Table 1

Summary data and statistical analysis for Figures 1-13

Figures			Values			Statistical tests
Figure 1
			Control	Pyr:Kcnq2
1B	CA3	Duration	1.6 ± 0.46 s	2.2 ± 0.35 s	Mann–Whitney U test	p = 0.26	Control: n = 10; Pyr:Kcnq2: n = 18 hemispheres
	CA3	Events/s	0.0054 ± 0.0015	0.013 ± 0.0025	Mann–Whitney U test	p = 0.0143	Control: n = 22; Pyr:Kcnq2: n = 21 hemispheres
	Posterior cortex	Duration	1.15 ± 0.24 s	5.3 ± 0.61 s	Mann–Whitney U test	p < 0.0001	Control: n = 15; Pyr:Kcnq2: n = 20 hemispheres
	Posterior cortex	Events/s	0.008 ± 0.0018 Hz	0.015 ± 0.0018 Hz	Mann–Whitney U test	p = 0.0039	Control: n = 22; Pyr:Kcnq2: n = 20 hemispheres Animals: Control: n = 6; Pyr:Kcnq2: n = 6.
1C	CA3	APs vs current injections			Two-way ANOVA	(p = 0.0025; F_(9,33) = 3.745)	Control: n = 5; pPyr:Kcnq2: n = 6 cells
Figure 2
			Control	Pyr:Kcnq2	Statistical tests and values
2C	CA3	Amplitude	0.064 ± 0.007 △F/F	0.14 ± 0.014 △F/F	Mann–Whitney U test	p < 0.0001	Control: n = 46; Pyr:Kcnq2: n = 56 hemispheres
		Duration	1.4 ± 0.18 s	8.5 ± 0.68 s	Mann–Whitney U test	p < 0.0001	Control: n = 46; Pyr:Kcnq2: n = 56 hemispheres
		Events/s	0.26 ± 0.023 Hz	0.043 ± 0.0047 Hz	Mann–Whitney U test	p < 0.0001
	Posterior cortex (PC)	Amplitude	0.08 ± 0.006 △F/F	0.29 ± 0.023 △F/F	Mann–Whitney U test	p < 0.0001	Control: n = 44; Pyr:Kcnq2: n = 56 hemispheres
		Duration	2.2 ± 0.19 s	6.5 ± 0.37 s	Mann–Whitney U test	p < 0.0001	Control: n = 44; Pyr:Kcnq2: n = 56 hemispheres
		Events/s	0.14 ± 0.0072 Hz	0.048 ± 0.0032 Hz	Mann–Whitney U test	p < 0.0001
	Medial cortex (MC)	Amplitude	0.021 ± 0.01 △F/F	0.33 ± 0.029 △F/F	Mann–Whitney U test	p < 0.0001	Control, n = 43; Pyr:Kcnq2: n = 55 hemispheres
		Duration	1.4 ± 0.58 s	9.5 ± 0.56 s	Mann–Whitney U test	p < 0.0001	Control, n = 43; Pyr:Kcnq2: n = 55 hemispheres
		Events/s	0.0065 ± 0.0024 Hz	0.018 ± 0.0023 Hz	Mann–Whitney U test
	Anterior cortex (AC)	Amplitude	0.02 ± 0.003 △F/F	0.36 ± 0.03 △F/F	Mann–Whitney U test	p < 0.0001	Control: n = 44; Pyr:Kcnq2: n = 54 hemispheres
		Duration	1.5 ± 0.3 s	11.2 ± 0.8 s	Mann–Whitney U test	p < 0.0001	Control: n = 44; Pyr:Kcnq2: n = 54 hemispheres
		Events/s	0.012 ± 0.0021 Hz	0.021 ± 0.0025 Hz	Mann–Whitney U test	p = 0.0005
Data were obtained from 8 control and 9 Pyr:Kcnq2 mice.
