Quantitative analysis of Kv2.1 and Kv2.2 immunoreactivities in cartwheel cells
| Cell bodies in p27 male | Cell bodies in p30 male | Cell bodies in p34 female | Cell bodies in the three mice | AISs in the three mice | Proximal dendrites in biocytin-filled cells | |
|---|---|---|---|---|---|---|
| Kv2.1(+) and Kv2.2 (+)/total | 25/37 (67.6%) | 31/43 (72.1%) | 36/50 (72.0%) | 92/130 (70.8%) | 0/12 (0.0%) | 0/10 (0.0%) |
| Kv2.1(−) and Kv2.2 (+)/total | 11/37 (29.7%) | 10/43 (23.3%) | 13/50 (26.0%) | 34/130 (26.2%) | 0/12 (0.0%) | 0/10 (0.0%) |
| Kv2.1(+) and Kv2.2 (-)/total | 0/37 (0%) | 0/43 (0%) | 0/50 (0%) | 0/130 (0%) | 0/12 (0.0%) | 0/10 (0.0%) |
| Kv2.1(−) and Kv2.2 (−)/total | 1/37 (2.7%) | 2/43 (4.7%) | 1/50 (2.0%) | 4/130 (3.1%) | 12/12 (100.0%) | 10/10 (100.0%) |
For the examination of immunoreactivity to cell bodies and AISs, ryanodine-positive, medium-sized cells were considered as cartwheel cells. When cartwheel cells were labeled intracellularly, the cell type was confirmed by recording the characteristic complex spiking behavior.