Table 3

Descriptive statistics of immunostaining and confocal data

AnalysisMean ± SEMTreatment
IBA1 (two-way ANOVA, Sidak’s multiple comparisons test)4 weeks:
Control 0.06 ± 0.004 (n = 6), BLZ945 0.05 ± 0.01 (n = 6);
7 weeks:
Control 0.04 ± 0.01 (n = 4),
BLZ945 0.04 ± 0.003 (n = 7)
4-week Control-BLZ945:
p =0.0574, t =2.36, df = 19;
7-week Control-BLZ945:
p =0.9113, t =0.388, df = 19;
Control 4–7 weeks:
p =0.0166, t =2.943, df = 19;
BLZ945 4–7 weeks:
p =0.8425, t =0.529, df = 19
GFAP (two-way ANOVA, Sidak’s multiple comparisons test)4 weeks:
Control 0.05 ± 0.005 (n = 11, BLZ945 0.04 ± 0.005 (n = 6);
7 weeks:
Control 0.04 ± 0.003 (n = 6),
BLZ945 0.04 ± 0.002 (n = 8)
4-week Control-BLZ945:
p =0.0258, t =2.66, df = 27;
7-week Control-BLZ945:
p =0.8450, t =0.522, df = 27;
Control 4–7 weeks:
p =0.2535, t =1.537, df = 27;
BLZ945 4–7 weeks:
p =0.8381, t =0.534, df = 27
Calyx of Held surface area
(two-way ANOVA, Sidak’s multiple comparisons test)
3 weeks:
Control 687.5 ± 35.70 (n = 4), BLZ945 680.1.8 ± 13.40 (n = 4);
4 weeks:
Control 747.1 ± 21.19 (n = 5), BLZ945 707.8 ± 56.78 (n = 5)
3-week Control-BLZ945:
p =0.9895, t =0.1310, df = 14;
4-week Control-BLZ945:
p =0.7005, t =0.7724, df = 14;
Control 3–4 weeks:
p =0.4941, t =1.103, df = 14;
BLZ945 3–4 weeks:
p =0.8527, t =0.5126, df = 14
Polyinnervatioon of MNTB neurons
(two-way ANOVA, Sidak’s multiple comparisons test)
3 weeks:
Control 9.92 ± 6.75 (n = 4), BLZ945 38.78 ± 9.89 (n = 4);
4 weeks:
Control 5.08 ± 3.15 (n = 5), BLZ945 16.59 ± 5.82 (n = 5)
3-week Control-BLZ945:
p =0.0191, t =2.998, df = 14;
4-week Control-BLZ945:
p =0.3645, t =1.336, df = 14;
Control 3–4 weeks:
p =0.8435, t =0.53, df = 14;
BLZ945 3–4 weeks:
p =0.0574, t =2.43, df = 14
Calyceal shape factor analysis
(two-way ANOVA, Sidak’s multiple comparisons test)
3 weeks:
Control 6.394 ± 0.16 (n = 4), BLZ945 5.6 ± 0.17 (n = 4);
4 weeks:
Control 6.74 ± 0.13 (n = 5), BLZ945 6.35 ± 0.24 (n = 5)
3-week Control-BLZ945:
p =0.3210, t =1.425, df = 14;
4-week Control-BLZ945:
p =0.2488, t =1.594, df = 14;
Control 3–4 weeks:
p =0.3715, t =1.323, df = 14;
BLZ945 3–4 weeks:
p =0.3717, t =1.322, df = 14