Figure 3
			Control	Pyr:Kcnq2	Statistical tests and values
3C	Power 0.02–0.2 Hz		0.022 ± 0.0015 A.U.	0.11 ± 0.0046 A.U.	Mann–Whitney U test	p < 0.0001	Control, n = 43; Pyr:Kcnq2:, n = 47 hemispheres
3C	Power 0.2–2 Hz		0.026 ± 0.011 A.U.	0.38 ± 0.012A.U.	Mann–Whitney U test	p < 0.0001	Control, n = 43; Pyr:Kcnq2:, n = 47 hemispheres
3D	ROI 0.02–0.2 Hz		10.1 ± 0.7 ROIs	19.9 ± 0.7 ROIs	Mann–Whitney U test	p < 0.0001	Control, n = 43; Pyr:Kcnq2:, n = 47 hemispheres
3D	ROI 0.2–2 Hz		6.6 ± 0.5 ROIs	0.5 ± 0.2 ROIs	Mann–Whitney U test	p < 0.0001	Control, n = 43; Pyr:Kcnq2:, n = 47 hemispheres
Data were obtained from 8 control and 9 Pyr:Kcnq2 mice.
Figure 4
				Control	Pyr:Kcnq2	Statistical tests and values
4C	CA3	Amplitude	–PTX 0.057 △F/F ± 0.055 SD	–PTX 0.08 △F/F ± 0.09 SD	Mann–Whitney U test	Control p < 0.0001	Control: –PTX: n = 1547; +PTX: n = 1322 events Pyr:Kcnq2: –PTX: n = 224 events ; +PTX: n = 523 events
		Amplitude	+PTX 0.27 △F/F ± 0.12 SD	+PTX 0.19 △F/F ± 0.09 SD	Mann–Whitney U test	Pyr:Kcnq2 p < 0.0001
		Duration	–PTX 0.8 s ± 1.5 SD	–PTX 4.3 s ± 5.4 SD	Mann–Whitney U test	Control p < 0.0001
		Duration	+PTX 0.7 ± 1.7 SD	+PTX 1.8 ± 3.8 SD	Mann–Whitney U test	Pyr:Kcnq2 p < 0.0001
	Posterior cortex	Amplitude	–PTX 0.08 △F/F ± 0.058 SD	–PTX 0.21 △F/F ± 0.26 SD	Mann–Whitney U test	Control p < 0.0001	Control: –PTX: n = 931; +PTX: n = 777 events Pyr:Kcnq2: –PTX: n = 223; +PTX: n = 444 events
	Posterior cortex	Amplitude	+PTX 0.43 △F/F ± 0.20 SD	+PTX 0.38 △F/F ± 0.24 SD	Mann–Whitney U test	Pyr:Kcnq2 p < 0.0001
		Duration	–PTX 1.9 s ± 2.4 SD	–PTX 5.1 s ± 4.5 SD	Mann–Whitney U test	Control p < 0.0001
		Duration	+PTX 0.58 s ± 1.0 SD	+PTX 1.5 ± 1.9 SD	Mann–Whitney U test	Pyr:Kcnq2 p < 0.0001
	Medial cortex	Amplitude	–PTX 0.053 △F/F ± 0.066 SD	–PTX 0.21 △F/F ± 0.25 SD	Mann–Whitney U test	Control p < 0.0001	Control: –PTX: n = 94; +PTX: n = 270 events Pyr:Kcnq2: –PTX: n = 62; +PTX: n = 267 events
		Amplitude	+PTX 0.13 △F/F ± 0.22 SD	+PTX 0.27 △F/F ± 0.24 SD	Mann–Whitney U test	Pyr:Kcnq2 p = 0.0003
		Duration	–PTX 0.9 s ± 0.74 SD	–PTX 7.8 s ± 6.7 SD	Mann–Whitney U test	Control p < 0.0034
		Duration	+PTX 1.3 s ± 2.3 SD	+PTX 2.1 ± 3.3 SD	Mann–Whitney U test	Pyr:Kcnq2 p < 0.0001
	Anterior cortex	Amplitude	–PTX 0.048 △F/F ± 0.038 SD	–PTX 0.16 △F/F ± 0.26 SD	Mann–Whitney U test	Control: p < 0.0001	Control: –PTX: n = 125; +PTX: n = 310 events Pyr:Kcnq2: –PTX: n = 150; +PTX: n = 66 events
		Amplitude	+PTX 0.14 △F/F ± 0.1 SD	+PTX 0.37 △F/F ± 0.31 SD	Mann–Whitney U test	Pyr:Kcnq2 p < 0.0001
		Duration	–PTX 1.0 s ± 1.3 SD	–PTX 4.8 s ± 6.1 SD	Mann–Whitney U test	Control p = 0.11
		Duration	+PTX 0.8 s ± 0.9 SD	+PTX 5.3 ± 7.1 SD	Mann–Whitney U test	Pyr:Kcnq2 p = 0.87
Data were obtained from 3 control and 3 Pyr:Kcnq2 mice.
