Table 2

Statistical summary and analysis methods

Figure reported	N, # rats	n, # cells	Norm. dist?*	Statistic	Statistic value (df)	p value	Variance source	Post hoc test	Post hoc p	Mean difference	Lower 95% CI	Upper 95% CI
1D, mCherry vs GFP	6	N/A	N/A	Unpaired t test	t₍₁₀₎ = 3.75	0.0038	Difference			36.4	14.75	58.04

3A, eYFP vs No eYFP (current inject vs eYFP)	11	23	Yes	Two-way RM-ANOVA	F_(11,231) = 30.23	<0.0001	Interaction			55.91	34.80	77.00
3A, eYFP+ vs eYFP (current inject)	11	23	Yes	Two-way RM-ANOVA	F_(11,231) = 51.63	<0.0001	Main effect
3A, eYFP+ vs eYFP (eYFP)	11	23	Yes	Two-way RM-ANOVA	F_(1,21) = 17.61	0.00040	Main effect
3D, eYFP+ vs eYFP (max current)	4	15	Yes	Unpaired t test	t₍₂₂₎ = 5.804	<0.0001	Difference			82.03	55.61	108.50
4B, control	6	14	Yes
4B, gabazine	6	14	Yes	Paired t test	t₍₁₃₎ = 6.485	<0.0001	Difference			−101.90	−135.80	−67.95
5A, R_in (data not shown	11	28	Yes	Unpaired t test	t₍₂₁₎ = 1.25	0.2250	Difference			102.60	98.25	107.00
5A,B, control	11	22		Unpaired t test	t₍₈₀₎ = 0.38	0.0970	Difference			−0.104	−5.46	5.25
5C, outward I	11	4		One-sample test	t₍₄₀₎ = 7.94	<0.0001	Difference			9.20	6.86	11.50
5D, ketanserin, male vs female	8	16	Yes	Two-way ANOVA	F_(1,27) = 8.671	0.0066	Interaction
5D, ketanserin, male vs female	8	16		Two-way ANOVA	F_(1,27) = 8.135	0.0082	Main effect (sex)
5D, ketanserin, male vs female	8	16		Two-way ANOVA	F_(1,27) = 3.694	0.0652	Main effect (treatment)
5D, ketanserin, male vs female	8	16					Male vs female 5-HT	Tukey	0.880	5.30	−14.31	24.92
5D, ketanserin, male vs female	8	16					Male 5-HT vs +ketanserin	Tukey	>0.999	0.48	−21.17	22.13
5D, ketanserin, male vs female	8	16					Female 5-HT vs +ketanserin	Tukey	0.0007	−30.06	−48.41	−11.71
5D, ketanserin, male vs female	8	16					Female ketanserin vs Male ketanserin	Tukey	0.0116	−25.24	−45.75	−4.72
6Bz, sEPSC interevent male vs female	8	17	Yes	Kolmogorov–Smirnov	0.6455	<0.0001	Difference			177.7	168.00	187.30
6D, sEPSC frequency male vs female and 5-HT	8	17	Yes	Two-way ANOVA	F_(1,30) = 0.0402	0.842	Interaction
6D, sEPSC frequency male vs female	8	17	Yes	Two-way ANOVA	F_(1,30) = 20.52	<0.0001	Main effect (sex)
6D, sEPSC frequency 5-HT	8	17	Yes	Two-way ANOVA	F_(1,30) = 0.1990	0.6590	Main effect (5-HT)
6D, sEPSC frequency male vs female	8	17					Male vs Female baseline sEPSC	Tukey	0.011	12.31	2.30	22.32
6D, sEPSC frequency male vs female and 5-HT	8	17					Male baseline vs male 5-HT	Tukey	0.970	1.68	−8.62	11.98
6D, sEPSC frequency male vs female and 5-HT	8	17					Female baseline vs female 5-HT	Tukey	0.998	0.64	−9.07	10.35
6E, sEPSC amplitude 5-HT	8	17	Yes	Two-way ANOVA	F_(1,30) = 0.0016	0.9680	Interaction
6E, sEPSC amplitude 5-HT	8	17	Yes	Two-way ANOVA	F_(1,30) = 0.0085	0.9270	Main effect (sex)
6ED, sEPSC amplitude 5-HT	8	17	Yes	Two-way ANOVA	F_(1,30) = 0.0054	0.9418	Main effect (5-HT)
7B, 10-Hz train	8	21	N/A	Two-way ANOVA	F_(1,20) = 6.100	0.0226	Treatment			8.86	−26.95	44.67
7B, 20-Hz train	8	19	N/A	Two-way ANOVA	F_(1,18) = 4.618	0.0455	Treatment			3.97	−36.12	44.06
8B, total cocaine intake male-female	17	N/A		Unpaired t test	t₍₂₂₎ = 1.463	0.1577	Difference			4.89	−2.04	11.82
