Quantitative analysis of axonal KIF1A-S402A and KIF1A-S402E transport in tau+/+ neurons
Traffic values | |||
---|---|---|---|
All events | Anterograde | Retrograde | |
Flux (min–1) | |||
WT | 8.00 ± 0.46 | 6.11 ± 0.30 | 1.88 ± 0.30 |
WT + ABO | 3.05 ± 0.46****#### | 1.94 ± 0.20****#### | 1.12 ± 0.20#### |
S402A | 7.72 ± 0.34 | 5.10 ± 0.40 | 2.71 ± 0.27 |
S402A + ABO | 3.80 ± 0.24****#### | 2.04 ± 0.18****#### | 2.71 ± 0.27# |
S402E | 7.63 ± 0.42 | 6.30 ± 0.37 | 1.34 ± 0.17## |
Velocity (μm/s) | |||
WT | 2.05 ± 0.11 | 2.28 ± 0.13 | 1.53 ± 0.14 |
WT + ABO | 1.72 ± 0.14 | 1.81 ± 0.14* | 1.55 ± 0.15 |
S402A | 2.03 ± 0.07 | 2.08 ± 0.06 | 1.96 ± 0.15 |
S402A + ABO | 1.23 ± 0.10****####$$ | 1.35 ± 0.12****###$ | 1.13 ± 0.11### |
S402E | 2.03 ± 0.10 | 2.23 ± 0.12 | 1.51 ± 0.12 |
Run length (μm) | |||
WT | 9.14 ± 1.02 | 10.74 ± 1.36 | 5.64 ± 0.57 |
WT + ABO | 6.26 ± 0.62* | 6.62 ± 0.68* | 5.64 ± 0.89 |
S402A | 7.34 ± 0.90 | 9.03 ± 1.42 | 5.31 ± 0.39 |
S402A + ABO | 4.77 ± 0.29*** | 4.93 ± 0.42***# | 4.53 ± 0.25 |
S402E | 9.09 ± 0.90 | 10.46 ± 1.08 | 5.31 ± 0.54 |
WT: n = 16 kymographs (16 cells, 789 puncta).
S402A: n = 16 kymographs (16 cells, 829 puncta).
WT + ABO: n = 18 kymographs (18 cells, 381 puncta).
S402A + ABO: n = 18 kymographs (18 cells, 430 puncta).
S402E: n = 16 kymographs (16 cells, 743 puncta).
*p < 0.05, when compared with WT.
**p < 0.01, when compared with WT.
***p < 0.001, when compared with WT.
****p < 0.0001, when compared with WT.
#p < 0.05, when compared with S402A.
##p < 0.01, when compared with S402A.
###p < 0.001, when compared with S402A.
####p < 0.0001, when compared with S402A.
$p < 0.05, when comparing AβO-treated conditions (significance denoted in S402 + AβO condition).
$$p < 0.01, when comparing AβO-treated conditions (significance denoted in S402 + AβO condition).
$$$p < 0.001, when comparing AβO-treated conditions (significance denoted in S402 + AβO condition).
$$$$p < 0.0001, when comparing AβO-treated conditions (significance denoted in S402 + AβO condition).