Figure 5
			Control	Pyr:Kcnq2	Statistical tests and values
5C	Power 0.02–0.2 Hz		–PTX 0.02 ± 0.003 A.U.	–PTX 0.11 ± 0.01 A.U.	Control Wilcoxon paired test	Control p < 0.0001	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.02–0.2 Hz		+PTX 0.06 ± 0.007 A.U.	+PTX 0.18 ± 0.01 A.U.	Pyr:Kcnq2 paired Student’s t test	p < 0.0001 t = 6.233, df = 13	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.2–2 Hz		–PTX 0.28 ± 0.02 A.U.	–PTX 0.37 ± 0.02 A.U.	Control Wilcoxon paired	Control p < 0.0001	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.2–2 Hz		+PTX 0.58 ± 0.03 A.U.	+PTX 0.73 ± 0.0 A.U.6	Pyr:Kcnq2 Wilcoxon paired test	Pyr:Kcnq2 p < 0.0001	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
5D	ROI 0.02–0.2 Hz		–PTX 8.8 ± 0.7 ROIs	–PTX 23.0 ± 1.4 ROIs	Control paired Student’s t test	Control p = 0.84 t = 0.2042, df = 19	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	ROI 0.02–0.2 Hz		+PTX 9.1 ± 1.1 ROIs	+PTX 25.2 ± 1.4 ROIs	Pyr:Kcnq2 paired Student’s t test	Pyr:Kcnq2 p = 0.19 t = 1.377, df = 13
	ROI 0.2–2 Hz		–PTX 7.6 ± 0.6 ROIs	–PTX 0 ROI	Control Wilcoxon paired test	Control p = 0.72	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	ROI 0.2–2 Hz		+PTX 8.7 ± 1.6 ROIs	+PTX 0 ROI	Pyr:Kcnq2 N/A	N/A
Data were obtained from 4 control and 3 Pyr:Kcnq2 mice.
Figure 6
			Control	Pyr:Kcnq2	Statistical tests and values
6C	CA3	Amplitude	–D-APV 0.070 △F/F ± 0.069 SD	–D-APV 0.13 △F/F ± 0.14 SD	Mann–Whitney U test	Control: p < 0.0001	Control: –D-APV n = 1265; +D-APV: n = 571 events Pyr:Kcnq2: –D-APV n = 193; +D-APV: n = 156 events
			+D-APV 0.036 △F/F ± 0.014 SD	+D-APV 0.09 △F/F ± 0.1 SD	Mann–Whitney U test	Pyr:Kcnq2: p < 0.0001
		Duration	–D-APV 0.88 s ± 2.0 SD	–D-APV 5.4 s ± 5.8 SD	Mann–Whitney U test	Control: p < 0.0001
			+D-APV 0.78 ± 1.2 SD	+D-APV 5.4 ± 7.1 SD	Mann–Whitney U test	Pyr:Kcnq2: p = 0.0055
	Posterior cortex	Amplitude	–D-APV 0.07 △F/F ± 0.049 SD	–D-APV 0.26 △F/F ± 0.36 SD	Mann–Whitney U test	Control: p < 0.0001	Control: –D-APV n = 543; +D-APV n = 201 events Pyr:Kcnq2: –D-APV n = 243; +D-APV n = 170 events
	Posterior cortex		+D-APV 0.02 △F/F ± 0.01 SD	+D-APV 0.13 △F/F ± 0.14 SD	Mann–Whitney U test	Pyr:Kcnq2: p < 0.0001
		Duration	–D-APV 1.99 s ± 2.1 SD	–D-APV 5.1 s ± 4.2 SD	Mann–Whitney U test	Control: p < 0.0001
		Duration		+D-APV 2.5s ± 2.7 SD	+D-APV 10.8 ± 6.6 SD	Mann–Whitney U test	Pyr:Kcnq2: p < 0.0001
