Table 2

List of oligonucleotides used in this study

Primer code                               Sequence  CommentGene
PRS 40CGT GGC GAG ACC CTC GACForward externalexp-1
PRS 41CTC GAA CGT AGC CGC CAA TTCForward internalexp-1
PRS 42GTG CCA ACT TTA TCA GGG AGA GReverse externalexp-1
PRS 439AGGACGGAAATTACCTGTGCForward externaldaf-7
PRS 440GCTTCGGGAAACGCTCATATReverse externaldaf-7
PRS 441TTATTCTTTCTTGTCGGGGCCReverse internaldaf-7
PRS 362CTATTGCTTGCGCTCATAGGAAGGenotyping forward externalstr-2
PRS 363CTTCAGTCATCATGTTGCACAATTTCGenotyping reverse externalstr-2
PRS 364GTGTAATGTGGATTATACTTGTCAGGenotyping reverse externalstr-2
PRS 426AGCTGTTGTCTCCTTCCAGGGenotyping forward WTtax-2
PRS 427CGATTTCCGATGAGGAAACCGenotyping forward mutanttax-2
PRS 428CACAGCTTCTAATAGGAAAGGGGenotyping reverse Commontax-2
PRS 394GCGGTTCGGATACGAAAATACTTGGenotyping forward externaltax-4
PRS 395GACGGAGAAGTGTATCCGTTATATCGenotyping reverse internaltax-4
PRS 396CCATGCGTCCGTCCCTAATCCGenotyping reverse externaltax-4
PRS 403GTGTGGTACCCAAGTTGATAGGGenotyping forward commonceh-36
PRS 404GTTTTCCGCGAAACACAGTACCGenotyping reverse WTceh-36
PRS 405GTTTTCCGCGAAACACAGTACTGenotyping reverse Mceh-36
PRS 576GGAGAGTCGTCATCGGCGGenotyping forward externaldbl-1
PRS 577GGCATTGGATTTGGACAAGAGCGenotyping reverse externaldbl-1
PRS 578CTGTGCAGACTGGTCCGAGGenotyping forward internaldbl-1
PRS 349AAAACTGCAGcttgagagatccaatgaaatcggCloning forward PstIexp-1 promoter in pPD95.75
PRS 351CTAGTCTAGAgccatcaagttttggcagCloning reverse XbaIexp-1 promoter in pPD95.75
PRS 352GGGGTACCatgtctgcatctattctaattttgCloning forward KpnIexp-I Genomic in pPD95.75
PRS 353CCGCTCGAGctagtagatgtcggcaaaccactcCloning reverse XhoIexp-I Genomic in pPD95.75
PRS 369ACATGCATGCgaacggtctgtgggctctgacCloning forward SphIstr-2 promoter
PRS 370AAAACTGCAGgcagacccatatgtgtgcacaaacCloning reverse PstIstr-2 promoter
PRS 515ACATGCATGCgaagccttgtttgtataagaaacgctgCloning forward SphIgpa-4 promoter
PRS516CCCCCCGGGGttgaaaagtgttcacaaaatgaataagtgCloning reverse XmaIgpa-4 promoter
PRS 561CACTTTTCAACGGatccccgggatgccgactgtgcaatggCloning forward XmaIstr-2 genomic in a wrmScarlet vector
PRS 566GGGGTACCgcttgcgctcataggaagagCloning reverse KpnIstr-2 genomic in a wrmScarlet vector
PRS 534ACATGCATGCcggtcaccattcccaaattcgcCloning forward SphIdaf-1 promoter
PRS 535GGGGTACCcttgcacaggtaccaatttatgatgCloning reverse KpnIdaf-1 promoter