Table 1

Primers used

SetFP/RPPrimer sequenceGene nameVector backbone
Set 1FPCGATGGCCGGCCACCATGGCACGCTTAGGCTTGCTCGpc4pCAG GPC4
RPGCACTTCCGAGCAACTTTTC
Set 2FPCCACCATGGCACGCTTAGGCTTGCTCGCGCTCCTCTGCACCCTGGCCGCGCTCAGCGCCTCGCTGCTGGCTGATATCGCGGAGCTGpc4 (endogenous signal peptide)pCAG GPC4
RPCCGCGATATCAGCCAGCAGCGAGGCGCTGAGCGCGGCCAGGGTGCAGAGGAGCGCGAGCAAGCCTAAGCGTGCCATGGTGGCCGG
Set 3FPTCGCTGCTGGCTGATATGGTCTTCACACTCGAAGATTTCGNano luciferasepCAG GPC4
RPCTTGAGCTCCGCGATCTTATCGTCGTCATCCTTGTAATCCGCCAGAATGCGTTCGCACAG
Set 4FPATAGGCTAGCCTCGAGACCATGGCACGCTTAGGCTTGNluc-GPC4pZac2.1 Nluc-GPC4
RPCCGGGTCGACTCTAGATTATCTCCACTCTCCCTGCAC
Set 4ARPATCGTCTAGATCATTACTTGTACAGCTCGTCCATNluc-GPC4TM
Set 5FPATTCTGGCGTCCGGAAAAAAGCGGCCAAAGCCTGGAPrionpZac2.1 Nluc-Prion
RPTCCAGGCTTTGGCCGCTTTTTTCCGGACGCCAGAAT
Set 6FPAATTCGCGCCTAGGTCCGAACCCCGTTCGGACCTAGGCGCGAATTATTTTTshRNA controlpSilencer 1.0 U6
RPAATTAAATTCGCGCCTAGGTCCGAACGGGGTTCGGACCTAGGCGCGAATTGGCC
Set 7FPACAAGAACCACAATTACTAGATCAAGAGTCTAGTAATTGTGGTTCTTGTATTTTTAdam9 shRNA1pSilencer 1.0 U6
RPAATTAAAAATACAAGAACCACAATTACTAGACTCTTGATCTAGTAATTGTGGTTCTTGTGGCC
Set 7AFPGCCAATAACGTCTGCATTTTCAAGAGAAATGCAGACGTTATTGGCATTTTTAdam9 shRNA2pSilencer 1.0 U6
RPAATTAAAAATGCCAATAACGTCTGCATTTTCAAGAGAAATGCAGACGTTATTGGCGGCC
Set 8FPGCAAATGATACCCTTACAGTTTCAAGAGAACTGTAAGGGTATCATTTGCATTTTTAdam10 shRNA1pSilencer 1.0 U6
RPAATTAAAAATGCAAATGATACCCTTACAGTTCTCTTGAAACTGTAAGGGTATCATTTGCGGCC
Set 8AFPGGAAGACAGTTCAACCTACGATCAAGAGTCGTAGGTTGAACTGTCTTCCATTTTTAdam10 shRNA2pSilencer 1.0 U6
RPAATTAAAAATGGAAGACAGTTCAACCTACGACTCTTGATCGTAGGTTGAACTGTCTCCGGCC
Set 9FPGGGGTGTGTAACAGCAATAAGADAM9Sequencing primers
RPCAAGGGGGACGATTAGGAAG
Set 10FPAACAGGCTTATCGCTATGADAM10Sequencing primers
RPCTGCTGCTGACTTCTAAT
Set 11FPCTGGAGAAACCTGCCAAGTAGAPDHSequencing primers
RPAGTGGGAGTTGCTGTTGAAG