	Medial cortex	Amplitude	–D-APV 0.11 △F/F ± 0.16 SD	–D-APV 0.18 △F/F ± 0.2 SD	Control: N/A	Control: N/A	Control –D-APV n = 10; +D-APV n = 0 events Pyr:Kcnq2 –D-APV n = 158; +D-APV, n = 50events;
			+D-APV N/A	+D-APV 0.14 △F/F ± 0.16 SD	Pyr:Kcnq2: Mann–Whitney U test	Pyr:Kcnq2: p = 0.22
		Duration	–D-APV 7.8s ± 8.4 SD	–D-APV 6.3 ± 6.2 SD	Control: N/A	Control: N/A
			+D-APV N/A	+D-APV 12.9 ± 8.4 SD	Pyr:Kcnq2: Mann–Whitney U test	Pyr:Kcnq2: p < 0.0001
	Anterior cortex	Amplitude	–D-APV 0.017 △F/F ± 0.006 SD	–D-APV 0.31 △F/F ± 0.4 SD	Control: N/A	Control: N/A	Control –D-APV n = 54; +D-APV n = 0 events Pyr:Kcnq2: –D-APV n = 71; +D-APV n = 29 events
			+D-APV N/A	+D-APV 0.15 △F/F ± 0.19 SD	Pyr:Kcnq2: Mann–Whitney U test	Pyr:Kcnq2: p = 0.0055
		Duration	–D-APV 2.5 s ± 3.2 SD	–D-APV 8.9 s ± 7.1 SD	Control: N/A	Control: N/A
			+D-APV N/A	+D-APV 13.3 ± 11 SD	Pyr:Kcnq2: Mann–Whitney U test	Pyr:Kcnq2: p = 0.04
Data were obtained from 3 control and 3 Pyr:Kcnq2 mice.
Figure 7
			Control	Pyr:Kcnq2	Statistical tests and values
7C	Power 0.02–0.2 Hz		–D-APV 0.019 ± 0.0015 A.U.	–D-APV 0.11 ± 0.0085 A.U.	Control: Wilcoxon paired	Control: p < 0.0001	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.02–0.2 Hz		+D-APV 0.014 ± 0.0015 A.U.	+D-APV 0.069 ± 0.0057 A.U.	Pyr:Kcnq2: paired Student’s t test	Pyr:Kcnq2: p < 0.0001, t = 12.64, df = 13	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.2–2 Hz		–D-APV 0.24 ± 0.012 A.U.	–D-APV 0.39 ± 0.022 A.U.	Control: Wilcoxon paired	Control: p = 0.0007	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.2–2 Hz		+D-APV 0.18 ± 0.017 A.U.	+D-APV 0.26 ± 0.0080 A.U.	Pyr:Kcnq2: Wilcoxon paired	Pyr:Kcnq2: p = 0.0001	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
7D	ROI 0.02–0.2 Hz		–D-APV 8.9 ± 0.65 ROIs	–D-APV 25.21 ± 1.3 ROIs	Control: paired Student’s t test	Control: p = 0.20 t = 1.32, df = 19	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	ROI 0.02–0.2 Hz		+D-APV 7.5 ± 0.92 ROIs	+D-APV 20.71 ± 1.0 ROIs	Pyr:Kcnq2: paired Student’s t test	Pyr:Kcnq2: p = 0.0002 t = 5.22, df = 13	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.2–2 Hz		–D-APV 7.05 ± 0.54 ROIs	–D-APV 0 ROIs	Control: paired Student’s t test	Control: p = 0.003 t = 3.472, df = 19	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.2–2 Hz		+D-APV 4.5 ± 0.54 ROIs	+D-APV 0.43 ± 0.2 ROIs	Pyr:Kcnq2: N/A	N/A	Control: n = 20; Pyr:Kcnq2: n = 14 hemispheres
Data were obtained from 3 control and 3 Pyr:Kcnq2 mice.
