Table 1

Summary of the experimental conditions and inclusion criteria used in published studies

PaperAnesthesiaIndicatorStimulusStimulus repetitionsResponsiveness criteriaPercentage of responsive cells# Cells Allen Brain Observatory
Study 1
Sun et al. (2016)
NoneGCaMP6s12-s full-field square grating
TF: 0.5, 1 Hz
SF 0.05cpd
8–16 directions
10Mean ΔF/F > 10%49% (n = 1279/2609)17.5% (n = 2962/16923)
Study 2
Roth et al. (2012)
UrethaneOGB-15-s full-field sine wave grating
TF: 0.5, 1, 2, 4 Hz
SF: 0.01, 0.02, 0.04, 0.08, 0.16cpd
8 directions
4In 50% of trials, mean ΔF/F >baseline + 3σ
Mean response > 5%
44% (n = 399/973)6.3% (n = 1068/16923)
Study 3
Andermann et al. (2011)
NoneGCaMP340° sine wave grating patches
TF: 0.5, 1, 2, 4, 8, 15, 24 Hz
SF: 0.02, 0.04, 0.08, 0.16, 0.32cpd
Direction: upward
9–28t test comparing grating response with blank sweep with Bonferroni correction (p < 0.05/n)8% (n = 28/340)29.6% (n = 5015/16923)
Study 4
Marshel et al. (2011)
IsofluoraneOGB-14-s full-field sine wave gratings
SF: 0.04 cpd
TF: 0.5,1, 2, 4, 8 Hz
8 directions
5Mean ΔF/F > 6%
Reliability >1(see Materials and Methods)
42% (n = 586/1395)7.8% (n = 1068/16923)
Study 5
Tohmi et al. (2014)
UrethaneFura-25-s ramping square and sine wave gratings
SF 0.05 and 0.1cpd
8 directions
28–124Max ΔF/F > 5%41.2% (n = 142/347)94.7 (n = 16,033/16,923)
• Last column shows the number of neurons selected from the Allen Brain Observatory.