% | Figure 4D |
| Subgate: proportion of CD11b+/CD45+ cells that are EGFP+ (counts ± SD, % CD11b+/CD45+ ± % SD) | 167, 3.2% | 2289 ± 226, 32.4 ± 6.1% |