Table 2

Statistical analysis of experimental results

ExperimentData structureType of testTest statisticp value
Immunostaining
    1aTd-Tomato/PV-immunostain
colocalization
Normal
(SW and DP)
Two-tailed unpaired
t test
t = 0.065
df = 4.42
p = 0.95
    1bPV-immunostained cells onlyNormal
(SW and DP)
Two-tailed unpaired
t test
t = 0.721
df = 5
p = 0.47
    1cTd-Tomato cells onlyNormal
(SW and DP)
Two-tailed unpaired
t test
t = 0.774
df = 5
p = 0.56
Cell counts
    2aTotal DGNormal
(SW and DP)
Two-tailed unpaired
t test
t = 1.971
df = 15
p = 0.07
    2bHilusNormal
(SW and DP)
Two-tailed unpaired
t test
t = 2.431
df = 15
p = 0.03
    2cSubgranular zoneNormal
(SW and DP)
Two-tailed unpaired
t test
t = 1.579
df = 15
p = 0.14
    2dGranule cell layerNormal
(SW)
Two-tailed unpaired
t test
t = 0.6157
df = 15
p = 0.55
    2eMolecular layerNormal
(SW and DP)
Two-tailed unpaired
t test
t = 0.9811
df = 15
p = 0.34
    2fSuprapyramidal bladeNormal
(SW and DP)
Two-tailed unpaired
t test
t = 2.048
df = 15
p = 0.06
    2gInfrapyramidal bladeNormal
(SW and DP)
Two-tailed unpaired
t test
t = 1.117
df = 15
p = 0.28
    2fSeptotemporalNormal (DP)Two-way
ANOVA
F(1,87) = 0.87p = 0.35
Intrinsic properties
    3afEPSPsNormal
(SW and DP)
Repeated measures
ANOVA
F(19,190) = 14.34p < 0.0001
    3bInput resistanceNormal (DP)Two-tailed unpaired
t test
t = 0.3717
df = 18
p = 0.71
    3cResting membrane potentialNormal
(SW and DP)
Two-tailed unpaired
t test
t = 1.279
df = 18
p = 0.21
    3dAP thresholdNormal (SW)Two-tailed unpaired
t test
t = 1.244
df = 18
p = 0.23
    3eAP firing frequencyNormal (SW)Repeated measures
ANOVA
F(1,13) = 0.422p = 0.53
    3fAP half-widthNormal (SW)Two-tailed unpaired
t test
t = 0.612
df = 14
p = 0.55
mEPSCs
    4aInterevent intervalNon-normal
(SW and DP)
K-S testD = 0.162p < 0.0001
    4bAmplitudeNon-normal
(SW and DP)
K-S testD = 0.113p = 0.006
    4cRise τNon-normal
(SW and DP)
K-S testD = 0.104p = 0.004
    4dDecay τNon-normal
(SW and DP)
K-S testD = 0.072p = 0.20
eEPSCs
    5aSlopeNormal
(SW and DP)
Two-way ANOVAF(1,13) = 0.1825p = 0.68
    5bAmplitudeNormal
(SW and DP)
Two-way ANOVAF(1,22) = 4.715p = 0.04
    5cCharge transferNormal
(SW and DP)
Two-way ANOVAF(1,21) = 5.426p = 0.03
    5dSlope
(BMI wash-in)
Normal
(SW and DP)
Two-way ANOVAF(1,10) = 0.005518p = 0.94
    5eAmplitude
(BMI wash-in)
Normal
(SW and DP)
Two-way ANOVAF(1,10) = 0.8552p = 0.38
    5fCharge transfer (BMI wash-in)Normal
(SW and DP)
Two-way ANOVAF(1,10) = 0.9528p = 0.35
mIPSCs
    6aAmplitudeNon-normal
(SW and DP)
K-S testD = 0.134p = 0.0003
    6bRise τNon-normal
(SW and DP)
K-S testD = 0.126p = 0.008
    6cInterevent intervalNon-normal
(SW and DP)
K-S testD = 0.064p = 0.25
    6dDecay τNon-normal
(SW and DP)
K-S testD = 0.038p = 0.86
Evoked APs
    7aResting membrane potentialNormal (SW)Welch’s unpaired
t test
t = 0.9494
df = 8
p = 0.37
    7bWhole-cell capacitanceNormal (SW)Welch’s unpaired
t test
t = 0.4834
df = 8
p = 0.64
    7cEvoked APs (normal ACSF)Normal (SW)Welch’s unpaired
t test
t = 9.084
df = 4
p = 0.0008
    7dEvoked APs
(sham pre-PCTX and post-PCTX)
Normal (SW)Paired t testt = 11.27,
df = 4
p = 0.0004
    7eEvoked APs
(LFPI pre-PCTX and post-PCTX)
Normal (SW)Paired t testt = 5.119
df = 4
p = 0.0034
    9fEvoked APs (100 nm PCTX)Normal (SW)Welch’s unpaired
t test
t = 1.138
df = 6
p = 0.29
  • For each experiment (second column), the statistical results included the structure of the data distribution as determined by Shapiro–Wilk (SW) or D’Agostino–Pearson (DP) normality tests (third column), the statistical test used, the test statistic and degrees of freedom (df), and the p value; p values >0.05 are rounded up to two decimal places. DG, dentate gyrus; PCTX, picrotoxin.