Analysis | Mean ± SEM | Treatment |
---|---|---|
IBA1 (two-way ANOVA, Sidak’s multiple comparisons test) | 4 weeks: Control 0.06 ± 0.004 (n = 6), BLZ945 0.05 ± 0.01 (n = 6); 7 weeks: Control 0.04 ± 0.01 (n = 4), BLZ945 0.04 ± 0.003 (n = 7) | 4-week Control-BLZ945: p = 0.0574, t = 2.36, df = 19; 7-week Control-BLZ945: p = 0.9113, t = 0.388, df = 19; Control 4–7 weeks: p = 0.0166, t = 2.943, df = 19; BLZ945 4–7 weeks: p = 0.8425, t = 0.529, df = 19 |
GFAP (two-way ANOVA, Sidak’s multiple comparisons test) | 4 weeks: Control 0.05 ± 0.005 (n = 11, BLZ945 0.04 ± 0.005 (n = 6); 7 weeks: Control 0.04 ± 0.003 (n = 6), BLZ945 0.04 ± 0.002 (n = 8) | 4-week Control-BLZ945: p = 0.0258, t = 2.66, df = 27; 7-week Control-BLZ945: p = 0.8450, t = 0.522, df = 27; Control 4–7 weeks: p = 0.2535, t = 1.537, df = 27; BLZ945 4–7 weeks: p = 0.8381, t = 0.534, df = 27 |
Calyx of Held surface area (two-way ANOVA, Sidak’s multiple comparisons test) | 3 weeks: Control 687.5 ± 35.70 (n = 4), BLZ945 680.1.8 ± 13.40 (n = 4); 4 weeks: Control 747.1 ± 21.19 (n = 5), BLZ945 707.8 ± 56.78 (n = 5) | 3-week Control-BLZ945: p = 0.9895, t = 0.1310, df = 14; 4-week Control-BLZ945: p = 0.7005, t = 0.7724, df = 14; Control 3–4 weeks: p = 0.4941, t = 1.103, df = 14; BLZ945 3–4 weeks: p = 0.8527, t = 0.5126, df = 14 |
Polyinnervatioon of MNTB neurons (two-way ANOVA, Sidak’s multiple comparisons test) | 3 weeks: Control 9.92 ± 6.75 (n = 4), BLZ945 38.78 ± 9.89 (n = 4); 4 weeks: Control 5.08 ± 3.15 (n = 5), BLZ945 16.59 ± 5.82 (n = 5) | 3-week Control-BLZ945: p = 0.0191, t = 2.998, df = 14; 4-week Control-BLZ945: p = 0.3645, t = 1.336, df = 14; Control 3–4 weeks: p = 0.8435, t = 0.53, df = 14; BLZ945 3–4 weeks: p = 0.0574, t = 2.43, df = 14 |
Calyceal shape factor analysis (two-way ANOVA, Sidak’s multiple comparisons test) | 3 weeks: Control 6.394 ± 0.16 (n = 4), BLZ945 5.6 ± 0.17 (n = 4); 4 weeks: Control 6.74 ± 0.13 (n = 5), BLZ945 6.35 ± 0.24 (n = 5) | 3-week Control-BLZ945: p = 0.3210, t = 1.425, df = 14; 4-week Control-BLZ945: p = 0.2488, t = 1.594, df = 14; Control 3–4 weeks: p = 0.3715, t = 1.323, df = 14; BLZ945 3–4 weeks: p = 0.3717, t = 1.322, df = 14 |