Figure | Figure panel | Statistical test | Conditions | Results |
---|---|---|---|---|

2 |
C
| Two-way ANOVA interaction | F_{(1,35)} = 0.016p = 0.90 | |

Two-way ANOVA sex factor | F_{(1,35)} = 0.02p = 0.89 | |||

Two-way ANOVA drug factor | F_{(1,35)} = 23.9p = 0.00002 | |||

Two-way ANOVA means comparison | Male mice (CPP, saline vs nicotine) | p = 0.0011 | ||

Two-way ANOVA means comparison | Female mice (CPP, saline vs nicotine) | p = 0.0064 | ||

D
_{1} | Two-way ANOVA interaction | α4α6* nAChR upregulation | F_{(1,23)} = 0.45p = 0.51 | |

Two-way ANOVA sex factor | α4α6* nAChR upregulation | F_{(1,25)} = 0.12p = 0.73 | ||

Two-way ANOVA drug factor | α4α6* nAChR upregulation | F_{(1,25)} = 19.0p = 0.0002 | ||

Two-way ANOVA means comparison | Male mice (α4α6* RID, saline vs nicotine) | p = 0.019 | ||

Two-way ANOVA means comparison | Female mice (α4α6* RID, saline vs nicotine) | p = 0.0054 | ||

D
_{2} | Two-way ANOVA interaction | α4* nAChR upregulation | F_{(1,25)} = 0.74p = 0.40 | |

Two-way ANOVA sex factor | α4* nAChR upregulation | F_{(1,25)} = 0.30p = 0.59 | ||

Two-way ANOVA drug factor | α4* nAChR upregulation | F_{(1,25)} = 15.5p = 0.0005 | ||

Two-way ANOVA means comparison | Male mice (α4* RID, saline vs nicotine) | p = 0.05 | ||

Two-way ANOVA means comparison | Female mice (α4* RID, saline vs nicotine) | p = 0.008 | ||

D
_{3} | Two-way ANOVA interaction | α6* nAChR upregulation | F_{(1,25)} = 0.32p = 0.58 | |

Two-way ANOVA sex factor | α6* nAChR upregulation | F_{(1,25)} = 0.87p = 0.36 | ||

Two-way ANOVA drug factor | α6* nAChR upregulation | F_{(1,25)} = 0.086p = 0.77 | ||

Two-way ANOVA means comparison | Male mice (α6* RID, saline vs nicotine) | p = 0.99 | ||

Two-way ANOVA means comparison | Female mice (α6* RID, saline vs nicotine) | p = 0.99 | ||

3 |
A
_{2} | Linear regression | Male mice, CPP score vs VTA pDA α4α6* nAChR upregulation | R^{2} = 0.67F_{(1,5)} = 10.1p = 0.025 |

A
_{3} | Linear regression | Male mice, CPP score vs VTA pDA α4* nAChR upregulation | R^{2} = 0.83F_{(1,5)} = 24.4p = 0.004 | |

A
_{4} | Linear regression | Male mice, CPP score vs VTA pDA α6* nAChR upregulation | R^{2} = 0.74F_{(1,5)} = 14.3p = 0.013 | |

B
_{1} | Linear regression | Female mice, CPP score vs VTA pDA α4α6* nAChR upregulation | R^{2} = 0.82F_{(1,6)} = 28.2p = 0.002 | |

B
_{2} | Linear regression | Female mice, CPP score vs VTA pDA α4* nAChR upregulation | R^{2} = 0.81F_{(1,6)} = 25.6p = 0.002 | |

B
_{3} | Linear regression | Female mice, CPP score vs VTA pDA α6* nAChR upregulation | R^{2} = 0.07F_{(1,6)} = 0.47p = 0.52 | |

C
_{2} | Linear regression | CPP score vs VTA pDA α4α6* nAChR upregulation | R^{2} = 0.08F_{(1,3)} = 0.24p = 0.65 | |

C
_{3} | Linear regression | CPP score vs VTA pDA α4* nAChR upregulation | R^{2} = 0.08F_{(1,3)} = 0.26p = 0.64 | |

C
_{4} | Linear regression | CPP score vs VTA pDA α6* nAChR upregulation | R^{2} < 0.01F_{(1,3)} = 0.01p = 0.94 | |

4 |
A
_{2} | Two-way ANOVA interaction | α4* nAChR upregulation | F_{(1,27)} = 0.99p = 0.33 |

Two-way ANOVA sex factor | α4* nAChR upregulation | F_{(1,27)} = 2.86p = 0.10 | ||

Two-way ANOVA drug factor | α4* nAChR upregulation | F_{(1,27)} = 17.1p = 0.0003 | ||

Two-way ANOVA means comparison | Male mice (α4* RID, saline vs nicotine) | p = 0.0012 | ||

Two-way ANOVA means comparison | Female mice (α4* RID, saline vs nicotine) | p = 0.05 | ||

A
_{3} | Linear regression | Male CPP score vs SNr GABA α4* nAChR upregulation | R^{2} = 0.55F_{(1,6)} = 7.39p = 0.04 | |

A
_{4} | Linear regression | Female CPP score vs SNr GABA α4* nAChR upregulation | R^{2} = 0.56F_{(1,6)} = 6.45p = 0.05 | |

B
_{2} | Two-way ANOVA interaction | α4* nAChR upregulation | F_{(1,24)} = 0.43p = 0.52 | |

Two-way ANOVA sex factor | α4* nAChR upregulation | F_{(1,24)} = 0.38p = 0.55 | ||

Two-way ANOVA drug factor | α4* nAChR upregulation | F_{(1,24)} = 14.0p = 0.001 | ||

Two-way ANOVA means comparison | Male mice (α4* RID, saline vs nicotine) | p = 0.04 | ||

Two-way ANOVA means comparison | Female mice (α4* RID, saline vs nicotine) | p = 0.013 | ||

B
_{3} | Linear regression | Male CPP score vs SNr GABA α4* nAChR upregulation | R^{2} < 0.001F_{(1,6)} < 0.001p = 0.99 | |

B
_{4} | Linear regression | Female CPP score vs SNr GABA α4* nAChR upregulation | R^{2} = 0.075F_{(1,6)} = 0.40p = 0.55 | |

D
_{1} | Unpaired t test | Dentate Gyrus α4* nAChR upregulation, saline vs nicotine | p = 0.047 | |

D
_{2} | Linear regression | CPP score vs dentate gyrus α4* nAChR upregulation | R^{2} = 0.02F_{(1,4)} = 0.09p = 0.78 | |

5 |
C
| Unpaired t test | CPP score, saline vs nicotine | p = 0.026 |

E
| Unpaired t test | VTA pDA neuron firing frequency, saline vs nicotine | p = 0.0094 | |

F
| Linear regression | CPP Score vs VTA pDA neuron firing frequency | R^{2} = 0.65F_{(1,16)} = 11.2p = 0.016 | |

6 |
D
| Unpaired t test | VTA pGABA neuron firing frequency, saline vs nicotine | p = 0.0056 |

E
| Linear regression | CPP Score vs VTA pGABA neuron firing frequency | R^{2} = 0.77F_{(1,16)} = 20.3p = 0.004 |