Data | Data structure | Type of test | Power | Mean ± SEM | Number of cells | |
---|---|---|---|---|---|---|
Control | Non-normal distribution | 1.00 ± 0.00 | 10 | |||
100 μm ML-9 | Normal distribution | 7.19 ± 1.32 | 10 | |||
a | Control vs ML-9 | Wilcoxon signed-rank test | p = 0.0020 | |||
0 Ca2+ (experimental condition 1) | Normal distribution | 0.28 ± 0.07 | 10 | |||
0 Ca2+ + ML-9 | Normal distribution | 3.35 ± 0.57 | 10 | |||
b | 0 Ca2+ vs 0 Ca2+ + ML-9 | paired t test | p = 0.0001 | |||
0 Ca2+ + BAPTA-AM (experimental condition 2) | Normal distribution | 0.28 ± 0.07 | 9 | |||
0 Ca2+ + BAPTA-AM + ML-9 | Non-normal distribution | 4.04 ± 0.85 | 9 | |||
c | 0 Ca2+ + BAPTA-AM vs 0 Ca2+ + BAPTA-AM + ML-9 | Wilcoxon signed-rank test | p = 0.0039 | |||
Relative effect of ML-9 under different conditions | ||||||
ML-9 effect under control condition | Normal distribution | 7.19 ± 1.32 | 10 | |||
ML-9 effect under experimental condition 1 | Non-normal distribution | 18.69 ± 4.64 | 10 | |||
ML-9 effect under experimental condition 2 | Non-normal distribution | 20.31 ± 5.12 | 9 | |||
d | ML-9 (experimental condition 1) vs ML-9 (control condition) | Mann–Whitney test | p = 0.0232 | |||
e | ML-9 (experimental condition 2) vs ML-9 (control condition) | Mann–Whitney test | p = 0.0030 |
0 Ca2+: remove extracellular Ca2+.