Figure 8
P/N/A refers to Picrotoxin/APV/NBQX			Control	Pyr:Kcnq2	Statistical tests and values
8B	CA3	Amplitude	–P/N/A: 0.046 Δ F/F ± 0.14 SD	–P/N/A:0.105 ΔF/F ± 0.14 SD	Mann–Whitney U test	Control: N/A	Control –P/N/A: n = 444; +P/N/A: n = 0 events Pyr:Kcnq2: –P/N/A: n = 246; +P/N/A: n = 38 events
			N/A	+P/N/A: ΔF/F 0.14 ± 0.13 SD		Pyr:Kcnq2 p = 0.0001
		Duration	–P/N/A: 0.59 s ± 1.3 SD	–P/N/A: 4.5 s ± 6.7 SD	Mann–Whitney U test	Control: N/A
			N/A	+P/N/A: 13.5 s ± 7.5 SD		Pyr:Kcnq2: p < 0.0001
	Posterior cortex	Amplitude	0.059 Δ F/F ± 0.03 SD	–P/N/A:0.48 Δ F/F ± 0.63 SD	Mann–Whitney U test	Control: N/A	Control –P/N/A: n = 228; +P/N/A: n = 0 events Pyr:Kcnq2: –P/N/A: n = 194; +P/N/A: n = 36 events
	Posterior cortex		N/A	+P/N/A: Δ 0.71 F/F ± 0.47 SD		Pyr:Kcnq2: p = 0.009
		Duration	–P/N/A: 1.66 s ± 1.7 SD	–P/N/A: 4.6 s ± 3.9 SD	Mann–Whitney U test	Control: N/A
		Duration		N/A	+P/N/A: 10.2 s ± 3.6 SD		Pyr:Kcnq2: p < 0.0001
	Medial cortex	Amplitude	0.028 Δ F/F ± 0.018 SD	–P/N/A:0.38 Δ F/F ± 0.31 SD	Mann–Whitney U test	Control: N/A	Control –P/N/A: n = 22; +P/N/A: n = 0 events Pyr:Kcnq2: –P/N/A: n = 64; +P/N/A: n = 56 events
			N/A	+P/N/A: Δ 0.19 F/F ± 0.17 SD		Pyr:Kcnq2: p = 0.005
		Duration	–P/N/A: 0.65 s ± 0.17 SD	–P/N/A: 7.6 s ± 4.7 SD	Mann–Whitney U test	Control: N/A
			N/A	+P/N/A: 8.9 s ± 7.5 SD		Pyr:Kcnq2: p = 0.087
	Anterior cortex	Amplitude	0.029 Δ F/F ± 0.026 SD	–P/N/A:0.47 Δ F/F ± 0.48 SD	Mann–Whitney U test	Control: N/A	Control –P/N/A: n = 22; +P/N/A: n = 0 events Pyr:Kcnq2: –P/N/A: n = 82; +P/N/A: n = 43 events
			N/A	+P/N/A: Δ 0.35 F/F ± 0.38 SD		Pyr:Kcnq2: p = 0.087
		Duration	–P/N/A: 0.77 s ± 0.63 SD	–P/N/A: 7.7 s ± 5.8 SD	Mann–Whitney U test	Control: N/A
			N/A	+P/N/A: 14.1 s ± 8.7 SD		Pyr:Kcnq2: p = 0.016
Data were obtained from 3 control and 3 Pyr:Kcnq2 mice.