8C, CSA, RMP	19	45	Yes	Two-way ANOVA	F_(1,35) = 13.26	0.0009	Interaction
8C, CSA, RMP	19	45			F_(1,35) = 2.279	0.1401	Main effect (sex)
8C, CSA, RMP	19	45			F_(1,35) = 4.459	0.0419	Main effect (treatment-CSA)
8C, CSA, RMP	19	45	Yes				Naive male vs naive female	Tukey	0.520	−4.50	−13.30	4.30
8C, CSA, RMP	19	45	Yes				Naive male vs CSA male	Tukey	0.0015	−12.15	−20.26	−4.03
8C, CSA, RMP	19	45					CSA male vs CSA female	Tukey	0.0014	10.88	3.646	18.10
8C, CSA, RMP	19	45					Naive female vs CSA female	Tukey	0.698	3.23	−4.763	11.22
8D, CSA, I-Hold	30	72	Yes	Two-way ANOVA	F_(1,68) = 9.115	0.0036	Interaction
8D, CSA, I-Hold	30	72			F_(1,68) = 0.0036	0.9521	Main effect (sex)
8D , CSA, I-Hold	30	72			F_(1,68) = 7.145	0.0094	Main effect (treatment-CSA)
8D, CSA, I-Hold	30	72					naive male vs naive female	Tukey	0.261	35.51	−15.164	86.19
8D, CSA, I-Hold	30	72					Naive male vs CSA male	Tukey	<0.0001	68.32	29.495	107.15
8D, CSA, I-Hold	30	72					Naive female vs CSA female	Tukey	0.996	−4.15	−54.050	45.74
8D, CSA, I-Hold	30	72					CSA male vs CSA female	Tukey	0.057	−36.96	−74.761	0.84
8E, firing, male CSA	8	17	Yes	One-way RM-ANOVA	F_(7,21) = 1.288	0.3047	Treatment			−2.421	−23.500	18.70
8F, firing, female CSA	11	24	Yes	One-way RM-ANOVA	F_(7,21) = 1.288	0.3047	Treatment			6.67	−9.047	22.36
8G, Ihold 5-HT, CSA, male	16	39	N/A	Unpaired t test	t₍₃₈₎ = 0.6226	0.5373	Treatment			−1.625	−6.909	3.66
8H, Ihold 5-HT, CSA, female	14	30	N/A	Unpaired t test	t₍₃₈₎ = 0.0742	0.9412	Treatment			−0.290	−8.154	7.58
8, data (male female proportions)	30	66	N/A	Fisher’s	N/A	0.7021	Treatment			N/A	N/A	N/A
8 data (male female days CSA withdrawal)	30	66	Yes	Unpaired t test	t₍₁₇₎ = 1.733	0.1013	Sex			18.82	−4.098	41.74
9B, sEPSC frequency male vs female CSA	19	43	Yes	Two-way ANOVA	F_(1,39) = 12.05	0.0013	Interaction
9B, sEPSC frequency male vs female CSA	19	43			F_(1,39) = 4.434	0.0417	Main effect (sex)
9B, sEPSC frequency male vs female CSA	19	43			F_(1,39) = 2.057	0.1595	Main effect (treatment-CSA)
9B, sEPSC frequency male vs female CSA	19	43					Naive male vs naive female	Tukey	0.0047	12.31	3.137	21.49
9B, sEPSC frequency male vs female CSA	19	43					Naive male vs CSA male	Tukey	0.006	10.83	2.563	19.10
9B, sEPSC frequency male vs female CSA	19	43					Naive female vs CSA female	Tukey	0.494	−4.50	−12.984	3.99
9B, sEPSC frequency male vs female CSA	19	43					CSA male vs CSA female	Tukey	0.704	−3.02	−10.510	4.48
9C, sEPSC amplitude male vs female CSA	19	42	Yes	Two-way ANOVA	F_(1,38) = 0.0691	0.8018	Interaction
9C, sEPSC amplitude male vs female CSA	19	42			F_(1,38) = 0.1930	0.6629	Main effect (sex)
9C, sEPSC amplitude male vs female CSA	19	42			F_(1,38) = 1.998	0.1657	Main effect (treatment-CSA)
9D, sEPSC frequency male vs female CSA, 5-HT	19	42	Yes	Two-way ANOVA	F_(1,38) = 0.5817	0.4503	Interaction
9D, sEPSC frequency male vs female CSA, 5-HT	19	42			F_(1,38) = 0.3550	0.5548	Main effect (sex)
9D, sEPSC frequency male vs female CSA, 5-HT	19	42			F_(1,38) = 0.095	0.7600	Main effect (treatment-CSA)

* D’Agostino–Pearson test of normality.