Table 1

Antisense riboprobes used for RNA ISH

ProbePrimers (5′−3′)Linearize/transcribe
bhlhe40F-TTGCAAATCGGCGAACAGGG
R-GGAAACGTGCACGCAGTCG
EcoRV/SP6
foxd1F-AGGCAACTACTGGACGCTAGACCCTG
R-GAAACAGACCGTGTAAAAATATCACACTCCGAG
EcoRV/SP6
foxg1aF-GCAGGAAGAAAAACGGGACGC
R-GATGGGTGAGGGACATGGGG
EcoRV/SP6
plxna1aF-ACGGGTCAGTTATCGCCCTG
R-CGCCGACAGGATCTCGTCTT
EcoRV/SP6
plxna1bF-CTCAGCCGGAAAACACATGG
R-GAACTTCACCTCCGGGTTTCTG
EcoRV/SP6
sema6dF-GCAGTAGCAGTCAACGTTCTG
R-GGGGTCAGTAGTTGTGTGTCGT
BamH1/T7
tbx5F-GGAGCTGCATCGCATGTCAC
R-TGTCCAGTGCTCCTTTACCCC
EcoRV/SP6
tfecF-TATAAAGACCGGACGGGGACAAC
R-GCTCCTGGATTCGTAGCTGGA
EcoRV/SP6
vax2F-TGACAGGAACGAACTTCGCTAGAC
R-TCCGCTTCATCCGATTGGATGTTTGC
PmeI/T7