N/A, not applicable. Results of Cas-OFFinder analysis of sgRNA72 targets on mouse chromosome 2. The intended target for sgRNA72 is within Scn1a, shown in green. The location of each of the three potential off-target sites on mouse chromosome 2 identified by Cas-OFFinder is shown. The mismatches are indicated by lower case bold letters. The underlined region indicates the 10-nucleotide seed region proximal to the PAM site. The vertical line at the 3′ end of each target or off-target site indicates the boundary between the gRNA target and the adjacent Cas9 PAM (NGG) sequence. No off-target effects were found when Scn1a K1270T WT and heterozygous mouse DNA was amplified using specific primers (shown in Extended Data Table 1-1) and sequences were analyzed (Extended Data Fig. 1-1).