Table 1

Stats table

FigureFigure panelStatistical testConditionsResults
2 C Two-way ANOVA interactionF(1,35) = 0.016
p =0.90
Two-way ANOVA sex factorF(1,35) = 0.02
p =0.89
Two-way ANOVA drug factorF(1,35) = 23.9
p =0.00002
Two-way ANOVA means comparisonMale mice (CPP, saline vs nicotine)p =0.0011
Two-way ANOVA means comparisonFemale mice (CPP, saline vs nicotine)p =0.0064
D1 Two-way ANOVA interactionα4α6* nAChR upregulationF(1,23) = 0.45
p =0.51
Two-way ANOVA sex factorα4α6* nAChR upregulationF(1,25) = 0.12
p =0.73
Two-way ANOVA drug factorα4α6* nAChR upregulationF(1,25) = 19.0
p =0.0002
Two-way ANOVA means comparisonMale mice (α4α6* RID, saline vs nicotine)p =0.019
Two-way ANOVA means comparisonFemale mice (α4α6* RID, saline vs nicotine)p =0.0054
D2 Two-way ANOVA interactionα4* nAChR upregulationF(1,25) = 0.74
p =0.40
Two-way ANOVA sex factorα4* nAChR upregulationF(1,25) = 0.30
p =0.59
Two-way ANOVA drug factorα4* nAChR upregulationF(1,25) = 15.5
p =0.0005
Two-way ANOVA means comparisonMale mice (α4* RID, saline vs nicotine)p =0.05
Two-way ANOVA means comparisonFemale mice (α4* RID, saline vs nicotine)p =0.008
D3 Two-way ANOVA interactionα6* nAChR upregulationF(1,25) = 0.32
p =0.58
Two-way ANOVA sex factorα6* nAChR upregulationF(1,25) = 0.87
p =0.36
Two-way ANOVA drug factorα6* nAChR upregulationF(1,25) = 0.086
p =0.77
Two-way ANOVA means comparisonMale mice (α6* RID, saline vs nicotine)p =0.99
Two-way ANOVA means comparisonFemale mice (α6* RID, saline vs nicotine)p =0.99
3 A2 Linear regressionMale mice, CPP score vs VTA pDA α4α6* nAChR upregulationR2 = 0.67
F(1,5) = 10.1
p =0.025
A3 Linear regressionMale mice, CPP score vs VTA pDA α4* nAChR upregulationR2 = 0.83
F(1,5) = 24.4
p =0.004
A4 Linear regressionMale mice, CPP score vs VTA pDA α6* nAChR upregulationR2 = 0.74
F(1,5) = 14.3
p =0.013
B1 Linear regressionFemale mice, CPP score vs VTA pDA α4α6* nAChR upregulationR2 = 0.82
F(1,6) = 28.2
p =0.002
B2 Linear regressionFemale mice, CPP score vs VTA pDA α4* nAChR upregulationR2 = 0.81
F(1,6) = 25.6
p =0.002
B3 Linear regressionFemale mice, CPP score vs VTA pDA α6* nAChR upregulationR2 = 0.07
F(1,6) = 0.47
p =0.52
C2 Linear regressionCPP score vs VTA pDA α4α6* nAChR upregulationR2 = 0.08
F(1,3) = 0.24
p =0.65
C3 Linear regressionCPP score vs VTA pDA α4* nAChR upregulationR2 = 0.08
F(1,3) = 0.26
p =0.64
C4 Linear regressionCPP score vs VTA pDA α6* nAChR upregulationR2 < 0.01
F(1,3) = 0.01
p =0.94
4 A2 Two-way ANOVA interactionα4* nAChR upregulationF(1,27) = 0.99
p =0.33
Two-way ANOVA sex factorα4* nAChR upregulationF(1,27) = 2.86
p =0.10
Two-way ANOVA drug factorα4* nAChR upregulationF(1,27) = 17.1
p =0.0003
Two-way ANOVA means comparisonMale mice (α4* RID, saline vs nicotine)p =0.0012
Two-way ANOVA means comparisonFemale mice (α4* RID, saline vs nicotine)p =0.05
A3 Linear regressionMale CPP score vs SNr GABA α4* nAChR upregulationR2 = 0.55
F(1,6) = 7.39
p =0.04
A4 Linear regressionFemale CPP score vs SNr GABA α4* nAChR upregulationR2 = 0.56
F(1,6) = 6.45
p =0.05
B2 Two-way ANOVA interactionα4* nAChR upregulationF(1,24) = 0.43
p =0.52
Two-way ANOVA sex factorα4* nAChR upregulationF(1,24) = 0.38
p =0.55
Two-way ANOVA drug factorα4* nAChR upregulationF(1,24) = 14.0
p =0.001
Two-way ANOVA means comparisonMale mice (α4* RID, saline vs nicotine)p =0.04
Two-way ANOVA means comparisonFemale mice (α4* RID, saline vs nicotine)p =0.013
B3 Linear regressionMale CPP score vs SNr GABA α4* nAChR upregulationR2 < 0.001
F(1,6) < 0.001
p =0.99
B4 Linear regressionFemale CPP score vs SNr GABA α4* nAChR upregulationR2 = 0.075
F(1,6) = 0.40
p =0.55
D1 Unpaired t testDentate Gyrus α4* nAChR upregulation, saline vs nicotinep =0.047
D2 Linear regressionCPP score vs dentate gyrus α4* nAChR upregulationR2 = 0.02
F(1,4) = 0.09
p =0.78
5 C Unpaired t testCPP score, saline vs nicotinep =0.026
E Unpaired t testVTA pDA neuron firing frequency, saline vs nicotinep =0.0094
F Linear regressionCPP Score vs VTA pDA neuron firing frequencyR2 = 0.65
F(1,16) = 11.2
p =0.016
6 D Unpaired t testVTA pGABA neuron firing frequency, saline vs nicotinep =0.0056
E Linear regressionCPP Score vs VTA pGABA neuron firing frequencyR2 = 0.77
F(1,16) = 20.3
p =0.004