Summary of the experimental conditions and inclusion criteria used in published studies
| Paper | Anesthesia | Indicator | Stimulus | Stimulus repetitions | Responsiveness criteria | Percentage of responsive cells | # Cells Allen Brain Observatory |
|---|---|---|---|---|---|---|---|
| Study 1 Sun et al. (2016) | None | GCaMP6s | 12-s full-field square grating TF: 0.5, 1 Hz SF 0.05cpd 8–16 directions | 10 | Mean ΔF/F > 10% | 49% (n = 1279/2609) | 17.5% (n = 2962/16923) |
| Study 2 Roth et al. (2012) | Urethane | OGB-1 | 5-s full-field sine wave grating TF: 0.5, 1, 2, 4 Hz SF: 0.01, 0.02, 0.04, 0.08, 0.16cpd 8 directions | 4 | In 50% of trials, mean ΔF/F >baseline + 3σ Mean response > 5% | 44% (n = 399/973) | 6.3% (n = 1068/16923) |
| Study 3 Andermann et al. (2011) | None | GCaMP3 | 40° sine wave grating patches TF: 0.5, 1, 2, 4, 8, 15, 24 Hz SF: 0.02, 0.04, 0.08, 0.16, 0.32cpd Direction: upward | 9–28 | t test comparing grating response with blank sweep with Bonferroni correction (p < 0.05/n) | 8% (n = 28/340) | 29.6% (n = 5015/16923) |
| Study 4 Marshel et al. (2011) | Isofluorane | OGB-1 | 4-s full-field sine wave gratings SF: 0.04 cpd TF: 0.5,1, 2, 4, 8 Hz 8 directions | 5 | Mean ΔF/F > 6% Reliability >1(see Materials and Methods) | 42% (n = 586/1395) | 7.8% (n = 1068/16923) |
| Study 5 Tohmi et al. (2014) | Urethane | Fura-2 | 5-s ramping square and sine wave gratings SF 0.05 and 0.1cpd 8 directions | 28–124 | Max ΔF/F > 5% | 41.2% (n = 142/347) | 94.7 (n = 16,033/16,923) |
Last column shows the number of neurons selected from the Allen Brain Observatory.