Table 1

Data analysis estimation statistics

Figure	Plot	Analysis	Control group	Test group	Control_N	Test_N	Effect_size	Is_paired	Difference	ci_width	ci_lower_limit	ci_upper_limit
Figure 2B	Multiple two groups	Analysis #1	CV/VEH	CV/CNO	7	7	Mean difference	False	–11.97857143	95.00%	–55.23714286	33.12428571
Figure 2B	Multiple two groups	Analysis #1	M4/VEH	M4/CNO	6	6	Mean difference	False	–41.95	95.00%	–55.33333333	–26.9
Figure 2C	Multiple two groups	Analysis #2	CV/VEH	CV/CNO	7	7	Mean difference	False	–2.857142857	95.00%	–19.57142857	17.71428571
Figure 2C	Multiple two groups	Analysis #2	M4/VEH	M4/CNO	6	6	Mean difference	False	–20.5	95.00%	–35.33333333	–12.33333333
Figure 2D	Multiple two groups	Analysis #3	CV/VEH	CV/CNO	6	5	Mean difference	False	–33	95.00%	–81.66666667	13.33333333
Figure 2D	Multiple two groups	Analysis #3	M4/VEH	M4/CNO	10	10	Mean difference	False	121.05	95.00%	31.25	188.4
Figure 3A	Two groups	Analysis #4	VEH	CNO	10	12	Mean difference	False	0.1358	95.00%	–11.80348333	14.73156667
Figure 3B	Paired	Analysis #5	VEH	CNO	7	7	Mean difference	True	–2.714285714	95.00%	–47.14285714	46.14285714
Figure 3C	Paired	Analysis #6	VEH	CNO	7	7	Mean difference	True	10.41428571	95.00%	–115.8285714	139.0714286
Figure 3D	Paired	Analysis #7	VEH	CNO	7	7	Mean difference	True	6.857142857	95.00%	2.285714286	10.57142857
Figure 3E	Paired	Analysis #8	VEH	CNO	6	6	Mean difference	True	1	95.00%	–2.166666667	4
Figure 3F	Paired	Analysis #9	VEH	CNO	6	6	Mean difference	True	0.5	95.00%	–0.666666667	2.166666667
Figure 3G	Multiple paired	Analysis #10	VEH/Active	VEH/Inactive	7	7	Mean difference	True	–432.2857143	95.00%	–595	–346.2857143
Figure 3G	Multiple paired	Analysis #10	CNO/Active	CNO/Inactive	7	7	Mean difference	True	–450.1428571	95.00%	–529.4285714	–376.8571429
Figure 3H	Multiple paired	Analysis #11	VEH/Active	VEH/Inactive	6	6	Mean difference	True	–46.66666667	95.00%	–56.33333333	–34.5
Figure 3H	Multiple paired	Analysis #11	CNO/Active	CNO/Inactive	6	6	Mean difference	True	–42.66666667	95.00%	–69.5	–21.5
Figure 3I	Multiple paired	Analysis #12	VEH/Active	VEH/Inactive	6	6	Mean difference	True	–16	95.00%	–25.66666667	–9.833333333
Figure 3I	Multiple paired	Analysis #12	CNO/Active	CNO/Inactive	6	6	Mean difference	True	–21.16666667	95.00%	–26.66666667	–15.66666667
Figure 4B	Two groups	Analysis #13	VEH	CNO	5	6	Mean difference	False	–71.33333333	95.00%	–84.3	–56.4
Figure 5C	Multiple two groups	Analysis #14	VEH/SAL	VEH/DOI	4	5	Mean difference	False	1.9391	95.00%	–11.8578	12.1919
Figure 5C	Multiple two groups	Analysis #14	CNO/SAL	CNO/DOI	4	5	Mean difference	False	2.87635	95.00%	–7.7447	17.15425
Figure 5D	Multiple two groups	Analysis #15	VEH/SAL	VEH/DOI	4	5	Mean difference	False	–11.7635	95.00%	–29.804	15.1315
Figure 5D	Multiple two groups	Analysis #15	CNO/SAL	CNO/DOI	6	5	Mean difference	False	35.83333333	95.00%	11.43333333	55.36666667
Figure 5E	Multiple two groups	Analysis #16	VEH/SAL	VEH/DOI	4	5	Mean difference	False	2.45	95.00%	–0.9	6.25
Figure 5E	Multiple two groups	Analysis #16	CNO/SAL	CNO/DOI	6	5	Mean difference	False	9.933333333	95.00%	–2.166666667	17.56666667
Extended Data Figure 3-1	Two groups	Analysis #17	VEH	CNO	10	12	Mean difference	False	–29.94166667	95.00%	–99.88833333	17.455
Extended Data Figure 5-1	Multiple two groups	Analysis #18	VEH/SAL	VEH/DOI	4	5	Mean difference	False	4.23	95.00%	–14.915	25.525
Extended Data Figure 5-1	Multiple two groups	Analysis #18	CNO/SAL	CNO/DOI	4	5	Mean difference	False	1.545	95.00%	–28.445	22.1

VEH, peripheral vehicle injection; CNO: peripheral CNO injection; SAL, saline microinjection through cannula; DOI, DOI microinjection through cannula; M4, hM4Di expressing mice; CV, control vector expressing mice; active, active lever presses; inactive, inactive lever presses.