Table 2

Statistical analysis for the qPCR experiments

BV-2 qPCR (Fig. 1F)
Unpaired t test	Mean difference	95.00% CI of difference	Significant?	Summary	p value
DMEM vs DMEM/F12	–4.973 ± 0.4619	–6.256 to –3.691	Yes	***	0.0004
BV-2 siRNA qPCR (Fig. 1L)
Tukey's multiple comparisons test	Mean difference	95.00% CI of difference	Significant?	Summary	Adjusted p value
DMEM vs DMEM/F12	3.192	–0.3346 to 6.718	No	ns	0.0719	A-B
DMEM vs siFat3	–2.381	–5.907 to 1.146	No	ns	0.1763	A-C
DMEM/F12 vs siFat3	–5.572	–9.099 to –2.046	Yes	**	0.0068	B-C
BV-2 NEAAs metals others qPCR (Fig. 2B)
Dunnett's multiple comparisons test	Mean difference	95.00% CI of difference	Significant?	Summary	Adjusted p value	A-?
DMEM vs DMEM/F12	2.113	1.032 to 3.195	Yes	***	0.0007	B	DMEM/F12
DMEM vs NEAAs	0.5533	–0.5281 to 1.635	No	ns	0.4259	C	NEAAs
DMEM vs metals	0.4133	–0.6681 to 1.495	No	ns	0.6518	D	Metals
DMEM vs others	2.113	1.032 to 3.195	Yes	***	0.0007	E	Others
BV-2 others qPCR (Fig. 2C)
Dunnett's multiple comparisons test	Mean difference	95.00% CI of difference	Significant?	Summary	Adjusted p value	C-?
DMEM vs DMEM/F12	2.858	1.779 to 3.936	Yes	****	<0.0001	A	DMEM/F12
DMEM vs HYP	6.176	5.098 to 7.255	Yes	****	<0.0001	B	HYP
DMEM vs biotin	–0.5829	–1.661 to 0.4955	No	ns	0.4517	D	Biotin
DMEM vs linoleic	1.584	0.5057 to 2.663	Yes	**	0.0038	E	Linoleic
DMEM vs VB12	–0.9733	–2.052 to 0.1052	No	ns	0.0839	F	VB12
DMEM vs lipoic	–0.6831	–1.761 to 0.3954	No	ns	0.3094	G	Lipoic
Primary microglia qPCR (Figure 2I)
Dunnett's multiple comparisons test	Mean difference	95.00% CI of difference	Significant?	Summary	Adjusted p value	A-?
DMEM vs DMEM/F12	1.35	0.629 to 2.06	Yes	**	0.003	B	DMEM/F12
DMEM vs HYP	1.15	0.436 to 1.87	Yes	**	0.007	C	HYP