Data | Data structure (normality test) | Type of test | Power |
---|---|---|---|

Figure 2D, lysate measurement of Nluc-GPC4 with and without PI-PLC treatment | Yes | Unpaired t test | Cohen’s d = 7.29, p < 0.0001 |

Figure 2D, media measurement of Nluc-GPC4 with and without PI-PLC treatment | Yes | Unpaired t test | Cohen’s d = 3.00, p < 0.0001 |

Figure 2E, overnight release of GPC4 compared with Prion, normalized to lysate | Yes | Unpaired t test | Cohen’s d = 3.59, p < 0.0001 |

Figure 3A, live surface Biotinylation of GPC4 release over time | Yes | ANOVA; Tukey’s multiple comparisons | Cohen’s d = 1.012, p < 0.0001 |

Figure 3B, ultracentrifugation of Nluc-GPC4 in ACMs | Yes | Unpaired t test | Cohen’s d = 7.829, p < 0.0001 |

Figure 3C, pulldown of Nluc-GPC4 by α Toxin | Yes | ANOVA; Tukey’s multiple comparisons | Cohen’s d = 7.659, p < 0.0001 |

Figure 4A, reduction of Nluc-GPC4 by protease inhibitor GM6001 | Yes | Unpaired t test | Cohen’s d = 1.118, p = 0.0003 |

Figure 4B, reduction of Nluc-GPC4 release by Adam9 shRNA1 KD | Yes | ANOVA; Tukey’s multiple comparisons | Cohen’s d = 1.832, p < 0.0001 |

Figure 4B, reduction of Nluc-GPC4 release by Adam9 shRNA2 KD | Yes | ANOVA; Tukey’s multiple comparisons | Cohen’s d = 2.63, p < 0.0001 |

Figure 4C, fold gene expression by qPCR for shRNA KD experiments Adam9 shRNA1 | Yes | One-sample t test | Cohen’s d = 1.393, p = 0.0190 |

Figure 4C, fold gene expression by qPCR for shRNA KD experiments Adam9 shRNA2 | Yes | One-sample t test | Cohen’s d = 0.799, p = 0.1076 |

Figure 4C, fold gene expression by qPCR for shRNA KD experiments Adam10 shRNA1 | Yes | One-sample t test | Cohen’s d = 10.33, p < 0.0001 |

Figure 4C, fold gene expression by qPCR for shRNA KD experiments Adam10 shRNA2 | Yes | One-sample t test | Cohen’s d = 1.089, p = 0.0444 |

Figure 4D, induction of Nluc-GPC4 release by Adam9 Overexpression | Yes | ANOVA; Tukey’s multiple comparisons | Cohen’s d = 3.482, p < 0.0001 |

Figure 5A, heparinase treatment of Nluc-GPC4 vs Control | Yes | One-sample t test | Cohen’s d = 2.93, p = 0.0365 |

Figure 5B, pulldown of Nluc-GPC4 by α Toxin in PI-PLC control vs basal release conditions | Yes | Unpaired t test | Cohen’s d = 0.390, p = 0.9467 |

Extended Data Figure 1-1, cytotoxicity assay of PI-PLC treatment | Yes | ANOVA; Tukey’s multiple comparisons | Cohen’s d = 0.907, p = 0.964 |

Extended Data Figure 1-1, cytotoxicity assay of Edelfosine treatment | Yes | ANOVA; Tukey’s multiple comparisons | Cohen’s d = 2.870, p < 0.0001 |

Extended Data Figure 2-1, lysate measurement of Nluc-GPC4-TM with and without PI-PLC treatment | Yes | Unpaired t test | Cohen’s d = 0.49, p = 0.19 |

Extended Data Figure 2-1, media measurement of Nluc-GPC4 = TM with and without PI-PLC treatment | Yes | Unpaired t test | Cohen’s d = 0.50, p = 0.27 |