Table 1

Primers used in this study

Primer numberFP/RPPrimer sequenceGene nameVector backbone
NK181
NK196
FP
RP
atcagcatgcatcttgaaaacctcaatcgaaccag
ctctctcccgggggacatatcttgaaagtttggaatggag
Psrx-97pPD49.26_mCherry
NK214
NK196
FP
RP
ctctctcgcatgcggtaagttttgcagtctaggcag
ctctctcccgggggacatatcttgaaagtttggaatggag
Psrx-97pPD49.26_mCherry
NK259
NK260
FP
RP
ctctctgcatgcggaaccgtatttttgtgcaatagtcg
ctctctcccggggcaagatgaaatttccaaaaaagtttattgatatgg
Posm-10pPD95.75
NK262
NK263
FP
RP
ctctctgcatgcgccaaaactgctgaacttttg
ctctctcccgggcttctgtagaaatttcaagactgatcac
Psrb-6pPD95.75
NK197
NK279
FP
RP
ctctctcccgggatgtccttatcgaattggacgc
ctctctggtaccttcaacatgatcctattcaagtttggtatttttc
srx-97_UTRpPD49.26
NK197
NK254
FP
RP
ctctctcccgggatgtccttatcgaattggacgc
ctctctggtacctcaaaatgtgactgttaaaactgtgactt
srx-97 genepPD49.26_mCherry
gRNA_1atcaggtctcctcttccccacttatgactattacagttttagagctagaaatagcaagsrx-97 genepRB1017
gRNA_2atcaggtctcctcttaaaattataaggcgtaggcagttttagagctagaaatagcaagsrx-97 genepRB1017
Homology amr_1FP
RP
atcatctagatcttccggacgtgaatcttctatc
atcagcatgcatcttgaaaacctcaatcgaaccag
srx-97 genepPD95.75
Homology amr_2FP
RP
atcagggccccattgcacaactgataagatagtgc
atcacttaagttcaacatgatcctattcaagtttggt
srx-97 genepPD95.75
NK263
NK264
NK265
EFP
IFP
RP
ctacagtttagtgcttgccacag
tgtcgaaattaaagggtttcgagg
gctgtccaacgcaattttcg
gpc-1genotyping
NK303
NK304
NK279
EFP
IFP
RP
gctaggtggagggctgattg
gctaggtggagggctgatta
atgggcggtggaagttcg
osm-9genotyping
NK207
NK209
FP
RP
ggatagaagattcacgtccggaag
tccatgtggggtttgctctg
srx-97genotyping
NK210
NK179
FP
RP
cttccaacatgaaaagcactatcttatcag
ttcaacatgatcctattcaagtttggt
srx-97genotyping
PRS394
PRS395
PRS396
FP
ERP
IRP
gcggttcggatacgaaaatacttg
gacggagaagtgtatccgttatatc
ccatgcgtccgtccctaatcc
tax-4genotyping
PRS442
PRS443
PRS444
FP
ERP
IRP
cactggcgacgattgtcagatc
gttatttccagtagatgtggccacg
gtagccaaaatgagttgatctg
tax-2genotyping
AB37
AB38
AB39
FP-WT
FP-glr-1
RP
acctttcggctccgacttg 
acctttcggctccgactta 
attgaaatgaccataccacc 
glr-1genotyping
  • FP, forward primer; RP, reverse primer; FP-WT, external forward primer for WT glr-1 sequence; FP-glr-1, external forward primer for glr-1 sequence in the glr-1 point mutation line.