Table 6

Statistical analysis of the behavioral experiments in P21 and adult females

Behavioral test	Sex	Age	Normal data?	Equal variance?	Test	Results
Open field test: total distance day 2	F	P21	Yes	Yes	One-way ANOVA	F_(2,69) = 9.539; p = 0.0002 Post hoc Tukey’s test: WT^SGH vs WT^MGH; p = 0.0382 WT^SGH vs HET; p = 0.0001 WT^MGH vs HET; p = 0.0837
Open field test: total distance day 2	F	P60	Yes	Yes	One-way ANOVA	F_(2,69) = 3.990; p = 0.0229 Post hoc Tukey’s test: WT^SGH vs WT^MGH; p = 0.1094 WT^SGH vs HET; p = 0.0214 WT^MGH vs HET; p = 0.6459
Open field test: intervals day 2: 0–5 min	F	P21	No	Yes	Kruskal–Wallis	H₍₂₎ = 21.86; p < 0.0001 Post hoc Dunn’s test: WT^SGH vs WT^MGH; p = 0.0055 WT^SGH vs HET; p < 0.0001 WT^MGH vs HET; p = 0.2018
Open field test: intervals day 2: 5–10 min	F	P21	Yes	Yes	One-way ANOVA	F_(2,69) = 3.290; p = 0.0432 Post hoc Tukey’s test: WT^SGH vs WT^MGH; p = 0.5888 WT^SGH vs HET; p = 0.0359 WT^MGH vs HET; p = 0.2036
Open field test: intervals day 2: 10–15 min	F	P21	Yes	Yes	One-way ANOVA	F_(2,69) = 2.102; p = 0.13
Open field test: intervals day 2: 15–20 min	F	P21	Yes	Yes	One-way ANOVA	F_(2,69) = 1.038; p = 0.36
Open field test: intervals day 2: 0–5 min	F	P60	Yes	Yes	One-way ANOVA	F_(2,69) = 17.95; p < 0.0001 Post hoc Tukey’s test: WT^SGH vs WT^MGH; p < 0.0001 WT^SGH vs HET; p < 0.0001 WT^MGH vs HET; p = 0.9276
Open field test: intervals day 2: 5–10 min	F	P60	Yes	Yes	One-way ANOVA	F_(2,69) = 0.228; p = 0.797
Open field test: intervals day 2: 10–15 min	F	P60	Yes	Yes	One-way ANOVA	F_(2,69) = 1.068; p = 0.349
Open field test: intervals day 2: 15–20 min	F	P60	No	Yes	Kruska–Wallis	H₍₂₎ = 3.2334; p = 0.1986
Open field test: time in left day 2	F	P21	No	Yes	Kruska–Wallis	H₍₂₎ = 4.6819; p = 0.0962
Open field test: time in left day 2	F	P60	No	Yes	Kruska–Wallis	H₍₂₎ = 4.0863; p = 0.1296
24 h activity: total	F	>P60	Yes	Yes	One-way ANOVA	F_(2,36) = 1.077; p = 0.3512
24 h activity: light period	F	>P60	Yes	Yes	One-way ANOVA	F_(2,36) = 2.290; p = 0.1159
24 h activity: dark period	F	>P60	Yes	Yes	One-way ANOVA	F_(2,36) = 1.103; p = 0.3429
Elevated plus maze	F	P21	No	Yes	Kruskal–Wallis	H₍₂₎ = 20.943; p < 0.001 Post hoc Dunn’s test: WT^SGH vs WT^MGH; p = 0.8101 WT^SGH vs HET; p = 0.0042 WT^MGH vs HET; p < 0.0001
Elevated plus maze	F	P60	Yes	Yes	One-way ANOVA	F_(2,69) = 5.085; p = 0.0041 Post hoc Tukey’s test: WT^SGH vs WT^MGH; p = 0.5689 WT^SGH vs HET; p = 0.0043 WT^MGH vs HET; p = 0.0401
Social interaction	F	P21	Yes	Yes	One-way ANOVA	F_(2,69) = 1.297; p = 0.2425
Social interaction	F	P60	Yes	Yes	One-way ANOVA	F_(2,69) = 3.536; p = 0.0398 Post hoc Dunnett’s test (all vs WT SGH): WT^SGH vs WT^MGH; p = 0.0432 WT^MGH vs HET; p = 0.9654

The table includes the behavioral test analyzed, sex and age of the animals, data and variance distribution, statistical test used, and results obtained. F, female.