Summary of statistical analyses
| Figure | Measurement | Type of test | Comparison | Statistical value |
|---|---|---|---|---|
| 2G | Number of turns during free swimming | Welch’s t test | Learner WT vs non-learner WT | p = 0.5924 t(41) = 0.5397 |
| 2H | Swimming speed during free swimming | Welch’s t test | Learner WT vs non-learner WT | p = 0.4405 t(41) = 0.7791 |
| 2J | Swimming speed before and after electric shock | Welch’s t test | Before US vs after US in WT | p = 0.002975 t(12) = −4.539 |
| 3A | Number of turns during free swimming | Welch’s t test | Control vs 152B::BoTx | p = 0.6224 t(86) = −0.4942 |
| 3B | Swimming speed during free swimming | Welch’s t test | Control vs 152B::BoTx | p = 0.7603 t(86) = −0.3062 |
| 3C | Number of turns during free swimming | Welch’s t test | Control vs cbln12::BoTx | p = 0.03522 t(75) = −2.145 |
| 3D | Swimming speed during free swimming | Welch’s t test | Control vs cbln12::BoTx | p = 0.3511 t(75) = −0.9397 |
| 3E | Swimming speed before and after electric shock | Welch’s t test | Before US vs after US in 152B::BoTx | p = 4.043e-05 t(12) = −6.314 |
| 3F | Swimming speed before and after electric shock | Welch’s t test | Before US vs after US in cbln12::BoTx | p = 0.01427 t(12) = −3.130 |
| 3G | Learning rate of active avoidance | Fisher’s exact test | Control vs 152B::BoTx | p = 5.574e-08 |
| 3H | Learning rate of active avoidance | Fisher’s exact test | Control vs cbln12::BoTx | p = 0.01080 |
| 4A | Number of turns during free swimming | Welch’s t test | Control vs aldoca:BoTx | p = 0.2164 t(78) = 1.246 |
| 4B | Swimming speed during free swimming | Welch’s t test | Control vs aldoca:BoTx | p = 0.6001 t(78) = −0.5264 |
| 4C | Swimming speed before and after electric shock | Welch’s t test | before US vs after US in aldoca:BoTx | p = 0.0001143 t(12) = −6.248 |
| 4D | Learning rate of active avoidance | Fisher’s exact test | Control vs aldoca:BoTx | p = 0.002116 |
| 4E | Number of trials in training session 1 | Welch’s t test | Control vs aldoca:BoTx | p = 0.3255 t(20) = −1.031 |
| 4E | Number of trials in training session 2 | Welch’s t test | Control vs aldoca:BoTx | p = 0.7545 t(20) = 0.3182 |
| 4E | Number of trials in training session 3 | Welch’s t test | Control vs aldoca:BoTx | p = 0.3865 t(20) = 0.8856 |
| 4E | Number of trials in test session | Welch’s t test | Control vs aldoca:BoTx | p = 0.2301 t(20) = −1.360 |
| 5M | Number of turns during free swimming | Welch’s t test | Control vs aldoca:NTR | p = 0.2728 t(54) = −1.143 |
| 5N | Swimming speed during free swimming | Welch’s t test | Control vs aldoca:NTR | p = 0.02462 t(54) = 2.509 |
| 5O | Swimming speed before and after electric shock | Welch’s t test | Before US vs after US in aldoca:NTR | p = 0.0003567 t(12) = −6.371 |
| 5P | Swimming speed before and after electric shock | One-way ANOVA | F(18,621) = 2.88 p = 0.03943 | |
| 5P | Swimming speed before and after electric shock | One-way ANOVA with Tukey’s post hoc test | WT vs 152B::BoTx | p = 0.6201 |
| 5P | Swimming speed before and after electric shock | One-way ANOVA with Tukey’s post hoc test | WT vs cbln12::BoTx | p = 0.08734 |
| 5P | Swimming speed before and after electric shock | One-way ANOVA with Tukey’s post hoc test | WT vs aldoca:BoTx | p = 0.7949 |
| 5P | Swimming speed before and after electric shock | One-way ANOVA with Tukey’s post hoc test | WT vs aldoca:BoTx | p = 0.9959 |
| 5Q | Learning rate of active avoidance | Fisher’s exact test | Control vs aldoca:NTR | p = 0.008326 |
| 6B | Change of speed in training session | Two-way repeated measures ANOVA lines × trials interaction | F(18,621) = 3.515 p = 1.398e-06 | |
| 6B | Change of speed in training session | Two-way repeated measures ANOVA lines factor | F(2,69) = 19.92 p = 1.483e-07 | |
| 6B | Change of speed in training session | Two-way repeated measures ANOVA trials factor | F(9,621) = 5.725 p = 1.189e-07 | |
| 6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoTx in trial 6 | p = 0.03243 |
| 6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoT in trial 7 | p = 0.04779 |
| 6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoT in trial 8 | p = 4.1e-09 |
| 6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs aldoca:BoTx in trial 8 | p = 2.6e-05 |
| 6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoTx in trial 9 | p = 0.005936 |
| 6B | Change of speed in test session | Two-way repeated measures ANOVA lines × trials interaction | F(18,621) = 1.083 p = 0.3649 | |
| 6B | Change of speed in test session | Two-way repeated measures ANOVA lines factor | F(2,69) = 28.35 p = 1.398e-06 | |
| 6B | Change of speed in test session | Two-way repeated measures ANOVA trials factor | F(9,621) = 1.431 p = 0.171 | |
| 6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoTx | p < 1e-22 |
| 6B | Change of speed in training session | One-way ANOVA with Tukey’s post hoc test | WT vs aldoca:BoTx | p < 1e-22 |
| 6B | Change of speed in training session | One-way ANOVA with Tukey’s post hoc test | aldoca:BoTx vs 152B::BoTx | p = 0.9425 |
| 6C | Learning rate of Pavlovian fear conditioning | Fisher’s exact test with BH post hoc test | WT vs 152B::BoTx | p = 0.02892 |
| 6C | Learning rate of Pavlovian fear onditioning | Fisher’s exact test with BH post hoc test | WT vs aldoca:BoTx | p = 0.02892 |
| 6C | Learning rate of Pavlovian fear conditioning | Fisher’s exact test with BH post hoc test | 152B::BoTx vs aldoca:BoTx | p = 1.000 |
In all figures, the data distribution was normal. WT, wild-type.