Data | Data structure | Type of test | Power | Mean ± SEM | Number of cells | |
---|---|---|---|---|---|---|

Control | Non-normal distribution | 1.00 ± 0.00 | 10 | |||

100 μm ML-9 | Normal distribution | 7.19 ± 1.32 | 10 | |||

a | Control vs ML-9 | Wilcoxon signed-rank test | p = 0.0020 | |||

0 Ca^{2+}(experimental condition 1) | Normal distribution | 0.28 ± 0.07 | 10 | |||

0 Ca^{2+} + ML-9 | Normal distribution | 3.35 ± 0.57 | 10 | |||

b | 0 Ca^{2+} vs 0 Ca^{2+} + ML-9 | paired t test | p = 0.0001 | |||

0 Ca^{2+} + BAPTA-AM(experimental condition 2) | Normal distribution | 0.28 ± 0.07 | 9 | |||

0 Ca^{2+} + BAPTA-AM + ML-9 | Non-normal distribution | 4.04 ± 0.85 | 9 | |||

c | 0 Ca^{2+} + BAPTA-AM vs0 Ca ^{2+} + BAPTA-AM + ML-9 | Wilcoxon signed-rank test | p = 0.0039 | |||

Relative effect of ML-9 under different conditions | ||||||

ML-9 effect under control condition | Normal distribution | 7.19 ± 1.32 | 10 | |||

ML-9 effect under experimental condition 1 | Non-normal distribution | 18.69 ± 4.64 | 10 | |||

ML-9 effect under experimental condition 2 | Non-normal distribution | 20.31 ± 5.12 | 9 | |||

d | ML-9 (experimental condition 1) vs ML-9 (control condition) | Mann–Whitney test | p = 0.0232 | |||

e | ML-9 (experimental condition 2) vs ML-9 (control condition) | Mann–Whitney test | p = 0.0030 |

0 Ca

^{2+}: remove extracellular Ca^{2+}.