Table 3

Details of double-label in situ hybridization analyses for ENaCα with taste cell marker genes

Tissue	Mouse	Marker gene	No. of taste buds	Ratio (%)*
FuP	B6, n = 3	Skn-1a	40	12.8 ± 2.2
	B6, n = 3	Calhm3	25	13.9 ± 3.2
	B6, n = 3	Trpm5	27	0
	B6, n = 3	Pkd2l1	20	38.0 ± 7.2
	Skn-1a^–/–, n = 3	Pkd2l1	27	7.2 ± 2.0
Palate	B6, n = 3	Skn-1a	32	13.0 ± 4.7
	B6, n = 3	Calhm3	28	8.8 ± 0.6
	B6, n = 3	Trpm5	25	0
	B6, n = 3	Pkd2l1	22	44.5 ± 3.0
	Skn-1a^–/–, n = 3	Pkd2l1	39	2.9 ± 1.7

Double-positive cells to marker gene-positive cells (Skn-1a, Calhm3, and Trpm5) or to Pkd2l1^–ENaCα⁺ cells to ENaCα⁺ cells.