Agreement between manual allocation of individual microspheres from block-face images and our block-face imaging workflow
| Brain region | ||||||||
|---|---|---|---|---|---|---|---|---|
| Category | Total counts | Olfactory aeas | Isocortex | Corpus callosum | Hippocampal region | Striatum | Thalamus and midbrain | Other |
| Agreement | 995 (93.9%) | 68 (88.3%) | 383 (94.6%) | 33 (75%) | 129 (96.3% | 96 (92.3%) | 261 (97%) | 25 (92.6%) |
| Disagreement | 65 (6.1%) | 9 (11.7%) | 22 (4.4%) | 11 (25%) | 5 (3.7%) | 8 (7.5%) | 8 (3%) | 2 (7.4%) |
| Total | 1061 | 77 (7.3%) | 405 (38.2%) | 44 (4.2%) | 134 (12.6%) | 104 (9.8%) | 269 (25.4%) | 27 (2.5%) |
Agreement represents the same allocation between our workflow and manual rating. Overall, there is 93.9% agreement between our workflow and a human rater (N = 1061). Microspheres were predominantly found in isocortex and thalamus/midbrain. Data are presented as number of microspheres found in a given region, with the percentage relative to the total counts column found in parenthesis.