Baseline | 78 ± 9 | | | Superficial | Deep | | | Superficial | Deep |
E + 20 | 26 ± 3 | | Baseline | 1.80 ± 0.61 | 1.24 ± 0.31 | | Sham (spike/s) | 5.76 ± 4.71 | 6.44 ± 4.22 |
E + 120 | 34 ± 3 | | E + 20 | 0.16 ± 0.04 | 0.26 ± 0.06 | | Eunucleated | 4.56 ± 3.63 | 6.9 ± 4.91 |
| | | E + 60 | 0.42 ± 0.19 | 0.48 ± 0.08 | | Sham (Cont.) | 0.73 ± 0.22 | 0.87 ± 0.14 |
| | | E + 120 | 0.50 ± 0.29 | 0.53 ± 0.18 | | Enucleated | 0.64 ± 0.25 | 0.78 ± 0.17 |
| | | | | | | | | |
Figure 3B | log10(spikes per second) | | | | | | | | |
Superficial | P8–P9 | P10 | P11 | P12 | P13 | P14 | P15 | P16–P17 | ANOVA |
Sham | −0.43 ± 0.49 | −0.03 ± 0.34 | 0.16 ± 0.40 | 0.25 ± 0.19 | 0.29 ± 0.22 | 0.66 ± 0.26 | 0.76 ± 0.22 | 0.87 ± 0.13 | Age F = 63.5 p < 10−10 |
Enucleated | −0.37 ± 0.36 | −0.18 ± 0.16 | −0.10 ± 0.05 | 0.40 ± 0.14 | 0.52 ± 0.19 | 0.49 ± 0.19 | 0.73 ± 0.24 | 1.07 ± 0.10 | Group F = 1.18 p = 0.30 |
| | | | | | | | | Layer F = 1.57 p = 0.21 |
Deep | | | | | | | | | A × G F = 2.03 p = 0.06 |
Sham | −0.51 ± 0.29 | −0.02 ± 0.25 | 0.24 ± 0.33 | 0.33 ± 0.26 | 0.55 ± 0.44 | 0.87 ± 0.18 | 0.10 ± 0.07 | 0.95 ± 0.18 | A × L F = 0.96 p = 0.47 |
Enucleated | −0.53 ± 0.32 | −0.12 ± 0.29 | −0.06 ± 0.15 | 0.40 ± 0.40 | 0.76 ± 0.32 | 0.61 ± 0.23 | 0.61 ± 0.20 | 0.90 ± 0.09 | G × L F = 1.63 p = 0.20 |
| | | | | | | | | A × G × L F = 0.26 p = 0.97 |
| | | | | | | | | |
Figure 3C | Continuity | | | | | | | | |
Superficial | P8–P9 | P10 | P11 | P12 | P13 | P14 | P15 | P16–P17 | |
Sham | 0.11 ± 0.06 | 0.19 ± 0.05 | 0.24 ± 0.13 | 0.40 ± 0.20 | 0.47 ± 0.12 | 0.77 ± 0.19 | 0.78 ± 0.15 | 0.74 ± 0.19 | Age F = 61.47 p < 10−10 |
Enucleated | 0.09 ± 0.01 | 0.13 ± 0.04 | 0.17 ± 0.04 | 0.45 ± 0.11 | 0.41 ± 0.05 | 0.36 ± 0.09 | 0.41 ± 0.16 | 0.70 ± 0.11 | Group F = 12.85 p = 0.0005 |
| | | | | | | | | Layer F = 39.21 p = 10−8 |
Deep | | | | | | | | | A × G F = 6.97 p = 10−6 |
Sham | 0.13 ± 0.07 | 0.29 ± 0.12 | 0.32 ± 0.08 | 0.45 ± 0.11 | 0.72 ± 0.28 | 0.94 ± 0.07 | 0.96 ± 0.04 | 0.87 ± 0.16 | A × L F = 3.24 p = 0.0035 |
Enucleated | 0.09 ± 0.05 | 0.25 ± 0.16 | 0.24 ± 0.10 | 0.52 ± 0.16 | 0.92 ± 0.11 | 0.65 ± 0.25 | 0.67 ± 0.22 | 0.87 ± 0.08 | G × L F = 1.71 p = 0.19 |
| | | | | | | | | A × G × L F = 0.44 p = 0.88 |
| | | | | | | | | |
Figure 4B | Distance vs P16–P17 distribution | | | | | | | | |
Superficial | P8–P9 | P10–P11 | | P12 | P13 | P14 | P15 | P16–P17 | |
Sham | 0.57 ± 0.05 | 0.49 ± 0.02 | | 0.51 ± 0.05 | 0.43 ± 0.03 | 0.45 ± 0.04 | 0.29 ± 0.02 | 0.27 ± 0.09 | Age F = 28.19 p < 10−10 |
Enucleated | 0.62 ± 0.08 | 0.52 ± 0.05 | | 0.42 ± 0.04 | 0.38 ± 0.03 | 0.37 ± 0.02 | 0.49 ± 0.06 | 0.38 ± 0.08 | Group F = 6.38 p = 0.0118 |
| | | | | | | | | Layer F = 14.29 p = 0.0002 |
Deep | | | | | | | | | A × G F = 2.20 p = 0.06 |
Sham | 0.79 ± 0.07 | 0.48 ± 0.03 | | 0.63 ± 0.08 | 0.55 ± 0.06 | 0.52 ± 0.05 | 0.42 ± 0.04 | 0.20 ± 0.09 | A × L F = 3.11 p = 0.0053 |
Enucleated | 0.82 ± 0.06 | 0.64 ± 0.05 | | 0.57 ± 0.06 | 0.55 ± 0.06 | 0.52 ± 0.03 | 0.53 ± 0.05 | 0.39 ± 0.08 | G × L F = 0.48 p = 0.49 |
| | | | | | | | | A × G × L F = 1.17 p = 0.32 |
| | | | | | | | | |
Figure 5B | Distance vs P16–P17 distribution | | | | | | | | |
Superficial | P8–P9 | P10–P11 | | P12 | P13 | P14 | P15 | P16–P17 | |
Sham | 0.68 ± 0.02 | 0.70 ± 0.03 | | 0.83 ± 0.07 | 0.49 ± 0.03 | 0.35 ± 0.05 | 0.29 ± 0.02 | 0.20 ± 0.07 | Age F = 69.16 p < 10−10 |
Enucleated | 0.72 ± 0.05 | 0.77 ± 0.07 | | 0.36 ± 0.03 | 0.41 ± 0.05 | 0.41 ± 0.03 | 0.48 ± 0.09 | 0.32 ± 0.09 | Group F = 11.06 p = 0.0009 |
| | | | | | | | | Layer F = 2.28 p = 0.13 |
Deep | | | | | | | | | A × G F = 13.28 p < 10−10 |
Sham | 0.78 ± 0.04 | 0.64 ± 0.04 | | 0.75 ± 0.07 | 0.51 ± 0.04 | 0.36 ± 0.04 | 0.29 ± 0.03 | 0.29 ± 0.07 | A × L F = 1.98 p = 0.067 |
Enucleated | 0.91 ± 0.05 | 0.88 ± 0.06 | | 0.48 ± 0.05 | 0.21 ± 0.03 | 0.42 ± 0.04 | 0.49 ± 0.07 | 0.33 ± 0.09 | G × L F = 0.07 p = 0.79 |
| | | | | | | | | A × G × L F = 2.60 p = 0.017 |