Table 2

Statistics

DataData structure
(normality test)
Type of testPower
Figure 2D, lysate measurement of Nluc-GPC4 with and without PI-PLC treatmentYesUnpaired t testCohen’s d = 7.29, p < 0.0001 
Figure 2D, media measurement of Nluc-GPC4 with and without PI-PLC treatmentYesUnpaired t testCohen’s d = 3.00, p < 0.0001
Figure 2E, overnight release of GPC4 compared with Prion, normalized to lysateYesUnpaired t testCohen’s d = 3.59, p < 0.0001
Figure 3A, live surface Biotinylation of GPC4 release over timeYesANOVA; Tukey’s multiple
comparisons
Cohen’s d = 1.012, p < 0.0001
Figure 3B, ultracentrifugation of Nluc-GPC4 in ACMsYesUnpaired t testCohen’s d = 7.829, p < 0.0001
Figure 3C, pulldown of Nluc-GPC4 by α ToxinYesANOVA; Tukey’s multiple
comparisons
Cohen’s d = 7.659, p < 0.0001
Figure 4A, reduction of Nluc-GPC4 by protease inhibitor GM6001YesUnpaired t testCohen’s d = 1.118, p = 0.0003
Figure 4B, reduction of Nluc-GPC4 release by Adam9 shRNA1 KDYesANOVA; Tukey’s multiple
comparisons
Cohen’s d = 1.832, p < 0.0001
Figure 4B, reduction of Nluc-GPC4 release by Adam9 shRNA2 KDYesANOVA; Tukey’s multiple
comparisons
Cohen’s d = 2.63, p < 0.0001
Figure 4C, fold gene expression by qPCR for shRNA KD experiments Adam9 shRNA1YesOne-sample t testCohen’s d = 1.393, p = 0.0190
Figure 4C, fold gene expression by qPCR for shRNA KD experiments Adam9 shRNA2YesOne-sample t testCohen’s d = 0.799, p = 0.1076
Figure 4C, fold gene expression by qPCR for shRNA KD experiments Adam10 shRNA1YesOne-sample t testCohen’s d = 10.33, p < 0.0001
Figure 4C, fold gene expression by qPCR for shRNA KD experiments Adam10 shRNA2YesOne-sample t testCohen’s d = 1.089, p = 0.0444
Figure 4D, induction of Nluc-GPC4 release by Adam9 OverexpressionYesANOVA; Tukey’s multiple
comparisons
Cohen’s d = 3.482, p < 0.0001
Figure 5A, heparinase treatment of Nluc-GPC4 vs ControlYesOne-sample t testCohen’s d = 2.93, p = 0.0365
Figure 5B, pulldown of Nluc-GPC4 by α Toxin in PI-PLC control vs basal release conditionsYesUnpaired t testCohen’s d = 0.390, p = 0.9467
Extended Data Figure 1-1, cytotoxicity assay of PI-PLC treatmentYesANOVA; Tukey’s multiple
comparisons
Cohen’s d = 0.907, p = 0.964
Extended Data Figure 1-1, cytotoxicity assay of Edelfosine treatmentYesANOVA; Tukey’s multiple
comparisons
Cohen’s d = 2.870, p < 0.0001
Extended Data Figure 2-1, lysate measurement of Nluc-GPC4-TM with and without PI-PLC treatmentYesUnpaired t testCohen’s d = 0.49, p = 0.19
Extended Data Figure 2-1, media measurement of Nluc-GPC4 = TM with and without PI-PLC treatmentYesUnpaired t testCohen’s d = 0.50, p = 0.27