Table 1

Primers used

Set	FP/RP	Primer sequence	Gene name	Vector backbone
Set 1	FP	CGATGGCCGGCCACCATGGCACGCTTAGGCTTGCTC	Gpc4	pCAG GPC4
Set 1	RP	GCACTTCCGAGCAACTTTTC
Set 2	FP	CCACCATGGCACGCTTAGGCTTGCTCGCGCTCCTCTGCACCCTGGCCGCGCTCAGCGCCTCGCTGCTGGCTGATATCGCGGAGCT	Gpc4 (endogenous signal peptide)	pCAG GPC4
Set 2	RP	CCGCGATATCAGCCAGCAGCGAGGCGCTGAGCGCGGCCAGGGTGCAGAGGAGCGCGAGCAAGCCTAAGCGTGCCATGGTGGCCGG
Set 3	FP	TCGCTGCTGGCTGATATGGTCTTCACACTCGAAGATTTCG	Nano luciferase	pCAG GPC4
Set 3	RP	CTTGAGCTCCGCGATCTTATCGTCGTCATCCTTGTAATCCGCCAGAATGCGTTCGCACAG
Set 4	FP	ATAGGCTAGCCTCGAGACCATGGCACGCTTAGGCTTG	Nluc-GPC4	pZac2.1 Nluc-GPC4
Set 4	RP	CCGGGTCGACTCTAGATTATCTCCACTCTCCCTGCAC		pZac2.1 Nluc-GPC4
Set 4A	RP	ATCGTCTAGATCATTACTTGTACAGCTCGTCCAT	Nluc-GPC4^TM
Set 5	FP	ATTCTGGCGTCCGGAAAAAAGCGGCCAAAGCCTGGA	Prion	pZac2.1 Nluc-Prion
Set 5	RP	TCCAGGCTTTGGCCGCTTTTTTCCGGACGCCAGAAT
Set 6	FP	AATTCGCGCCTAGGTCCGAACCCCGTTCGGACCTAGGCGCGAATTATTTTT	shRNA control	pSilencer 1.0 U6
Set 6	RP	AATTAAATTCGCGCCTAGGTCCGAACGGGGTTCGGACCTAGGCGCGAATTGGCC
Set 7	FP	ACAAGAACCACAATTACTAGATCAAGAGTCTAGTAATTGTGGTTCTTGTATTTTT	Adam9 shRNA1	pSilencer 1.0 U6
Set 7	RP	AATTAAAAATACAAGAACCACAATTACTAGACTCTTGATCTAGTAATTGTGGTTCTTGTGGCC
Set 7A	FP	GCCAATAACGTCTGCATTTTCAAGAGAAATGCAGACGTTATTGGCATTTTT	Adam9 shRNA2	pSilencer 1.0 U6
Set 7A	RP	AATTAAAAATGCCAATAACGTCTGCATTTTCAAGAGAAATGCAGACGTTATTGGCGGCC
Set 8	FP	GCAAATGATACCCTTACAGTTTCAAGAGAACTGTAAGGGTATCATTTGCATTTTT	Adam10 shRNA1	pSilencer 1.0 U6
Set 8	RP	AATTAAAAATGCAAATGATACCCTTACAGTTCTCTTGAAACTGTAAGGGTATCATTTGCGGCC
Set 8A	FP	GGAAGACAGTTCAACCTACGATCAAGAGTCGTAGGTTGAACTGTCTTCCATTTTT	Adam10 shRNA2	pSilencer 1.0 U6
Set 8A	RP	AATTAAAAATGGAAGACAGTTCAACCTACGACTCTTGATCGTAGGTTGAACTGTCTCCGGCC
Set 9	FP	GGGGTGTGTAACAGCAATAAG	ADAM9	Sequencing primers
Set 9	RP	CAAGGGGGACGATTAGGAAG
Set 10	FP	AACAGGCTTATCGCTATG	ADAM10	Sequencing primers
Set 10	RP	CTGCTGCTGACTTCTAAT
Set 11	FP	CTGGAGAAACCTGCCAAGTA	GAPDH	Sequencing primers
Set 11	RP	AGTGGGAGTTGCTGTTGAAG