Table 2.

Summary of statistical tests

				Post hoc comparisons
Figure	Test	Results	p value		95.00% CI of diff.	Mean 1	Mean 2	Sig.
1B	Two-way ANOVA	Interaction: F_(50,816) = 0.1519	>0.9999
		Frequency: F_(50,816) = 31.82	<0.0001
		Genotype: F_(1,816) = 2.519	0.1129
1D	Two-way ANOVA	Interaction: F_(50,765) = 1.637	0.0043	1 Hz	−85.94 to −8.531	134.17	181.41	**
		Frequency: F_(50,765) = 47.27	<0.0001	2 Hz	−99.88 to −22.47	146.01	207.19	****
		Genotype: F_(1,765) = 18.97	<0.0001	3 Hz	−95.39 to −17.98	113.21	169.89	****
				4 Hz	−85.79 to −8.374	87.19	134.27	**
1F	Two-way ANOVA	Interaction: F_(50,561) = 2.252	<0.0001	1 Hz	−227.3 to −66.59	214.79	361.74	****
		Frequency: F_(50,561) = 30.41	<0.0001	2 Hz	−245.6 to −84.88	220.65	385.89	****
		Genotype: F_(1,561) = 29.53	<0.0001	3 Hz	−199.9 to −39.24	157.28	276.87	****
				4 Hz	−162.9 to −2.170	109.55	192.08	*
1H	Two-way ANOVA	Interaction: F_(50,408) = 1.149	0.2358	1 Hz	−129.1 to −21.47	191.40	266.67	***
		Frequency: F_(50,408) = 41.52	<0.0001	2 Hz	−128.2 to −20.64	184.40	258.83	***
		Genotype: F_(1,408) = 13.53	0.0003
1-1B	Unpaired t test with Welch’s correction	t = 10.40, df = 4.063; 95% CI: −1.208 to −0.7016	0.0004
1-2B	Two-way ANOVA	Interaction: F_(50,867) = 0.9298	0.6134	1 Hz	−114.5 to −9.244	142.99	204.87	**
		Frequency: F_(50,867) = 29.69	<0.0001	2 Hz	−124.7 to −19.43	160.32	232.38	***
		Genotype: F_(1,867) = 5.405	0.0203
1-2D	Two-way ANOVA	Interaction: F_(50,408) = 0.7116	0.9303	1 Hz	−134.8 to −34.49	114.52	199.17	****
		Frequency: F_(50,408) = 18.65	<0.0001
		Genotype: F_(1,408) = 0.7579	0.3845
2B	Two-way ANOVA	Interaction: F_(50,765) = 0.2408	>0.9999
		Frequency: F_(50,765) = 20.50	<0.0001
		Genotype: F_(1,765) = 0.4037	0.5254
2D	Two-way ANOVA	Interaction: F_(50,765) = 1.665	0.0033	1 Hz	−1193 to −269.8	1747.29	2478.75	****
		Frequency: F_(50,765) = 38.40	<0.0001	2 Hz	−1232 to −308.9	1407.57	2178.16	****
		Genotype: F_(1,765) = 15.16	0.0001	3 Hz	−1050 to −126.7	876.49	1464.83	**
2F	Two-way ANOVA	Interaction: F_(50,561) = 2.855	<0.0001	1 Hz	−2614 to −986.7	2801.60	4602.08	****
		Frequency: F_(50,561) = 52.60	<0.0001	2 Hz	−2674 to −1047	3021.36	4881.72	****
		Genotype: F_(1,561) = 21.59	<0.0001	3 Hz	−2135 to −507.5	2254.47	3575.70	****
				4 Hz	−1687 to −59.74	1626.22	2499.73	*
2H	Two-way ANOVA	Interaction: F_(50,255) = 0.8056	0.8194	1 Hz	−2861 to −279.7	2253.00	3823.60	**
		Frequency: F_(50,255) = 13.37	<0.0001	2 Hz	−2768 to −186.7	2214.00	3691.60	**
		Genotype: F_(1,255) = 10.13	0.0016

