Figure | Measurement | Type of test | Comparison | Statistical value |
---|---|---|---|---|

2G | Number of turns during free swimming | Welch’s t test | Learner WT vs non-learner WT | p = 0.5924t_{(41)} = 0.5397 |

2H | Swimming speed during free swimming | Welch’s t test | Learner WT vs non-learner WT | p = 0.4405t_{(41)} = 0.7791 |

2J | Swimming speed before and after electric shock | Welch’s t test | Before US vs after US in WT | p = 0.002975t_{(12)} = −4.539 |

3A | Number of turns during free swimming | Welch’s t test | Control vs 152B::BoTx | p = 0.6224t_{(86)} = −0.4942 |

3B | Swimming speed during free swimming | Welch’s t test | Control vs 152B::BoTx | p = 0.7603t_{(86)} = −0.3062 |

3C | Number of turns during free swimming | Welch’s t test | Control vs cbln12::BoTx | p = 0.03522t_{(75)} = −2.145 |

3D | Swimming speed during free swimming | Welch’s t test | Control vs cbln12::BoTx | p = 0.3511t_{(75)} = −0.9397 |

3E | Swimming speed before and after electric shock | Welch’s t test | Before US vs after US in 152B::BoTx | p = 4.043e-05t_{(12)} = −6.314 |

3F | Swimming speed before and after electric shock | Welch’s t test | Before US vs after US in cbln12::BoTx | p = 0.01427t_{(12)} = −3.130 |

3G | Learning rate of active avoidance | Fisher’s exact test | Control vs 152B::BoTx | p = 5.574e-08 |

3H | Learning rate of active avoidance | Fisher’s exact test | Control vs cbln12::BoTx | p = 0.01080 |

4A | Number of turns during free swimming | Welch’s t test | Control vs aldoca:BoTx | p = 0.2164t_{(78)} = 1.246 |

4B | Swimming speed during free swimming | Welch’s t test | Control vs aldoca:BoTx | p = 0.6001t_{(78)} = −0.5264 |

4C | Swimming speed before and after electric shock | Welch’s t test | before US vs after US in aldoca:BoTx | p = 0.0001143t_{(12)} = −6.248 |

4D | Learning rate of active avoidance | Fisher’s exact test | Control vs aldoca:BoTx | p = 0.002116 |

4E | Number of trials in training session 1 | Welch’s t test | Control vs aldoca:BoTx | p = 0.3255t_{(20)} = −1.031 |

4E | Number of trials in training session 2 | Welch’s t test | Control vs aldoca:BoTx | p = 0.7545t_{(20)} = 0.3182 |

4E | Number of trials in training session 3 | Welch’s t test | Control vs aldoca:BoTx | p = 0.3865t_{(20)} = 0.8856 |

4E | Number of trials in test session | Welch’s t test | Control vs aldoca:BoTx | p = 0.2301t_{(20)} = −1.360 |

5M | Number of turns during free swimming | Welch’s t test | Control vs aldoca:NTR | p = 0.2728t_{(54)} = −1.143 |

5N | Swimming speed during free swimming | Welch’s t test | Control vs aldoca:NTR | p = 0.02462t_{(54)} = 2.509 |

5O | Swimming speed before and after electric shock | Welch’s t test | Before US vs after US in aldoca:NTR | p = 0.0003567t_{(12)} = −6.371 |

5P | Swimming speed before and after electric shock | One-way ANOVA | F_{(18,621)} = 2.88p = 0.03943 | |

5P | Swimming speed before and after electric shock | One-way ANOVA with Tukey’spost hoc test | WT vs 152B::BoTx | p = 0.6201 |

5P | Swimming speed before and after electric shock | One-way ANOVA with Tukey’spost hoc test | WT vs cbln12::BoTx | p = 0.08734 |

5P | Swimming speed before and after electric shock | One-way ANOVA with Tukey’spost hoc test | WT vs aldoca:BoTx | p = 0.7949 |

5P | Swimming speed before and after electric shock | One-way ANOVA with Tukey’spost hoc test | WT vs aldoca:BoTx | p = 0.9959 |

5Q | Learning rate of active avoidance | Fisher’s exact test | Control vs aldoca:NTR | p = 0.008326 |

6B | Change of speed in training session | Two-way repeated measures ANOVA lines × trials interaction | F_{(18,621)} = 3.515p = 1.398e-06 | |

6B | Change of speed in training session | Two-way repeated measures ANOVA lines factor | F_{(2,69)} = 19.92p = 1.483e-07 | |

6B | Change of speed in training session | Two-way repeated measures ANOVA trials factor | F_{(9,621)} = 5.725p = 1.189e-07 | |

6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoTx in trial 6 | p = 0.03243 |

6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoT in trial 7 | p = 0.04779 |

6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoT in trial 8 | p = 4.1e-09 |

6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs aldoca:BoTx in trial 8 | p = 2.6e-05 |

6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoTx in trial 9 | p = 0.005936 |

6B | Change of speed in test session | Two-way repeated measures ANOVA lines × trials interaction | F_{(18,621)} = 1.083p = 0.3649 | |

6B | Change of speed in test session | Two-way repeated measures ANOVA lines factor | F_{(2,69)} = 28.35p = 1.398e-06 | |

6B | Change of speed in test session | Two-way repeated measures ANOVA trials factor | F_{(9,621)} = 1.431p = 0.171 | |

6B | Change of speed in training session | Two-way repeated measures ANOVA with Tukey’s post hoc test | WT vs 152B::BoTx | p < 1e-22 |

6B | Change of speed in training session | One-way ANOVA with Tukey’s post hoc test | WT vs aldoca:BoTx | p < 1e-22 |

6B | Change of speed in training session | One-way ANOVA with Tukey’spost hoc test | aldoca:BoTx vs 152B::BoTx | p = 0.9425 |

6C | Learning rate of Pavlovian fear conditioning | Fisher’s exact test with BHpost hoc test | WT vs 152B::BoTx | p = 0.02892 |

6C | Learning rate of Pavlovian fear onditioning | Fisher’s exact test with BHpost hoc test | WT vs aldoca:BoTx | p = 0.02892 |

6C | Learning rate of Pavlovian fear conditioning | Fisher’s exact test with BH post hoc test | 152B::BoTx vs aldoca:BoTx | p = 1.000 |

In all figures, the data distribution was normal. WT, wild-type.