Table 2

Secondary antibodies

Secondary antibodies
AntibodyHost, isotypeImmunogenSourceCatalog numberClone no./RRIDDilution
Anti-rabbit IgG, HRP conjugateGoat, IgGIgG rabbitCell Signaling Technology7074P2RRID:AB_20992331:7500
Anti-mouse IgG, HRP conjugateGoat, IgGIgG mouseThermo Fisher ScientificA16072RRID:AB_25347451:5000
Anti-goat IgG, HRP conjugateDonkey, IgGIgG goatThermo Fisher ScientificA15999RRID:AB_25346731:5000
Anti-mouse Alexa Fluor 488Donkey, IgGIgG (H + L) mouseThermo Fisher ScientificA21202RRID:AB_1416071:1000
Anti-mouse Alexa Fluor 647Donkey, IgGIgG (H + L) mouseThermo Fisher ScientificA31571RRID:AB_1625421:1000
Anti-rabbit Alexa Fluor 488Donkey, IgGIgG (H + L) rabbitThermo Fisher ScientificA21206RRID:AB_25357921:1000
Anti-rabbit Alexa Fluor 568Donkey, IgGIgG (H + L) rabbitThermo Fisher ScientificA10042RRID:AB_25340171:1000
Anti-rabbit Alexa Fluor 647Donkey, IgGIgG (H + L) rabbitThermo Fisher ScientificA31573RRID:AB_25361831:1000
Anti-guinea pig Alexa Fluor 488Donkey, IgGIgG (H + L) guinea pigJackson ImmunoResearch706545148RRID:AB_23404721:1000
Anti-guinea pig Alexa Fluor 568Goat, IgGIgG (H + L) guinea pigThermo Fisher ScientificA11075RRID:AB_25341191:1000

  • List of secondary antibodies used for Western blotting and immunocytochemistry. HRP, horseradish peroxidase.