Primary antibodies
| Primary antibodies | ||||||
|---|---|---|---|---|---|---|
| Antibody | Host, isotype | Immunogen | Source | Catalog number | Clone no./RRID | Dilution |
| Cadherin-8 | Mouse | N terminus, mouse | DSHB | CAD8-1 | RRID:AB_2078272 | 0.5 μg/ml (WB), 3–4 μg/ml (ICC), 2 μg (IP) |
| Cadherin-8 | Goat, IgG | C terminus, human | Santa Cruz | sc-6461 | C-18/RRID:AB_2078271 | 1:500 |
| Cadherin-11 | Mouse, IgG1 | C terminus, human | Thermo Fisher Scientific | 32-1700 | 5B2H5/RRID:AB_2533068 | 2 μg/ml (WB), 7 μg (IP) |
| PSD-95 | Mouse, IgG2a | Amino acids 77–299, human | Neuro Mab | 75-028 | K28/43/RRID:AB_2307331 | 1:100,000 (WB) |
| PSD-95 | Guinea pig, IgG | Amino acids 64–247, mouse | Synaptic Systems | 124014 | RRID:AB_2619800 | 1:500 (ICC) |
| Syntaxin-1 | Mouse, IgG2a | N terminus, rat | Synaptic Systems | 110111 | 78.3/RRID:AB_887848 | 1:10,000 |
| Synapsin-1 | Rabbit, IgG | Native protein purified from bovine brain | Abgent | P17599 | N/A | 1:1000 |
| GAT-1 | Rabbit, IgG | C terminus, rat | Phospho Solutions | 880-GAT1 | RRID:AB_2492119 | 1:200 |
| Neuroligin-1 | Mouse, IgG1 | Amino acids 718–843, rat | Neuro Mab | 75-160 | N97A/31/RRID:AB_2235964 | 1:100 |
| N-cadherin | Mouse, IgG1 | Amino acids 802–819, mouse | BD Transduction Labs | 610920 | 32/RRID:AB_2077527 | 1:1000 |
| β-Catenin | Mouse, IgG1 | Amino acids 571–781, mouse | BD Transduction Labs | 610153 | 14/RRID:AB_397554 | 1:700 |
| GAPDH | Rabbit, IgG | C terminus, human | Cell Signaling Technology | 5174 | D16H11/RRID:AB_10622025 | 1:1000 |
| β-Actin | Mouse, IgG2a | N terminus | Millipore Sigma | A5441 | AC-74/RRID:AB_476743 | 1:5000 |
| HA | Rabbit, IgG | Amino acids 98–106 of human influenza hemagglutinin | Millipore Sigma | H6908 | RRID:AB_260070 | 1:1000 (WB), 1:25 (ICC), 4 μg (IP) |
| C-myc | Mouse, IgG1 | Amino acids 408–439 of human p62c-myc | Millipore Sigma | M4439 | 9E10/RRID:AB_291324 | 1:3000 (WB), 1:500 (ICC) |
| Flag | Mouse, IgG2b | DYKDDDDK tag | Thermo Fisher Scientific | MA191878 | FG4R/RRID:AB_1957945 | 1:500 (ICC) |
List of primary antibodies used for Western blotting (WB), immunocytochemistry (ICC), and immunoprecipitation (IP). PSD-95, postsynaptic density protein-95; GAT-1, sodium-dependent and chloride-dependent GABA transporter-1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; aa, amino acid.