Figure 9
P/N/A refers to Picrotoxin/APV/NBQX			Control	Pyr:Kcnq2	Statistical tests and values
9C,D	Power 0.02–0.2 Hz		–P/A/N 0.018 ± 0.0007 A.U.	–P/A/N 0.13 ± 0.0094 A.U.	Control: Wilcoxon paired	Control: p < 0.0001	Control: n = 18; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.02–0.2 Hz		+P/A/N 0.01 ± 0.0006 A.U.	+P/A/N 0.068 ± 0.0083 A.U.	Pyr:Kcnq2: Wilcoxon paired	Pyr:Kcnq2: p = 0.0001	Control: n = 18; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.2–2 Hz		–P/A/N 0.22 ± 0.007 A.U.	–P/A/N 0.45 ± 0.03 A.U.	Control: paired Student’s t test	Control: p < 0.0001 t = 9.106, df = 17	Control: n = 18; Pyr:Kcnq2: n = 14 hemispheres
	Power 0.2–2 Hz		+P/A/N 0.13 ± 0.002 A.U.	+P/A/N 0.26 ± 0.01 A.U.	Pyr:Kcnq2: paired Student’s t test	Pyr:Kcnq2: p < 0.0001 t = 9.055, df = 13	Control: n = 18; Pyr:Kcnq2: n = 14 hemispheres
	ROI 0.02–0.2 Hz		–P/A/N 10.17 ± 0.93 ROIs	–P/A/N 19.86 ± 1.2 ROIs	Control: Wilcoxon paired	Control: p = 0.0001	Control: n = 18; Pyr:Kcnq2: n = 14 hemispheres
	ROI 0.02–0.2 Hz		+P/A/N 3.5 ± 0.85 ROIs	+P/A/N 15.14 ± 1.3 ROIs	Pyr:Kcnq2: paired Student’s t test	Pyr:Kcnq2: p = 0.0092 t = 3.057, df = 13	Control: n = 18; Pyr:Kcnq2: n = 14 hemispheres
	ROI 0.2–2 Hz		–P/A/N 8.7 ± 0.7 ROIs	–P/A/N 0 ROI	Control: Wilcoxon paired	Control: p < 0.0001	Control: n = 18; Pyr:Kcnq2: n = 14 hemispheres
	ROI 0.2–2 Hz		+P/A/N 0.72 ± 0.18 ROIs	+P/A/N 0 ROI	Pyr:Kcnq2: N/A		Control: n = 18; Pyr:Kcnq2: n = 14 hemispheres
Data were obtained from 3 control and 3 Pyr:Kcnq2 mice.
Figure 10
			Kcnq3^+/+	Kcnq3^−/−	Statistical tests
10C	CA3	Amplitude	0.08 ± 0.005△F/F	0.08 ± 0.007△F/F	Welch’s t test	p = 0.95, t = 0.07052, df = 42	Kcnq3^+/+, n = 23; Kcnq3^−/−: n = 21 hemispheres
		Duration	2.4 ± 0.8 s	1.2 ± 0.2 s	Mann–Whitney U test	p = 0.93	Kcnq3^+/+, n = 23; Kcnq3^−/−: n = 21 hemispheres
		Events/s	0.22 ± 0.03 Hz	0.19 ± 0.3 Hz	Mann–Whitney U test	p = 0.05	Kcnq3^+/+, n = 24; Kcnq3^−/−: n = 24 hemispheres
