Table 1

Odorants and concentrations used

Odor name	Odor number	Final estimated ppm (by experiment type)
Odor name	Odor number	ITA	1 Color	2 Color	Awake	Conc. 1x
Butyric acid	1		0.08	0.08
2-Methylbutyric acid	2		0.03	0.03	0.03
Valeric acid	3		0.03	0.03
Hexanoic acid	5	0.2	0.01	0.01	0.01	0.05
Isovaleric acid	6		0.04	0.04
2-Methylbuteraldehyde	9		2	2
Trans-2-methyl-2-butenal	10		0.7	0.7	0.7
Heptanal	11		0.7	0.7
2-Methylvaleraldehyde	13		3	3	3
Ethyl butyrate	19	10				2
Vinyl butyrate	20	9	2	2	2	3
Methyl valerate	21	8			5	2
Ethyl tiglate	22	2			0.6	0.6
Hexyl tiglate	23		1	1	1
s-Methyl thiobutanoate	24		5	5
Hexyl acetate	25		3	0.3
Isopropyl tiglate	27		5	5	5
Isoamylacetate	28		1	1
2-Hexanone	29	10			12	3
Menthone	32	0.01
2-Butanone	33		16	16		19
1-Hexanol	40		2	2
Cyclohexylamine	43		15	15	15
n-Methyl piperidine	45		1	1
2-methoxy-3-methylpyrazine	51			1	1
2-Isopropyl-3-methoxypyrazine	56		0.2	0.2
Benzaldehyde	57	0.1
Methyl benzoate	60	0.3				0.08
Isobutylthiazole	81			0.07	0.07
3-Octen-2-one	102		4	4
Trans-2-dodecanal	107		0.2
Furfuyl mercaptan	110		0.7
Isobutyraldehyde	136		5	5		30

Odorants and concentrations used in each experimental dataset. Odor number represents the internal reference number associated with each odorant, used in Figure 3. ITA; Figure 2, 1 color: iGluSnFR or SF-iGluSnFR imaged in anesthetized mice; Figure 3, 2 color: SF-iGluSnFR.A184S and jRGECO1a imaged simultaneously; Figures 6, 7. Awake: data from awake, head-fixed mice; Figures 5, 7, Conc (1×): lowest concentration of concentration series experiments; Figure 4, concentrations given as final estimated ppm, or the estimated vapor concentration of each odorant delivered to the animal, estimated from liquid dilution ratios, reported vapor pressures, and calibration of the odor delivery device.