Table 2

Reagents and other materials

ProductVendorCatalog no.RRID
Neg-50ThermoScientific6502
Fluoromount GSouthernBiotech0100-01
DAPISigma-AldrichD9542-1MG
DMEMCorning10013-CV
Phenol red-free DMEMLife technologies31053-028
DMEM/F12Gibco11039-021
Mouse M-CSFShenandoah200-08
PercollGE Healthcare17-0891-01
PapainSigmaP3125
Dispase IIStemcell07913
DNase ISigmaD4263
Bovine serum albuminSigmaA7030
Fetal bovine aerumAtlanta BiologicalalalsS11150H
Penicillin/streptomycinCellgro30-008-CI
Poly-D-lysineSigmaP6407-5MG
PowerUP SYBR Green Master MixThermoFisherA25777
TRIzolLife Technologies15596018
Verso cDNA synthesis kitThermoFisherAB1453B
Pierce BCA Protein Assay kitThermoScientific23225
Chemiluminescent SubstrateThermoScientific34580
pHrodo Green Zymosan Bioparticles Conjugate for PhagocytosisThermoFisherP35365
Falcon 40 μm Cell StrainerCorning352340
Preseparation Filters (30 μm)Miltenyi130-041-407
MS ColumnsMiltenyi130-042-201
PilocarpineSigmaP6503