	Posterior cortex	Amplitude	0.073 ± 0.006△F/F	0.12 ± 0.01△F/F	Mann–Whitney U test	p < 0.0001	Kcnq3^+/+, n = 23; Kcnq3^−/−^, n = 24 hemispheres
		Duration	1.4 ± 0.3 s	4.5 ± 0.9 s	Mann–Whitney U test	p < 0.0001	Kcnq3^+/+, n = 23; Kcnq3^−/−^, n = 24 hemispheres
		Events/s	0.21 ± 0.025 Hz	0.12 ± 0.023 Hz	Mann–Whitney U test	p = 0.013	Kcnq3^+/+, n = 24; Kcnq3^−/−: n = 24 hemispheres
	Medial cortex	Amplitude	0.028 ± 0.008△F/F	0.09 ± 0.02△F/F	Mann–Whitney U test	p = 0.08	Kcnq3^+/+, n = 13; Kcnq3^−/−: n = 21 hemispheres
		Duration	3.2 ± 1.1 s	4.3 ± 1.2 s	Mann–Whitney U test	p = 0.004	Kcnq3^+/+, n = 13; Kcnq3^−/−: n = 21 hemispheres
		Events/s	0.02 ± 0.12 Hz	0.017 ± 0.003 Hz	Mann–Whitney U test	p = 0.009	Kcnq3^+/+, n = 24; Kcnq3^−/−: n = 24 hemispheres
	Anterior cortex	Amplitude	0.03 ± 0.003△F/F	0.10 ± 0.13△F/F	Mann–Whitney U test	p < 0.0001	Kcnq3^+/+, n = 20; Kcnq3^−/−: n = 22 hemispheres
		Duration	3.1 ± 1.0 s	5.2 ± 1.3 s	Mann–Whitney U test	p = 0.72	Kcnq3^+/+, n = 20; Kcnq3^−/−: n = 22 hemispheres
		Events/s	0.032 ± 0.007 Hz	0.05 ± 0.007 Hz	Mann–Whitney U test	p = 0.06	Kcnq3^+/+, n = 24; Kcnq3^−/−: n = 24 hemispheres
Data were obtained from 4 Kcnq3^+/+ and 4 Kcnq3^−/− mice.
Figure 11
			Kcnq3^+/+	Kcnq3^−/−	Statistical tests
11D	Power 0.02–0.2 Hz		0.035 ± 0.005 A.U.	0.06 ± 0.005 A.U.	Mann–Whitney U test	p < 0.0001	Kcnq3^+/+, n = 24; Kcnq3^−/−: n = 24 hemispheres
	Power 0.2–2 Hz		0.57 ± 0.1 A.U.	0.53 ± 0.08 A.U.	Mann–Whitney U test	p = 0.67	Kcnq3^+/+, n = 24; Kcnq3^−/−: n = 24 hemispheres
	ROI 0.02–0.2 Hz		12 ± 1 ROIs	25 ± 1 ROIs	Mann–Whitney U test	p < 0.0001	Kcnq3^+/+, n = 24; Kcnq3^−/−: n = 24 hemispheres
	ROI 0.2–2 Hz		6.2 ± 0.8 ROIs	0.6 ± 0.2 ROIs	Mann–Whitney U test	p < 0.0001	Kcnq3^+/+, n = 24; Kcnq3^−/−: n = 24 hemispheres
Data were obtained from 4 Kcnq3^+/+ and 4 Kcnq3^−/− mice.
