Table 2

Quantitative analysis of axonal KIF1A-S402A and KIF1A-S402E transport in tau+/+ neurons

Traffic values
All eventsAnterogradeRetrograde
Flux (min–1)   
 WT8.00 ± 0.466.11 ± 0.301.88 ± 0.30
 WT + ABO3.05 ± 0.46****#### 1.94 ± 0.20****#### 1.12 ± 0.20####
 S402A7.72 ± 0.345.10 ± 0.402.71 ± 0.27
 S402A + ABO3.80 ± 0.24****#### 2.04 ± 0.18****#### 2.71 ± 0.27#
 S402E7.63 ± 0.426.30 ± 0.371.34 ± 0.17##
Velocity (μm/s)   
 WT2.05 ± 0.112.28 ± 0.131.53 ± 0.14
 WT + ABO1.72 ± 0.141.81 ± 0.14* 1.55 ± 0.15
 S402A2.03 ± 0.072.08 ± 0.061.96 ± 0.15
 S402A + ABO1.23 ± 0.10****####$$ 1.35 ± 0.12****###$ 1.13 ± 0.11###
 S402E2.03 ± 0.102.23 ± 0.121.51 ± 0.12
Run length (μm)   
 WT9.14 ± 1.0210.74 ± 1.365.64 ± 0.57
 WT + ABO6.26 ± 0.62* 6.62 ± 0.68* 5.64 ± 0.89
 S402A7.34 ± 0.909.03 ± 1.425.31 ± 0.39
 S402A + ABO4.77 ± 0.29*** 4.93 ± 0.42***# 4.53 ± 0.25
 S402E9.09 ± 0.9010.46 ± 1.085.31 ± 0.54
  • WT: n = 16 kymographs (16 cells, 789 puncta).

  • S402A: n = 16 kymographs (16 cells, 829 puncta).

  • WT + ABO: n = 18 kymographs (18 cells, 381 puncta).

  • S402A + ABO: n = 18 kymographs (18 cells, 430 puncta).

  • S402E: n = 16 kymographs (16 cells, 743 puncta).

  • *p < 0.05, when compared with WT.

  • **p < 0.01, when compared with WT.

  • ***p < 0.001, when compared with WT.

  • ****p < 0.0001, when compared with WT.

  • #p < 0.05, when compared with S402A.

  • ##p < 0.01, when compared with S402A.

  • ###p < 0.001, when compared with S402A.

  • ####p < 0.0001, when compared with S402A.

  • $p < 0.05, when comparing AβO-treated conditions (significance denoted in S402 + AβO condition).

  • $$p < 0.01, when comparing AβO-treated conditions (significance denoted in S402 + AβO condition).

  • $$$p < 0.001, when comparing AβO-treated conditions (significance denoted in S402 + AβO condition).

  • $$$$p < 0.0001, when comparing AβO-treated conditions (significance denoted in S402 + AβO condition).