At each hierarchal level (Lvl) of the AMB, the agreement between a blinded rater allocating microspheres from histologic images and our block-face imaging workflow (upper) or compared with registering histologic sections with QuickNII (lower)
| Category | Lvl 10 | Lvl 9 | Lvl 8 | Lvl 7 | Lvl 6 | Lvl 5 | Lvl 3/4 | Lvl 1/2 | |
|---|---|---|---|---|---|---|---|---|---|
| Manual histology compared with block-face imaging workflow | Agreement | 18 (34.6%) | 29 (55.8%) | 39 (75%) | 45 (86.5%) | 50 (96.2%) | 50 (96.2%) | 50 (96.2%) | 51 (98.1%) |
| Microsphere not at this level | 27 (51.9%) | 16 (30.8%) | 8 (15.4%) | 4 (7.7%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | |
| Sum of agreement | 45 (86.5%) | 45 (86.5%) | 47 (90.4%) | 49 (94.2%) | 50 (96.2%) | 50 (96.2%) | 50 (96.2%) | 51 (98.1%) | |
| Disagreement | 7 (13.5%) | 7 (13.5%) | 5 (9.6%) | 3 (5.8%) | 2 (3.8%) | 2 (3.8%) | 2 (3.8%) | 1 (1.9%) | |
| Total | 52 | 52 | 52 | 52 | 52 | 52 | 52 | 52 | |
| Manual histology compared with registering Histologic sections with QuickNII | Agreement | 22 (42.3%) | 30 (57.7%) | 40 (76.9%) | 45 (86.5%) | 48 (92.3%) | 49 (94.2%) | 50 (96.2%) | 51 (98.1%) |
| Microsphere not at this level | 20 (38.5%) | 14 (26.9%) | 4 (7.7%) | 1 (1.9%) | 0 (0%) | 0 (0%) | 0 (0%) | 0 (0%) | |
| Sum of agreement | 42 (80.7%) | 44 (84.6%) | 44 (84.6%) | 46 (88.5%) | 48 (92.3%) | 49 (94.2%) | 50 (96.2%) | 51 (98.1%) | |
| Disagreement | 10 (19.2%) | 8 (15.4%) | 8 (15.4%) | 6 (11.5%) | 4 (7.7%) | 3 (5.8%) | 2 (3.8%) | 1 (1.9%) | |
| Total | 52 | 52 | 52 | 52 | 52 | 52 | 52 | 52 |
Agreement represents the number of microsphere given the same allocation between the rater and either workflow. Microsphere not at this level, represents the number of microspheres not located at a given level of the hierarchal tree. Some microspheres are not located at specific levels of the hierarchal tree, since every subregion of the AMB hierarchal tree does not end at the same level. Sum of agreement, the number of microspheres correctly allocated up to the specific hierarchal level. Disagreement, represents the number of microspheres incorrectly allocated at the given level. With 94.2% agreement, we are confident we can allocate microspheres from block-face imaging to level 7 of the hierarchal tree of the AMB atlas (e.g., somatomotor, somatosensory, visual, piriform areas, etc.). In contrast, registering histologic sections with QuickNII, rather than block-face images, results in 88.5% agreement at level 7.