Figure 12
P/N/A refers to Picrotoxin/APV/NBQX			Kcnq3^−/− –blockers	Kcnq3^−/− +blockers	Statistical tests
12B	CA3	Amplitude	0.09 ± 0.009 △F/F	0.064 ± 0.008 △F/F	Student’s t test	p = 0.054, t = 2.007, df = 29	Kcnq3^−/−: n = 16; +P/A/N: n = 15 hemispheres
		Duration (not illustrated)	1.1 ± 0.2 s	7.7 ± 1.6 s	Wilcoxon paired	p = 0.0006	Kcnq3^-/: n = 16; P/A/N: n = 15 hemispheres
		Events/s	0.22 ± 0.03 Hz	0.019 ± 0.3 Hz	Wilcoxon paired test	p = 0.0002	Kcnq3^−/− n = 18; P/A/N: n = 18 hemispheres
	Posterior cortex	Amplitude	0.13 ± 0.01 △F/F	0.17 ± 0.02 △F/F	Wilcoxon paired	p = 0.15	Kcnq3^−/− n = 18; P/A/N: n = 15 hemispheres
		Duration (not illustrated)	3.1 ± 0.8 s	9.0 ± 0.9 s	Wilcoxon paired	p = 0.0006	Kcnq3^−/− n = 18; P/A/N: n = 15 hemispheres
		Events/s	0.16 ± 0.03 Hz	0.01 ± 0.003 Hz	Wilcoxon paired	p < 0.0001	Kcnq3^−/− n = 18; P/A/N: n = 18 hemispheres
	Medial cortex	Amplitude	0.12 ± 0.02△F/F	ND	N/D	N/D	Kcnq3^−/− n = 18; +P/A/N: n = 0 hemispheres
		Duration (not illustrated)	5.2 ± 1.6 s	ND	N/D	N/D	Kcnq3^−/− n = 18; P/A/N: n = 0 hemispheres
		Events/s	0.02 ± 0.004 Hz	ND	N/D	N/D	Kcnq3^−/− n = 18; P/A/N: n = 18 hemispheres
	Anterior cortex	Amplitude	0.1 ± 0.1△F/F	0.07 ± 0.02△F/F	Wilcoxon paired	p = 0.88	Kcnq3^−/− n = 18; P/A/N: n = 4 hemispheres
		Duration (not illustrated)	4.0 ± 0.7 s	9.6 ± 3.4 s	Wilcoxon paired	p = 0.25	Kcnq3^−/− n = 18; P/A/N: n = 4 hemispheres
		Events/s	0.06 ± 0.007 Hz	0.001 ± 0.0006 Hz	Wilcoxon paired	p < 0.0001	Kcnq3^−/− n = 18; P/A/N: n = 18 hemispheres
Data were obtained from 3 Kcnq3^+/+ and 3 Kcnq3^−/− mice.
Figure 13
P/N/A refers to Picrotoxin/APV/NBQX			Kcnq3^+/+	Kcnq3^−/−	Statistical tests
13C	Power 0.02–0.2 Hz		–P/A/N 0.025 ± 0.0019	–P/A/N 0.05 ± 0.003	Kcnq3^+/+ Wilcoxon paired	p < 0.0001	Kcnq3^+/+ n = 17; Kcnq3^−/− n = 16 hemispheres
	Power 0.02–0.2 Hz		+P/A/N 0.01 ± 0.0009	+P/A/N 0.02 ± 0.0018	Kcnq3^−/− paired Student’s t test	p < 0.0001 t = 9.531, df = 15
	Power 0.2–2 Hz		–P/A/N 0.33 ± 0.012	–P/A/N 0.33 ± 0.019	Kcnq3^+/+ paired Student’s t test	p < 0.0001 t = 11.60, df = 16
	Power 0.2–2 Hz		+P/A/N 0.19 ± 0.006	+P/A/N 0.19 ± 0.011	Kcnq3^−/− paired Student’s t test	p < 0.0001 t = 8.787, df = 15
13D	ROI 0.02–0.2 Hz		–P/A/N 12.5 ± 0.96	–P/A/N 25 ± 1.3	Kcnq3^+/+ Wilcoxon paired	p = 0.0004 t = 4.477, df = 16	Kcnq3^+/+ n = 17; Kcnq3^−/− n = 16 hemispheres
	ROI 0.02–0.2 Hz		+P/A/N 6.0 ± 1.1	+P/A/N 17.7 ± 1.6	Kcnq3^−/− paired Student’s t test	p = 0.0006 t = 4.321, df = 15	Kcnq3^+/+ n = 17; Kcnq3^−/− n = 16 hemispheres
	ROI 0.2–2 Hz		–P/A/N 7.1 ± 0.92	–P/A/N 0.75 ± 0.25	Kcnq3^+/+ Wilcoxon paired	p < 0.0001
	ROI 0.2–2 Hz		+P/A/N 0.76 ± 0.22	+P/A/N 0.81 ± 0.26	Kcnq3^−/−Wilcoxon paired	p = 0.83
Data were obtained from 3 Kcnq3^+/+ and 3 Kcnq3^−/− mice