Table 5

Statistical analysis of the behavioral experiments in P21 and adult males

Behavioral test	Sex	Age	Normal data?	Equal variance?	Test	Results
Open field test: total distance day 2	M	P21	Yes	Yes	One-way ANOVA	F_(2,72) = 5.017; p = 0.0091 Post hoc Tukey’s test: WT^SGH vs WT^MGH; p = 0.0063 WT^SGH vs KO; p = 0.2796 WT^MGH vs KO; p = 0.1468
Open field test: total distance day 2	M	P60	Yes	Yes	One-way ANOVA	F_(2,68) = 1.13; p = 0.329
Open field test: intervals day 2: 0–5 min	M	P21	No	Yes	Kruskal–Wallis	H₍₂₎ = 9.354; p = 0.0093 Post hoc Dunn’s test: WT^SGH vs WT^MGH; p = 0.0079 WT^SGH vs KO; p = 0.1711 WT^MGH vs KO; p = 0.4797
Open field test: intervals day 2: 5–10 min	M	P21	Yes	Yes	One-way ANOVA	F_(2,72) = 0.719; p = 0.491
Open field test: intervals day 2: 10–15 min	M	P21	Yes	Yes	One-way ANOVA	F_(2,72) = 0.976; p = 0.382
Open field test: intervals day 2: 15–20 min	M	P21	Yes	Yes	One-way ANOVA	F_(2,72) = 2.184; p = 0.12
Open field test: intervals day 2: 0–5 min	M	P60	Yes	Yes	One-way ANOVA	F_(2,68) = 1.312; p = 0.276
Open field test: intervals day 2: 5–10 min	M	P60	Yes	Yes	One-way ANOVA	F_(2,68) = 1.292; p = 0.2813
Open field test: intervals day 2: 10–15 min	M	P60	Yes	Yes	One-way ANOVA	F_(2,68) = 0.13; p = 0.879
Open field test: intervals day 2: 15–20 min	M	P60	No	Yes	Kruskal Wallis	H₍₂₎ = 1.56; p = 0.4584
Open field test: time in left day 2	M	P21	No	Yes	Kruskal Wallis	H₍₂₎ = 2.7579; p = 0.2518
Open field test: time in left day 2	M	P60	No	Yes	Kruskal Wallis	H₍₂₎ = 3.2761; p = 0.1671
24 h activity: total	M	>P60	Yes	Yes	One-way ANOVA	F_(2,34) = 0.4831; p = 0.6210
24 h activity: light period	M	>P60	Yes	Yes	One-way ANOVA	F_(2,34) = 3.031; p = 0.0615
24 h activity: dark period	M	>P60	Yes	Yes	One-way ANOVA	F_(2,34) = 0.3135; p = 0.7330
Elevated plus maze	M	P21	Yes	Yes	One-way ANOVA	F_(2,72) = 1.994; p = 0.144
Elevated plus maze	M	P60	Yes	Yes	One-way ANOVA	F_(2,68) = 6.879; p = 0.0019 Post hoc Tukey’s test: WT^SGH vs WT^MGH; p = 0.9893 WT^SGH vs KO; p = 0.0042 WT^MGH vs KO; p = 0.0138
Social interaction	M	P21	Yes	Yes	One-way ANOVA	F_(2,72) = 2.911; p = 0.039 Post hoc Dunnett’s test (all vs WT SGH): WT^SGH vs WT^MGH; p = 0.0382 WT^SGH vs KO; p = 0.0771

The table includes the behavioral test analyzed, sex and age of the animals, data and variance distribution, statistical test used, and results obtained. M, Male.