Primers used in this study
| Primer number | FP/RP | Primer sequence | Gene name | Vector backbone |
|---|---|---|---|---|
| NK181 NK196 | FP RP | atcagcatgcatcttgaaaacctcaatcgaaccag ctctctcccgggggacatatcttgaaagtttggaatggag | Psrx-97 | pPD49.26_mCherry |
| NK214 NK196 | FP RP | ctctctcgcatgcggtaagttttgcagtctaggcag ctctctcccgggggacatatcttgaaagtttggaatggag | Psrx-97 | pPD49.26_mCherry |
| NK259 NK260 | FP RP | ctctctgcatgcggaaccgtatttttgtgcaatagtcg ctctctcccggggcaagatgaaatttccaaaaaagtttattgatatgg | Posm-10 | pPD95.75 |
| NK262 NK263 | FP RP | ctctctgcatgcgccaaaactgctgaacttttg ctctctcccgggcttctgtagaaatttcaagactgatcac | Psrb-6 | pPD95.75 |
| NK197 NK279 | FP RP | ctctctcccgggatgtccttatcgaattggacgc ctctctggtaccttcaacatgatcctattcaagtttggtatttttc | srx-97_UTR | pPD49.26 |
| NK197 NK254 | FP RP | ctctctcccgggatgtccttatcgaattggacgc ctctctggtacctcaaaatgtgactgttaaaactgtgactt | srx-97 gene | pPD49.26_mCherry |
| gRNA_1 | atcaggtctcctcttccccacttatgactattacagttttagagctagaaatagcaag | srx-97 gene | pRB1017 | |
| gRNA_2 | atcaggtctcctcttaaaattataaggcgtaggcagttttagagctagaaatagcaag | srx-97 gene | pRB1017 | |
| Homology amr_1 | FP RP | atcatctagatcttccggacgtgaatcttctatc atcagcatgcatcttgaaaacctcaatcgaaccag | srx-97 gene | pPD95.75 |
| Homology amr_2 | FP RP | atcagggccccattgcacaactgataagatagtgc atcacttaagttcaacatgatcctattcaagtttggt | srx-97 gene | pPD95.75 |
| NK263 NK264 NK265 | EFP IFP RP | ctacagtttagtgcttgccacag tgtcgaaattaaagggtttcgagg gctgtccaacgcaattttcg | gpc-1 | genotyping |
| NK303 NK304 NK279 | EFP IFP RP | gctaggtggagggctgattg gctaggtggagggctgatta atgggcggtggaagttcg | osm-9 | genotyping |
| NK207 NK209 | FP RP | ggatagaagattcacgtccggaag tccatgtggggtttgctctg | srx-97 | genotyping |
| NK210 NK179 | FP RP | cttccaacatgaaaagcactatcttatcag ttcaacatgatcctattcaagtttggt | srx-97 | genotyping |
| PRS394 PRS395 PRS396 | FP ERP IRP | gcggttcggatacgaaaatacttg gacggagaagtgtatccgttatatc ccatgcgtccgtccctaatcc | tax-4 | genotyping |
| PRS442 PRS443 PRS444 | FP ERP IRP | cactggcgacgattgtcagatc gttatttccagtagatgtggccacg gtagccaaaatgagttgatctg | tax-2 | genotyping |
| AB37 AB38 AB39 | FP-WT FP-glr-1 RP | acctttcggctccgacttg acctttcggctccgactta attgaaatgaccataccacc | glr-1 | genotyping |
FP, forward primer; RP, reverse primer; FP-WT, external forward primer for WT glr-1 sequence; FP-glr-1, external forward primer for glr-1 sequence in the glr-1 point mutation line.