Stats table
| Figure | Figure panel | Statistical test | Conditions | Results |
|---|---|---|---|---|
| 2 | C | Two-way ANOVA interaction | F(1,35) = 0.016 p = 0.90 | |
| Two-way ANOVA sex factor | F(1,35) = 0.02 p = 0.89 | |||
| Two-way ANOVA drug factor | F(1,35) = 23.9 p = 0.00002 | |||
| Two-way ANOVA means comparison | Male mice (CPP, saline vs nicotine) | p = 0.0011 | ||
| Two-way ANOVA means comparison | Female mice (CPP, saline vs nicotine) | p = 0.0064 | ||
| D1 | Two-way ANOVA interaction | α4α6* nAChR upregulation | F(1,23) = 0.45 p = 0.51 | |
| Two-way ANOVA sex factor | α4α6* nAChR upregulation | F(1,25) = 0.12 p = 0.73 | ||
| Two-way ANOVA drug factor | α4α6* nAChR upregulation | F(1,25) = 19.0 p = 0.0002 | ||
| Two-way ANOVA means comparison | Male mice (α4α6* RID, saline vs nicotine) | p = 0.019 | ||
| Two-way ANOVA means comparison | Female mice (α4α6* RID, saline vs nicotine) | p = 0.0054 | ||
| D2 | Two-way ANOVA interaction | α4* nAChR upregulation | F(1,25) = 0.74 p = 0.40 | |
| Two-way ANOVA sex factor | α4* nAChR upregulation | F(1,25) = 0.30 p = 0.59 | ||
| Two-way ANOVA drug factor | α4* nAChR upregulation | F(1,25) = 15.5 p = 0.0005 | ||
| Two-way ANOVA means comparison | Male mice (α4* RID, saline vs nicotine) | p = 0.05 | ||
| Two-way ANOVA means comparison | Female mice (α4* RID, saline vs nicotine) | p = 0.008 | ||
| D3 | Two-way ANOVA interaction | α6* nAChR upregulation | F(1,25) = 0.32 p = 0.58 | |
| Two-way ANOVA sex factor | α6* nAChR upregulation | F(1,25) = 0.87 p = 0.36 | ||
| Two-way ANOVA drug factor | α6* nAChR upregulation | F(1,25) = 0.086 p = 0.77 | ||
| Two-way ANOVA means comparison | Male mice (α6* RID, saline vs nicotine) | p = 0.99 | ||
| Two-way ANOVA means comparison | Female mice (α6* RID, saline vs nicotine) | p = 0.99 | ||
| 3 | A2 | Linear regression | Male mice, CPP score vs VTA pDA α4α6* nAChR upregulation | R2 = 0.67 F(1,5) = 10.1 p = 0.025 |
| A3 | Linear regression | Male mice, CPP score vs VTA pDA α4* nAChR upregulation | R2 = 0.83 F(1,5) = 24.4 p = 0.004 | |
| A4 | Linear regression | Male mice, CPP score vs VTA pDA α6* nAChR upregulation | R2 = 0.74 F(1,5) = 14.3 p = 0.013 | |
| B1 | Linear regression | Female mice, CPP score vs VTA pDA α4α6* nAChR upregulation | R2 = 0.82 F(1,6) = 28.2 p = 0.002 | |
| B2 | Linear regression | Female mice, CPP score vs VTA pDA α4* nAChR upregulation | R2 = 0.81 F(1,6) = 25.6 p = 0.002 | |
| B3 | Linear regression | Female mice, CPP score vs VTA pDA α6* nAChR upregulation | R2 = 0.07 F(1,6) = 0.47 p = 0.52 | |
| C2 | Linear regression | CPP score vs VTA pDA α4α6* nAChR upregulation | R2 = 0.08 F(1,3) = 0.24 p = 0.65 | |
| C3 | Linear regression | CPP score vs VTA pDA α4* nAChR upregulation | R2 = 0.08 F(1,3) = 0.26 p = 0.64 | |
| C4 | Linear regression | CPP score vs VTA pDA α6* nAChR upregulation | R2 < 0.01 F(1,3) = 0.01 p = 0.94 | |
| 4 | A2 | Two-way ANOVA interaction | α4* nAChR upregulation | F(1,27) = 0.99 p = 0.33 |
| Two-way ANOVA sex factor | α4* nAChR upregulation | F(1,27) = 2.86 p = 0.10 | ||
| Two-way ANOVA drug factor | α4* nAChR upregulation | F(1,27) = 17.1 p = 0.0003 | ||
| Two-way ANOVA means comparison | Male mice (α4* RID, saline vs nicotine) | p = 0.0012 | ||
| Two-way ANOVA means comparison | Female mice (α4* RID, saline vs nicotine) | p = 0.05 | ||
| A3 | Linear regression | Male CPP score vs SNr GABA α4* nAChR upregulation | R2 = 0.55 F(1,6) = 7.39 p = 0.04 | |
| A4 | Linear regression | Female CPP score vs SNr GABA α4* nAChR upregulation | R2 = 0.56 F(1,6) = 6.45 p = 0.05 | |
| B2 | Two-way ANOVA interaction | α4* nAChR upregulation | F(1,24) = 0.43 p = 0.52 | |
| Two-way ANOVA sex factor | α4* nAChR upregulation | F(1,24) = 0.38 p = 0.55 | ||
| Two-way ANOVA drug factor | α4* nAChR upregulation | F(1,24) = 14.0 p = 0.001 | ||
| Two-way ANOVA means comparison | Male mice (α4* RID, saline vs nicotine) | p = 0.04 | ||
| Two-way ANOVA means comparison | Female mice (α4* RID, saline vs nicotine) | p = 0.013 | ||
| B3 | Linear regression | Male CPP score vs SNr GABA α4* nAChR upregulation | R2 < 0.001 F(1,6) < 0.001 p = 0.99 | |
| B4 | Linear regression | Female CPP score vs SNr GABA α4* nAChR upregulation | R2 = 0.075 F(1,6) = 0.40 p = 0.55 | |
| D1 | Unpaired t test | Dentate Gyrus α4* nAChR upregulation, saline vs nicotine | p = 0.047 | |
| D2 | Linear regression | CPP score vs dentate gyrus α4* nAChR upregulation | R2 = 0.02 F(1,4) = 0.09 p = 0.78 | |
| 5 | C | Unpaired t test | CPP score, saline vs nicotine | p = 0.026 |
| E | Unpaired t test | VTA pDA neuron firing frequency, saline vs nicotine | p = 0.0094 | |
| F | Linear regression | CPP Score vs VTA pDA neuron firing frequency | R2 = 0.65 F(1,16) = 11.2 p = 0.016 | |
| 6 | D | Unpaired t test | VTA pGABA neuron firing frequency, saline vs nicotine | p = 0.0056 |
| E | Linear regression | CPP Score vs VTA pGABA neuron firing frequency | R2 = 0.77 F(1,16) = 20.3 p = 0.004 |