2-1B	Two-way ANOVA	Interaction: F_(50,867) = 2.797	<0.0001		−1659 to −404.5	2472.56	3504.52	****
		Frequency: F_(50,867) = 49.22	<0.0001		−2014 to −759.1	2199.18	3585.74	****
		Genotype: F_(1,867) = 26.10	<0.0001	3 Hz	−1828 to −572.9	1373.97	2574.32	****
				4 Hz	−1512 to −257.5	965.80	1850.69	***
				5 Hz	−1265 to −9.782	825.34	1462.54	*
2-1D	Two-way ANOVA	Interaction: F_(50,306) = 1.048	0.3937	1 Hz	−733.2 to −164.3	1002.67	1451.40	****
		Frequency: F_(50,306) = 46.53	<0.0001	2 Hz	−605.8 to −36.91	1032.67	1354.00	*
		Genotype: F_(1,306) = 9.863	0.0019
3B, dark cycle	Unpaired t test with Welch’s correction	t = 2.612, df = 10.98; 95% CI: 0.1150–1.347	0.0242
3B, light cycle		t = 2.480, df = 11.70; 95% CI: 0.08133–1.287	0.0294
3D, dark cycle	Unpaired t test with Welch’s correction	t = 2.296, df = 8.633; 95% CI: 0.01022–2.409	0.0485
3D, light cycle	Unpaired t test with Welch’s correction	t = 2.116, df = 8.641; 95% CI: −0.1092–2.980	0.0647
4A, latency clonus	Unpaired t test	t = 0.08745, df = 21; 95% CI: −138.9–127.7	0.9311
4A, latency seizure	Unpaired t test with Welch’s correction	t = 0.4379, df = 9.041; 95% CI: −481.5–325.2	0.6717
4A, recovery	Unpaired t test	t = 0.6691, df = 21; 95% CI: −1127–2197	0.5107
4B, latency clonus	Unpaired t test	t = 0.4538, df = 28. 95% CI: −47.05–29.98	0.6534
4B, latency seizure	Unpaired t test	t = 0.5004, df = 25; 95% CI: −13.37–21.95	0.6211
4B, recovery	Unpaired t test	t = 1.693, df = 30; 95% CI: −18.96–202.6	0.1009
4C, latency clonus	Unpaired t test	t = 1.605, df = 25; 95% CI: −18.48–2.295	0.1211
4C, latency seizure	Unpaired t test	t = 0.7429, df = 23; 95% CI: −13.22–6.234	0.4651
4C, recovery	Unpaired t test	t = 2.523, df = 19; 95% CI: 237.9–2552	0.0207
4-1A, immobility	Unpaired t test	t = 0.1999, df = 14; 95% CI: −39.49–32.76	0.8444
4-1A, recovery	Unpaired t test	t = 1.531, df = 14; 95% CI: −47.37–283.8	0.148
4-1B, immobility	Unpaired t test	t = 2.074, df = 22; 95% CI: −69.50 to −0.002622	0.05
4-1B, recovery	Unpaired t test	t = 3.054, df = 22; 95% CI: 113.7–595.3	0.0058
4-1C, immobility	Unpaired t test	t = 0.5781, df = 26; 95% CI: −22.82–40.68	0.5682
4-1C, recovery	Unpaired t test	t = 0.2767, df = 26; 95% CI: −57.80–44.09	0.7842
5C, 30 min: cortical	Unpaired t test	t = 0.1210, df = 5; 95% CI: t = 0.1210, df = 5	0.9084
5C, 30 min: hippocampal	Unpaired t test	t = 0.07701, df = 5; 95% CI: −22.03–20.75	0.9416
5C, 3 h: cortical	Unpaired t test	t = 3.039, df = 5; 95% CI: 4.279–51.22	0.0288
5C, 3 h hippocampal	Unpaired t test	t = 3.098, df = 5; 4.972–53.41	0.0269
6C	Unpaired t test	t = 2.806, df = 13; 95% CI: −5.558 to −0.7225	0.0149

*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.