Table 1

Settings for Gad2 quantification in Drd2^Pet1^-CKO tissue

Probe	S1	S2	Prominence	R²	RMSE	MAE
E2	0.25	1	175	0.8696	0.5957	0.2458
E7/8	0.5	1	100	0.9421	0.8054	0.35
Cre	0.5	1	100	0.9679	3.829	2.2244
Fev	0.25	2	75	0.9555	4.414	3.7047
Gad2	0.25	16	150	0.8568	2.804	1.7973

Summary of settings and performance in a linear regression for semi-automated protocol versus hand counts for each FISH probe.