Genotyping primers for Hh Tet-On system
| Transgenic line | Amplicon | Primers |
|---|---|---|
| Tg(GBS-ptch2:RTTA-HA)umz39Tg | RTTA-HA | Fw 5′-GAATTCACCATGTCTAGACTGGACA-3′ |
| Rv 5′-CTAACTGTCGACAGCGTAATCTGG-3′ | ||
| Tg(biTETRE:gli1,NLS-mCherry)umz42Tg | mCherry | Fw 5′-CCAAGCTGAAGGTGACCAAG-3′ |
| Rv 5′-CTTGTAGATGAACTCGCCGTC-3′ | ||
| Tg(biTETRE:gli2aDR,NLS-mCherry)umz43Tg | mCherry | Fw 5′-CCAAGCTGAAGGTGACCAAG-3′ |
| Rv 5′-CTTGTAGATGAACTCGCCGTC-3′ |