Table 3

Iba1-EGFP Flow cytometry results

Wild typeEGFP+/+Figure
reference
PN7(n = 4)(n = 6)
EGFP+ cells (mean counts ± SD, % live singlets)38 ± 1, 0.1 ± 0.0%6039 ± 440, 9.8 ± 0.7%Figure 2A
Subgate: proportion of EGFP+ cells that are
CD11b+/CD45int (counts ± SD, % EGFP+ ± % SD)
N/A5754 ± 402, 95.3 ± 6.7%
CD11b+/CD45int cells (counts/live singlets, % live singlets)5111 ± 174, 8.3 ± 0.3%6054 ± 324, 9.8 ± 0.5%Figure 2B
Subgate: proportion of CD11b+/CD45int cells
that are EGFP+ (counts ± SD, % CD11b+/
CD45int ± % SD)
33 ± 2, 0.7 ± 0.0%5745 ± 402, 94.9 ± 6.6%
Adult(n = 2)(n = 3)
EGFP+ cells (mean counts ± SD, % live singlets)594 ± 291, 0.55 ± 0.35%25,270 ± 11,127, 37.6 ± 23.6%N/A
Subgate: proportion of EGFP+ cells that are
CD11b+/CD45int (counts ± SD, % EGFP+ ± % SD)
N/A22,766 ± 10,203, 89.8 ± 1.46%
CD11b+/CD45int cells (counts/live singlets, %
live singlets)
21,995 ± 5699, 19.3 ± 1.73%23,892 ± 10,768, 36 ± 22%Figure 2D
Subgate: proportion of CD11b+/CD45int cells that
are EGFP+ (counts ± SD, % CD11b+/CD45int ± % SD)
210 ± 28, 0.97 ± 0.1%22,624 ± 10,085, 94.8 ± 1.4%